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101.
Afaf Eladl Yudai Yamaoki Shoko Hoshina Haruka Horinouchi Keiko Kondo Shou Waga Takashi Nagata Masato Katahira 《International journal of molecular sciences》2021,22(7)
Origin recognition complex (ORC) binds to replication origins in eukaryotic DNAs and plays an important role in replication. Although yeast ORC is known to sequence-specifically bind to a replication origin, how human ORC recognizes a replication origin remains unknown. Previous genome-wide studies revealed that guanine (G)-rich sequences, potentially forming G-quadruplex (G4) structures, are present in most replication origins in human cells. We previously suggested that the region comprising residues 413–511 of human ORC subunit 1, hORC1413–511, binds preferentially to G-rich DNAs, which form a G4 structure in the absence of hORC1413–511. Here, we investigated the interaction of hORC1413-511 with various G-rich DNAs derived from human c-myc promoter and telomere regions. Fluorescence anisotropy revealed that hORC1413–511 binds preferentially to DNAs that have G4 structures over ones having double-stranded structures. Importantly, circular dichroism (CD) and nuclear magnetic resonance (NMR) showed that those G-rich DNAs retain the G4 structures even after binding with hORC1413–511. NMR chemical shift perturbation analyses revealed that the external G-tetrad planes of the G4 structures are the primary binding sites for hORC1413–511. The present study suggests that human ORC1 may recognize replication origins through the G4 structure. 相似文献
102.
Keiichirou Kusakari Masahiko Sakai 《Applicable Algebra in Engineering, Communication and Computing》2007,18(5):407-431
We enhance the dependency pair method in order to prove termination using recursive structure analysis in simply-typed term
rewriting systems, which is one of the computational models of functional programs. The primary advantage of our method is
that one can exclude higher-order variables which are difficult to analyze theoretically, from recursive structure analysis.
The key idea of our method is to analyze recursive structure from the viewpoint of strong computability. This property was
introduced for proving termination in typed λ-calculus, and is a stronger condition than the property of termination. The
difficulty in incorporating this concept into recursive structure analysis is that because it is defined inductively over
type structure, it is not closed under the subterm relation. This breaks the correspondence between strong computability and
recursive structure. In order to guarantee the correspondence, we propose plain function-passing as a restriction, which is
satisfied by many non-artificial functional programs. 相似文献
103.
A commercial multistage batch system electrodialysis plant for desalination of brackish groundwater was installed at Hatsushima, Atami City in April 1973. The plant has three stages each of which is operated at a constant voltage. Each stage has a dialyzer which is provided with 150 pairs of ion exchange membranes having an effective area of 1 m2 each. This plant is used for drinking water supply and has a capacity of 200 m3/day when the raw water has a saline concentration of 6,000 ppm TDS. The operation was very stable and easy, and it was possible to produce water of constant quality at all times regardless of fluctuations in the raw water concentration. The dialyzer was chemically cleaned for removal of deposits on membranes thereby reducing the maintenance cost by 62% without adversely influencing ion exchange membranes. 相似文献
104.
Shintaro Morisada Ken-ichiro Namazuda Haruka Kanda Yoshitsugu Hirokawa Yshio Nakano 《Advanced Powder Technology》2010,21(1):28-33
The adsorption and desorption behaviors of bovine serum albumin (BSA) in water for temperature-responsive polymer gel particles have been investigated by the temperature-swing operation between 298 and 313 K, where the cationic N-isopropylacrylamide (NIPA) gels copolymerized with vinylbenzyl trimethylammonium chloride (VBTA) or 2-(dimethylamino)ethyl methacrylate (DMAEMA) were used. The NIPA-VBTA and the NIPA-DMAEMA copolymer gels adsorbed BSA while the NIPA homopolymer gel hardly adsorbed BSA, indicating that the copolymer gels adsorb BSA through the electrostatic attraction between the positively charged groups in the gels and the negatively charged BSA. The adsorption amounts for the NIPA-DMAEMA gels were smaller than those for the NIPA-VBTA gels. This may be because almost every VBTA group, which is a quaternary ammonium salt, can be positively charged in water, while only some of the tertiary amine DMAEMA groups are protonated in water. Moreover, it was found that both the copolymer gels with a large mesh size of the polymer network repeatedly adsorbed BSA at 298 K and desorbed some of pre-adsorbed BSA at 313 K by the temperature-swing operation. This BSA desorption may result from the decrease of the number of the positively charged groups accessible to BSA due to the shrinking of the constituent polymer chains. 相似文献
105.
