Kinetic masks: a new approach and device for dispersing biologically relevant fluids

The controlled dispersion of fluids, particularly biologically relevant solutions in micro-volumes, is of high practical interest in biotechnology and medicine. Pharmaceutical test assays, for example, need a method for the fast and defined deposition of fluid samples. Most current micro-dispensing methods, i.e. contact-based pin printing, have problems such as time delays, limited dosing velocity, minimum volume or high interference that limit biological applications. Spraying techniques suffer from a lack of reproducibility; a defined deposition of samples on targets is not possible. Here, we introduce a new method for the parallel and spatially defined dispersion of many micro-volumes that overcomes disadvantages of common micro-dispensers. The overall approach is that a fluid drop, produced by a droplet generator, falls on a free trajectory with a defined kinetic energy, and is split by a masking unit placed perpendicular to the flight direction into at least two smaller droplets (Zimmermann et al. in Method and device for dosing fluid media, WO/2002/102515, Germany, 2002). On the target, the resulting droplets form reproducible patterns, which are enlarged and scalable images of the grid pattern. Possible applications for this method are non-contact cell patterning, cell encapsulation, cryopreservation and fast mixing processes in micro-volumes. Here, we use this method for the direct and defined parallel positioning of cell suspensions on specific substrates, which can be useful for test assays, tissue engineering and cryopreservation.     

Agent-based negotiation and decision making for dynamic supply chain formation

Modern businesses are facing the challenge of effectively coordinating their supply chains from upstream to downstream services. It is a complex problem to search, schedule, and coordinate a set of services from a large number of service resources under various constraints and uncertainties. Existing approaches to this problem have relied on complete information regarding service requirements and resources, without adequately addressing the dynamics and uncertainties of the environments. The real-world situations are complicated as a result of ambiguity in the requirements of the services, the uncertainty of solutions from service providers, and the interdependencies among the services to be composed. This paper investigates the complexity of supply chain formation and proposes an agent-mediated coordination approach. Each agent works as a broker for each service type, dedicated to selecting solutions for each service as well as interacting with other agents in refining the decision making to achieve compatibility among the solutions. The coordination among agents concerns decision making at strategic, tactical, and operational level. At the strategic level, agents communicate and negotiate for supply chain formation; at the tactical level, argumentation is used by agents to communicate and understand the preferences and constraints of each other; at the operational level, different strategies are used for selecting the preferences. Based on this approach, a prototype has been implemented with simulated experiments highlighting the effectiveness of the approach.     

It Consumerization: Byod-Program Acceptance and its Impact on Employer Attractiveness

Many firms are considering ‘bring-your-own-device’ (BYOD) programs, under which their employees are allowed to bring their own devices to work and use them for both private and business purposes. This study examines what factors determine an employee's intention to participate in a corporate BYOD program and how such programs affect employer attractiveness. We approach our study of acceptance of corporate BYOD programs from the perspective of technology acceptance research. For this purpose, we propose a modified and extended UTAUT model. The model was tested by surveying students in their final term (n = 444). We show that performance expectancies have the strongest positive effect on intention, while perceived threats negatively impact intention. Finally, behavioural intention was positively associated with employer attractiveness, which leads to clear indications for companies considering establishing corporate BYOD programs. BYOD seems to play an increasingly important role in attracting and retaining future talent.     

Adsorption of polymers at nanowires

Low-energy structures of a hybrid system consisting of a polymer and an attractive nanowire substrate as well as the thermodynamics of the adsorption transition are studied by means of Monte Carlo computer simulations. Depending on structural and energetic properties of the substrate, we find different adsorbed polymer conformations, amongst which are spherical droplets attached to the wire and monolayer tubes surrounding it. We identify adsorption temperatures and the type of the transition between adsorbed and desorbed structures depending on the substrate attraction strength.     

TimeLapseAnalyzer: multi-target analysis for live-cell imaging and time-lapse microscopy

The direct observation of cells over time using time-lapse microscopy can provide deep insights into many important biological processes. Reliable analyses of motility, proliferation, invasive potential or mortality of cells are essential to many studies involving live cell imaging and can aid in biomarker discovery and diagnostic decisions. Given the vast amount of image- and time-series data produced by modern microscopes, automated analysis is a key feature to capitalize the potential of time-lapse imaging devices. To provide fast and reproducible analyses of multiple aspects of cell behaviour, we developed TimeLapseAnalyzer. Apart from general purpose image enhancements and segmentation procedures, this extensible, self-contained, modular cross-platform package provides dedicated modalities for fast and reliable analysis of multi-target cell tracking, scratch wound healing analysis, cell counting and tube formation analysis in high throughput screening of live-cell experiments. TimeLapseAnalyzer is freely available (MATLAB, Open Source) at http://www.informatik.uni-ulm.de/ni/mitarbeiter/HKestler/tla.     

