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41.
The photoionization cross-sections of various deep impurities of interest in solar-grade silicon for photovoltaic cells, and
the corresponding energy levels, have been determined by steady state photo-induced currents in pn junctions or Schottky barrier
junctions irradiated simultaneously with two wavelengths of light. Light of about half the band-gap energy controls the occupancy
of the deep impurity level and the spectral dependence of the photocurrent on a higher photon energy light source then provides,
via the Lucovsky model, the photo-cross-section and the impurity energy level. The results obtained for Au and Pt in Si are
in agreement with those of Braun and Grimmeiss and the energy levels for Fe, Ti, and Ag obtained optically are in agreement
with those obtained by other methods. 相似文献
42.
Higashi T Nagamori E Sone T Matsunaga S Fukui K 《Journal of Bioscience and Bioengineering》2004,97(3):191-195
The direct transfer of genetic materials into mammalian cells is an indispensable technique. We have developed calcium alginate (CA) microbeads which can deliver plasmid DNAs and yeast artificial chromosomes into plant and yeast cells. In this paper, we demonstrate the effective transfection of mammalian cells by CA microbeads immobilizing plasmid DNAs. The transfection was performed using the pEGFP-C1 plasmid containing the cytomegalovirus (CMV) promoter and enhanced green fluorescent protein (EGFP) gene. The transient expression of EGFP was observed 24 h after transfection. The expression efficiency was maximum when the concentration of sodium alginate was 1% and the amount of plasmid DNA was increased to 100 microg. The expression efficiency of our method using CA microbeads is 2-10 times higher than that of the polyethylene glycol (PEG) method. Our results suggest that the CA microbead mediated transfection of mammalian cells effectively delivers genetic materials into mammalian suspension cells. 相似文献
43.
Matsunaga S Ohshio K Harada E Fujiwara S Uchiyama S Fukui K 《Journal of Bioscience and Bioengineering》2004,98(5):384-386
We applied fluorescent microscopy to monitor the damage of DNA upon exposure to gamma radiation. Our developed dosimetry demonstrated that the number of breaks in DNA is proportional to the dose of the irradiation but is not dependent on dose rate of the irradiation and the GC content of DNA. 相似文献
44.
Shortencarier MJ Dayton PA Bloch SH Schumann PA Matsunaga TO Ferrara KW 《IEEE transactions on ultrasonics, ferroelectrics, and frequency control》2004,51(7):822-831
We have developed a method using ultrasound and acoustically active lipospheres (AALs) that might be used to deliver bioactive substances to the vascular endothelium. The AALs consist of a small gas bubble surrounded by a thick oil shell and enclosed by an outermost lipid layer. The AALs are similar to ultrasound contrast agents: they can be nondestructively deflected using ultrasound radiation force, and fragmented with high-intensity ultrasound pulses. The lipid-oil complex might be used to carry bioactive substances at high concentrations. An optimized sequence of ultrasound pulses can deflect the AALs toward a vessel wall then disrupt them, painting their contents across the vascular endothelium. This paper presents results from a series of in vitro and ex vivo experiments demonstrating localization of a fluorescent model drug. In experiments using a human melanoma cell (A2085) monolayer, a specific radiation force-fragmentation ultrasound pulse sequence increased cell fluorescence more than 10-fold over no ultrasound or fragmentation pulses alone, and by 50% over radiation force pulses alone. We observe that dye transfer is limited to cells that are in the region of ultrasonic focus, indicating that the application of radiation force pulses to bring the delivery vehicle into proximity with the cell is required for successful adhesion of the vehicle fragments to the cell membrane. We also demonstrate dye transfer from flowing AALs, both in a mimetic vessel and in excised rat cecum. We believe that this method could be successfully used for drug delivery in vivo. 相似文献
45.
Mechanical and thermophysical characteristics of Si-Ti-C-O fibre-bonded ceramic produced by hot-pressing the laminated material of oxidized satin-woven Si-Ti-C-O fibre have been investigated at room and cryogenic temperatures. The fibre element (diameter: 8 m, fibre volume fraction: 85 ± 1%) constructing the Si-Ti-C-O fibre-bonded ceramic showed a close-packed structure of the oxidized Si-Ti-C-O fibre mainly composed of fine SiC crystals, amorphous SiO2-based phase and turbostratic carbon. The Si-Ti-C-O fibre-bonded ceramic with lightweight (density: 2.45 × 103kg/m3) and low porosity (<1 vol%) showed a markedly higher fracture energy (notched, cross-plied specimen: approximately 10kJ/m2) and lower thermal conductivity (1/10 the value of stainless steel). The reason why the fibre- bonded ceramic showed such a low thermal conductivity in spite of very high thermal conductivity of a pure SiC and carbon could be attributed to the complicated microstructure of Si-Ti-C-O fibre-bonded ceramics. 相似文献
46.
