A novel immunostimulating aspect of Lactobacillus gasseri: induction of "Gasserokine" as chemoattractants for macrophages

The chemotactic activity of the culture supernatants from 14 strains of Lactobacillus acidophilus and L. gasseri was examined for murine macrophages. Significant macrophage chemotactic activity was observed in three strains of L. acidophilus and all strains of L. gasseri. The highest activity was observed in the supernatant (1131-sup) from 24-h cultures of L. gasseri JCM1131T. The chemotactic factor from 1131-sup, designated as "Gasserokine", was purified by the C18 reverse phase and ion-exchange chromatography. The purity of Gasserokine was checked by HPLC with the reverse-phase mode. The chemotactic activity of Gasserokine was also observed for human monocytes. The macrophage chemotaxis induced by L. gasseri JCM1131T culture supernatants was discovered to be a new biological function exerted by probiotic lactic acid bacteria. Therefore, the activity is expected to be used for one of the functional parameters in the immunomodulating properties of probiotic lactic acid bacteria.     

A frame-based knowledge software tool for developing interactive robots

A knowledge-based software tool for developing interactive robot applications, called SPAK, has been developed. The “world” of interest is represented in a SPAK knowledge base by using a frame knowledge technique. This technique is chosen because it can represent the world meaningfully and naturally. Relationships among frames, which represent things in the world, and actions to be taken when certain things occur can be specified. In action, SPAK perceives changes in the environment, updates the knowledge base if needed, and generates output actions according to the knowledge contents. To support robotic applications, extensions to the conventional frame model are proposed. Various robotic applications can run cooperatively on top of SPAK. Each can easily make use of the knowledge available, and share its knowledge with others. A SPAK knowledge editor allows simple and intuitive development and modification of robot applications. To demonstrate these benefits, a prototype system and a sample robot application are developed. A multiagent technique is employed to combine various robotic components, both hardware and software, together. A sample dialogue manager for managing interactions with humans runs as an application on SPAK.     

Preferred crystal orientation of sol-gel-derived Bi4-xLaxTi3O12 thin films on silicon substrates

Polycrystalline thin films of La-substituted bismuth titanate (BLT) were formed directly on p-type Si(100) substrates by using sol-gel and spin coat methods. The BLT film and interfacial layer between BLT and Si were quantitatively investigated by the X-ray reflectivity method. Also, crystal orientations of sub-100-nm-thick BLT thin films were confirmed by X-ray diffraction using a synchrotron radiation source. The preferred c-axis orientation normal to the surface depended on the crystallization temperature. The difference in the preferred c-axis orientations of the BLT films caused the difference in the hysteresis voltage width in the capacitance-voltage characteristics of Au/BLT/p-Si structures. Furthermore, the c-axis of the Bilayered structure was preferentially oriented and aligned in the in-plane direction.     

Isolation and characterization of long-chain-alkane degrading Bacillus thermoleovorans from deep subterranean petroleum reservoirs

Two extremely thermophilic alkane-degrading bacterial strains, B23 and H41, were respectively isolated from deep subterranean petroleum reservoirs in the Minami-aga (Niigata) and Yabase (Akita) oil fields. Both strains were able to grow at temperatures ranging from 50 to 80 degrees C, with optimal growth at 70 degrees C for B23 and 65 degrees C for H41. From 16S rRNA gene sequence analysis and physiological characterization, both strains were identified as Bacillus thermoleovorans (identities of 99.5% and 99.6% to strain DSM 5366, and 98.3% and 98.7% to the type strain LEH-1(TS), respectively). Strains B23 and H41 effectively (60%) degraded n-alkanes longer than C12 and C15, respectively, at 70 degrees C, while strain LEH-1(TS) degraded undecane (C11) most effectively. When B23 and H41 were cultivated in the presence of heptadecane, heptadecanoate and pentadecanoate were specifically accumulated in the cells. These results strongly suggest that the two strains degraded n-alkanes by a terminal oxidation pathway, followed by a beta-oxidation pathway.     

The Immune Response in Adipocytes and Their Susceptibility to Infection: A Possible Relationship with Infectobesity

The current obesity pandemic has been expanding in both developing and developed countries. This suggests that the factors contributing to this condition need to be reconsidered since some new factors are arising as etiological causes of this disease. Moreover, recent clinical and experimental findings have shown an association between the progress of obesity and some infections, and the functions of adipose tissues, which involve cell metabolism and adipokine release, among others. Furthermore, it has recently been reported that adipocytes could either be reservoirs for these pathogens or play an active role in this process. In addition, there is abundant evidence indicating that during obesity, the immune system is exacerbated, suggesting an increased susceptibility of the patient to the development of several forms of illness or death. Thus, there could be a relationship between infection as a trigger for an increase in adipose cells and the impact on the metabolism that contributes to the development of obesity. In this review, we describe the findings concerning the role of adipose tissue as a mediator in the immune response as well as the possible role of adipocytes as infection targets, with both roles constituting a possible cause of obesity.     

Total Syntheses of Useful Bioactive Compounds

The first total syntheses of a variety of useful bioactive compounds have been accomplished by practically useful strategies. In addition practically useful intermediates have been developed, analogues of natural products have been prepared, their structure‐activity relationships studied, and the large‐scale preparations of medically useful compounds established. The target molecules are antibiotics, antifungals, antitumor antibiotics, compounds related to β‐lactam antibiotics, enzyme inhibitors, central nervous system‐affecting products, non‐steroidal progesterone receptor ligands, mussel‐attachment inhibitors, and so on.     