Haruka Dan Kazutake Okuhara Kaoru Kohyama 《Journal of the science of food and agriculture》2005,85(5):785-790
This study sought to statistically analyze the difference of planar stress distribution in a cross section of cucumber cultivars. The method dealt with statistically comparable data, incorporating both the stress magnitude and their positional information, and was developed to reduce complexity without losing anatomical information. Utilizing the trigonal symmetry of cross‐sectioned cucumbers, a reference line bisecting the cross section was introduced on the rupture frame for referencing the relative locations of stress‐sensing points to the underlying anatomical tissue distribution. The stress–location curves on the reference line statistically differed among the cultivars, especially at the center of the cross section. The corresponding tissue for the center of the curve was the seed cavity, so the cultivar differences arise from mainly the mechanical differences in this tissue. The stress–strain analysis of definite tissues revealed flesh stiffness to exceed the seed cavity stiffness, but both differed from the average stiffness of the whole cross section in all cultivars. Copyright © 2004 Society of Chemical Industry 相似文献
106.
Haruka Dan Kazutake Okuhara Kaoru Kohyama 《Journal of the science of food and agriculture》2004,84(10):1091-1096
Planar stress distribution of cucumber fruits was visualized for four cultivars. The planer stress distribution of a cross‐sectional sample was measured using a multiple‐point sheet sensor during uniaxial compression by an Instron universal testing machine. The concentric distribution pattern of stress gradients was the common characteristic for all cultivars. The correspondence between the tissue and its mechanical properties was estimated by comparing the stress distribution and a sample photograph. The stress property differed among different tissues within the cross‐section. The center of the cross‐sectional slice, where the seed cavity occupies formed the lower stress region, the surrounded area, where the flesh locates showed the middle‐range stress, and the exterior skin exhibited the highest stress. The wider distribution of the lower stress was statistically significant for two cultivars. This visualization method provided qualitative information about the geometrical and mechanical distribution of the cucumber and allowed the breed specificity of the cucumber cultivar to be characterized. Copyright © 2004 Society of Chemical Industry 相似文献
107.
Haruka Dan Kazutake Okuhara Kaoru Kohyama 《Journal of the science of food and agriculture》2006,86(1):26-34
The fracture process of six cucumber cultivars was examined to explore the mechanical properties that determine cultivar‐specific crispy and crunchy textures. The postfailure portion of the load–strain curves was targeted in cucumber compression tests. The series of fractures after the initial fracture were examined at the tissue level by expressing the two‐dimensional stress intensity as a map. The stress map for the difference between the fracture point and 0.01 s after the fracture point indicated that the stress distribution changed in a broad area for two cultivars (D and F), while it changed locally and in a small area for two other cultivars (A and E). Several load drops were observed on the load–strain curve for the cucumbers. The peak number between the fracture and the local minimal points was quite small for cultivars D and F, whereas it was substantial for cultivars A and E. A global fracture event occurred over the entire cross‐sectional area at the initial fracture for cultivars D and F. The initial fracture occurred locally for cultivars A and E, and there were repeated sequential small local fractures as sample compression progressed. Two other cultivars (B and C), typical of those normally eaten raw, showed intermediate values for most parameters. This measurement provided the first demonstration of structural fracture during a series of load drops. How these postfailure properties will be associated with the texture characteristics of cucumber cultivars is discussed. Copyright © 2005 Society of Chemical Industry 相似文献
108.