DelosDLMS

DelosDLMS is a novel digital library management system (DLMS) that has been developed as an integration effort within the DELOS Network of Excellence, a European Commission initiative funded under its fifth and sixth framework programs. In this paper, we describe DelosDLMS that takes into account the recommendations of several activities that were initiated by DELOS including the DELOS vision for digital libraries (DLs). A key aspect of DelosDLMS is its novel generic infrastructure that allows the generation of digital library systems out of a set of basic system services and DL services in a modular and extensible way. DL services like feature extraction, visualization, intelligent browsing, media-type-specific indexing, support for multilinguality, relevance feedback and many others can easily be incorporated or replaced. A further key aspect of DelosDLMS is its robustness against failures and its scalability for large collections and many parallel user requests. We discuss the current status of an effort to build DelosDLMS, a Digital Library Management System that integrates in various ways several components developed by DELOS members and showcases a great variety of functionality that is outlined as part of the DELOS vision.     

Will virtual education initiatives succeed?

The use of information technology to enhance teaching and learning processes has been practiced for a number of years now. However, the rapid growth in the use of the Internet has led to a new dimension in interactive and collaborative learning anytime and anyplace dynamically. With the explosion of “virtual education initiatives”, the question of the feasibility and success criteria for such projects quickly arises. To address the question in an organized way, we propose a project assessment based on critical success factors. Hence, in this article, we draw on a widely recognized critical success factor framework. We (slightly) adjust the framework to fit the special characteristics of virtual education initiatives, and apply to one case study, namely the virtual education initiative at the Faculty of Business at the City University of Hong Kong. The results suggest that the past success of the case is due to the adherence to the large majority of critical success factors. However, it also outlines some areas of concern. The paper concludes with a discussion on the strength and limitations of virtual learning environment as well as future directions. This revised version was published online in July 2006 with corrections to the Cover Date.     

The electrical conductivity of graphite intercalated with superacid fluorides: experiments with antimony pentafluoride

The electrical conductivity in graphite measured normal to the crystallographic c-axis is observed to increase after intercalation with acid molecules which act as acceptors. This behaviour is regarded as the result of ionization of the acid molecule which, in turn, increases the positive current carriers in the host graphite. Since the carrier density depends on the degree of ionization of the acid, it follows that the stronger the acid the greater the increase in carrier concentration, and assuming no adverse mobility effects, the greater the electrical conductivity. The hydrogen fluoride-antimony pentafluoride system produces some of the strongest acid substances known. The experiments described here represent the initial examination of the electrical conductivity resulting from intercalation of this material into graphite. The experiments consisted of intercalating graphite powder with antimony pentafluoride in a copper tube and swaging the sheathed compound into wire. The measured conductivity of the graphite intercalation compound, when the copper conductivity is subtracted out and allowance is made for departure from ideal density, is about 1×10^{6 –1} cm^–1. This is approximately 40 times the conductivity of pristine graphite and more than one and a half times the conductivity of pure copper.     

Rheological characterization and thermal degradation of PTFE

PTFE (¯M _n=5×10⁶), when heated near the melting temperature (335 to 337 C) while in contact with carbon black, is characterized by an effective viscosity and a thermal stability which are orders of magnitude lower than those found in the absence of the contacting high surface area material. The penetration of the PTFE into the porous carbon black occurs by the spreading of a very thin polymer film followed by a thickening of this film with time at temperature until a limiting concentration is reached. The lower the average molecular weight of the PTFE, the more rapidly it penetrates into the porous material. Similar phenomena have been observed with high molecular weight PTFE heated near the melting temperature while contacting high surface area metal blacks or porous sintered metals.     

The Effectiveness of Early Head Start for 3-Year-Old Children and Their Parents: Lessons for Policy and Programs.

Early Head Start, a federal program begun in 1995 for low-income pregnant women and families with infants and toddlers, was evaluated through a randomized trial of 3,001 families in 17 programs. Interviews with primary caregivers, child assessments, and observations of parent-child interactions were completed when children were 3 years old. Caregivers were diverse in race-ethnicity, language, and other characteristics. Regression-adjusted impact analyses showed that 3-year-old program children performed better than did control children in cognitive and language development, displayed higher emotional engagement of the parent and sustained attention with play objects, and were lower in aggressive behavior. Compared with controls, Early Head Start parents were more emotionally supportive, provided more language and learning stimulation, read to their children more, and spanked less. The strongest and most numerous impacts were for programs that offered a mix of home-visiting and center-based services and that fully implemented the performance standards early. (PsycINFO Database Record (c) 2010 APA, all rights reserved)