Implementation and evaluation of a remote authentication system using touchless palmprint recognition

When a cellular phone is lost or stolen, it may be used improperly or the personal information may be stolen from it by a malicious user. Biometric authentication such as palmprint recognition is the strongest of the personal authentication technologies designed to prevent such misuse. In biometric authentication, when compared with a local authentication model, a remote authentication model has several advantages such as direct authentication and authentication levels. Ito et al. proposed several palmprint recognition schemes using correspondence matching based on the phase-only correlation. However, these schemes require a palmprint image to be captured with the hand touching the dedicated device, while palmprint images must be captured without such physical contact when using cellular phones. Thus, these schemes cannot be applied to cellular phones since there are large positioning gaps and large differences in brightness and distortion between the images. Furthermore, they have not been implemented in cellular phones and their performances have not been evaluated either. In this paper, we adopt a remote authentication model from the two types of biometric authentication incorporating the above advantages and propose a remote system between a cellular phone and an authentication server. We implement the proposed system using two different types of Android terminal as the terminal on the user side. We also show the validity of the proposed system by examining and confirming the accuracy and processing time. We furthermore discuss the problem of an impersonation attack on the proposed system and consider solutions to this problem from the viewpoints of security and usability. Then, we adopt a palmprint recognition scheme as a biometric authentication scheme and, in particular, use a palmprint recognition algorithm that incorporates Yörük et al.’s preprocessing technique to Ito et al.’s and Iitsuka et al.’s schemes.     

Identification of the genes encoding Mn2+-dependent RNase HII and Mg2+-dependent RNase HIII from Bacillus subtilis: classification of RNases H into three families

Database searches indicated that the genome of Bacillus subtilis contains three different genes encoding RNase H homologues. The ypdQ gene encodes an RNase HI homologue with 132 amino acid residues, whereas the rnh and ysgB genes encode RNase HII homologues with 255 and 313 amino acid residues, respectively. RNases HI and HII show no significant sequence similarity. These genes were individually expressed in Escherichia coli; the recombinant proteins were purified, and their enzymatic properties were compared with those of E. coli RNases HI and HII. We found that the ypdQ gene product showed no RNase H activity. The 2.2 kb pair genomic DNA containing this gene did not suppress the RNase H deficiency of an E. coli rnhA mutant, indicating that this gene product shows no RNase H activity in vivo as well. In contrast, the rnh (rnhB) gene product (RNase HII) showed a preference for Mn2+, as did E. coli RNase HII, whereas the ysgB (rnhC) gene product (RNase HIII) exhibited a Mg2+-dependent RNase H activity. Oligomeric substrates digested with these enzymes indicate similar recognition of these substrates by B. subtilis and E. coli RNases HII. Likewise, B. subtilis RNase HIII and E. coli RNase HI have generated similar products. These results suggest that B. subtilis RNases HII and HIII may be functionally similar to E. coli RNases HII and HI, respectively. We propose that Mn2+-dependent RNase HII is universally present in various organisms and Mg2+-dependent RNase HIII, which may have evolved from RNase HII, functions as a substitute for RNase HI.     

Efficient cleavage of RNA at high temperatures by a thermostable DNA-linked ribonuclease H

To construct a DNA-linked RNase H, which cleaves RNA site-specificallyat high temperatures, the 15-mer DNA, which is complementaryto the polypurine-tract sequence of human immunodeficiency virus-1RNA (PPT-RNA), was cross-linked to the unique thiol group ofCys135 in the Thermus thermophilus RNase HI variant. The resultantDNA-linked enzyme (d15-C135/TRNH), as well as the d15-C135/ERNH,in which the RNase H portion of the d15-C135/TRNH is replacedby the Escherichia coli RNase HI variant, cleaved the 15-merPPT-RNA site-specifically. The mixture of the unmodified enzymeand the unlinked 15-mer DNA also cleaved the PPT-RNA but ina less strict manner. In addition, this mixture cleaved thePPT-RNA much less effectively than the DNA-linked enzyme. Theseresults indicate that the cross-linking limits but acceleratesthe interaction between the enzyme and the DNA/RNA substrate.The d15-C135/TRNH cleaved the PPT-RNA more effectively thanthe d15-C135/ERNH at temperatures higher than 50°C. Thed15-C135/TRNH showed the highest activity at 65°C, at whichthe d15-C135/ERNH showed little activity. Such a thermostableDNA-linked RNase H may be useful to cleave RNA molecules withhighly ordered structures in a sequence-specific manner.     

Earthquake-related Natech risk assessment using a Bayesian belief network model

Natural hazard triggering technological disasters (Natech) events pose risks to industrial facilities and process plants. As these plants handle hazardous materials, they can endanger nearby residential areas and have financial consequences. Thus, proper Natech risk assessment is required for effective prevention, mitigation and emergency response planning at industrial plants and nearby residential areas. The parameters used to quantify Natech risk assessment are subject to uncertainties and their interactions are non-linear. In this study, a Bayesian belief network-based Natech risk assessment model is developed to assess the earthquake-related Natech risk considering different levels of uncertainties. The cause and effect relationships between different parameters are constructed based on published body of knowledge and expert knowledge. The capabilities of the proposed model are demonstrated for the earthquake-related Natech risk assessment of Kobe City, Higashinada Ward, Japan because of the Great Hanshin earthquake in 1995. The proposed model is also capable of performing both predictive analysis and diagnostic analysis.