Accurate predictions of the mechanical response of root-soil systems are required for assessing and reducing the risk of landslides, surface erosions, and lodging. The present paper proposes the use of the node-to-segment (NTS) approach with the finite element method for predicting contact phenomena between roots and soils, such as collision, sliding, and separation. To obtain reliable predictions for the deformation of such geometrically complex problems, a stabilizing algorithm within the NTS approach is proposed and implemented here. The proposed algorithm prevents the well-known non-uniqueness problem of the pairing algorithm in the NTS approach, which has been an obstacle to applying the approach to root-soil systems. The current method is employed for two numerical examples. The first is an example of validation, in which pullout experiments are re-analyzed to examine the applicability of the method to a geometrically simple root-soil contact problem. It is shown that the current method provides a reasonable prediction of the pullout response, and that both the friction and the cohesion can also be accurately estimated with it. The second is an example of a realistic problem, in which a 2-D lodging experiment, analogous to pile-loading problems, is conducted and simulated to demonstrate the accuracy and applicability of the NTS approach in plant-scale problems with complex root geometries. The relationship between the displacement and the reaction force of the simulation is consistent with that of the experiment, and it enables the visualization of the stress contour and deformation of the rhizosphere. 相似文献
109.
Haruka Nishibata;Takuto Miyatake;Yamato Matsuda;Md. Azhar Uddin;Yoshiei Kato;Akifumi Harada; 《国际钢铁研究》2024,95(1):2300331
To discuss nonmetallic inclusion behaviors in a secondary refining process, cold model experiments on agglomeration, breakup, and transfer of fine particles are performed varying the operating factors such as an energy input rate, wettability, agitation practice, and the number of liquid phases. When the other operating conditions are the same, the agglomeration rate of the 1-particle (isolated particle) with a hydrophobic property is larger than that with a hydrophilic one, and the transfer rate of the 2-particle (cluster of 2 isolated particles) has the same tendency. The number of the 1-particle with the hydrophobicity is estimated to increase at the transfer site of the particles from an open eye to a heavy liquid due to a bubble breakup, whereas the 1-particle with the hydrophilicity shows an opposite trend because of less adhesion on the bubbles. The 1-particle in the dual phase transfers from the open-eye spot to a light liquid, although the value is not so much as that of the decrease in the agglomeration. A transfer condition of the inclusion composed of Al2O3 or CaO⋅Al2O3 is calculated when a particle contacts with a solid/liquid interface, and the CaO⋅Al2O3 inclusion is estimated to be engulfed above 3.34 μm in diameter. 相似文献
110.
Hiroaki Tsurumaki Chihiro Mogi Haruka Aoki-Saito Masayuki Tobo Yosuke Kamide Masakiyo Yatomi Koichi Sato Kunio Dobashi Tamotsu Ishizuka Takeshi Hisada Masanobu Yamada Fumikazu Okajima 《International journal of molecular sciences》2015,16(12):28931-28942
Acute lung injury is characterized by the infiltration of neutrophils into lungs and the subsequent impairment of lung function. Here we explored the role of TDAG8 in lung injury induced by lipopolysaccharide (LPS) administrated intratracheally. In this model, cytokines and chemokines released from resident macrophages are shown to cause neutrophilic inflammation in the lungs. We found that LPS treatment increased TDAG8 expression in the lungs and confirmed its expression in resident macrophages in bronchoalveolar lavage (BAL) fluids. LPS administration remarkably increased neutrophil accumulation without appreciable change in the resident macrophages, which was associated with increased penetration of blood proteins into BAL fluids, interstitial accumulation of inflammatory cells, and damage of the alveolar architecture. The LPS-induced neutrophil accumulation and the associated lung damage were enhanced in TDAG8-deficient mice as compared with those in wild-type mice. LPS also increased several mRNA and protein expressions of inflammatory cytokines and chemokines in the lungs or BAL fluids. Among these inflammatory mediators, mRNA and protein expression of KC (also known as CXCL1), a chemokine of neutrophils, were significantly enhanced by TDAG8 deficiency. We conclude that TDAG8 is a negative regulator for lung neutrophilic inflammation and injury, in part, through the inhibition of chemokine production. 相似文献