A recirculating system of aqueous trichloroethylene (TCE) solutions through the packed bed reactor with TiO2 pellets has been developed in order to mineralize TCE without difficulties for filtration and recovery of catalyst. The TiO2 pellets prepared by sol gel method have photocatalytic activity similar to commercially available PC-101 and PC-102 in the powder form and to ST-B11 pellets. In batch experiments with TiO2 powders, Degussa P-25 is the most active photocatalyst, which indicates that specific surface area is not an important factor controlling the photocatalytic activity in aqueous solutions. The degradation rates of TCE in the recirculating system with TiO2 pellets decreased in the presence of H2O2, while were remarkably accelerated by adding S2O8(2-). The presence of S2O8(2-) ions more than 0.01 mol dm(-3) completely suppressed hole-electron recombination and mineralized 50 ppm TCE with the 2 h irradiation. In a reactor without TiO2 photocatalysts, TCE was photodegraded by SO4- radicals which produced by photodissociation of S2O8(2-). The degradation rates increased with increase of the initial S2O8(2-) concentration. However, TCE was not mineralized but converted to intermediates which were slowly degraded to Cl- by continuing the irradiation. 相似文献
47.
Guoqing Guan Katsuki Kusakabe Haruka Ozono Masatsugu Taneda Masato Uehara Hideaki Maeda 《Journal of Materials Science》2007,42(24):10196-10202
Three-dimensionally (3D) well-ordered carbon microparticle assemblies with different particle morphologies were fabricated
by infiltration of phenolic resin solution into SiO2 inverse opal structures and subsequent carbonization. The effect of the phenolic resin solution concentration and the carbonization
temperature on the morphology of the fabricated carbon microparticles was investigated. At a carbonization temperature of
1000 °C, carbon microparticles with interlocked bridges were obtained when the concentration of phenolic resin solution is
40 wt% and hollow carbon microparticles with opened window channels were obtained at a concentration of 30–35 wt%. When the
carbonization temperature was decreased to 500 °C, carbon microparticles with interlocked bridges also were observed, even
when the phenolic resin concentration was 30 wt%. The structures and properties of the carbon microparticles and their assemblies
were characterized using SEM, XRD, and N2 adsorption. 相似文献
48.
A.J. McEvily S. Ishihara M. Endo H. Sakai H. Matsunaga 《International Journal of Fatigue》2007,29(12):2237-2245
Recent data on short fatigue crack growth in two cast and hot isostatically pressed (hipped) aluminum alloys obtained by Shyam, Allison and Jones have been analyzed in terms of a previously proposed one-parameter short crack model which includes consideration of elastic–plastic effects, the Kitagawa effect and the development of crack closure in the wake of a newly formed crack. The material constants obtained in a prior investigation of short crack growth behavior in a cast aluminum alloy tested under fully reversed loading were used as a basis for the present analysis. The predicted rates of fatigue crack propagation are in accord with the experimental results. In the discussion, aspects of the two-parameter approach presented by Shyam et al. are compared with those of the one-parameter method of analysis used herein. 相似文献
49.
An N*N self-routing planar network suitable for fabricating optical space switches on lithium niobate substrate is presented. The network has O(N/sup 2/) cross points, good modularity and expandability. It also has no waveguide crossovers and no differential attenuation between any of the input and output pairs. A self-routing control can be applied to this network, which is indispensable for ATM switching systems.<> 相似文献
50.
Katsuyuki Matsunaga Fumiyasu Oba Isao Tanaka Hirohiko Adachi 《Journal of Electroceramics》1999,4(1):69-80
Discrete variational (DV) Xα cluster method has been employed in calculating electronic structures of ZnO. Electronic structures of the bulk and the non-polar surface model clusters are calculated with inclusion of electrostatic potentials in the bulk and near the surface, and the electronic origins of experimental spectra and chemical bonds at the surface are examined in detail. The valence band structure constructed by Zn-3d and O-2p bands is much influenced by electrostatic potentials in ZnO. It is found that the reduction of an electrostatic potential near the surface gives rise to the difference of the valence band structures between in the bulk and at the surface. The calculated density of states at the non-polar surface of ZnO, where the Zn-3d and O-2p bands are more widely separated than in the bulk, is in good agreement with the experimental UPS. In addition, a Zn-O bond at the surface is found to show stronger covalency than that in the bulk, as a result of the change of the valence band structure due to the effect of the electrostatic potential. 相似文献