Expanding the Toolbox of R-Selective Amine Transaminases by Identification and Characterization of New Members

Amine transaminases (ATAs) are used to synthesize enantiomerically pure amines, which are building blocks for pharmaceuticals and agrochemicals. R-selective ATAs belong to the fold type IV PLP-dependent enzymes, and different sequence-, structure- and substrate scope-based features have been identified in the past decade. However, our knowledge is still restricted due to the limited number of characterized (R)-ATAs, with additional bias towards fungal origin. We aimed to expand the toolbox of (R)-ATAs and contribute to the understanding of this enzyme subfamily. We identified and characterized four new (R)-ATAs. The ATA from Exophiala sideris contains a motif characteristic for d -ATAs, which was previously believed to be a disqualifying factor for (R)-ATA activity. The crystal structure of the ATA from Shinella is the first from a Gram-negative bacterium. The ATAs from Pseudonocardia acaciae and Tetrasphaera japonica are the first characterized (R)-ATAs with a shortened/missing N-terminal helix. The active-site charges vary significantly between the new and known ATAs, correlating with their diverging substrate scope.     

Catalytic performance of methacrylate‐based poly‐4‐(dialkylamino)‐pyridines: activity prediction via NMR shifts

The synthesis of the 4‐(dialkylamino)pyridine derivative 3‐(4‐(pyridin‐4‐yl)piperazin‐1‐yl)propyl methacrylate and its copolymerization with n‐butyl methacrylate are presented. The catalytic activity was evaluated in the acylation of tert‐butanol with acetic anhydride yielding tert‐butyl acetate. It is observed that the activity of polymer‐attached 4‐(dimethylamino)pyridine analogues correlates remarkably well with the chemical shift of the β‐pyridyl protons. Differences in catalytic efficiency result from distinct electronic densities of the pyridine ring, while embedding the catalytically active moiety into a polymeric structure has nearly no deleterious effect on the performance. © 2015 Society of Chemical Industry     

Hydroxyl‐functional polyurethanes and polyesters: synthesis,properties and potential biomedical application

This mini review gives an overview of the synthesis of polyurethanes and polyesters with pendant hydroxyl groups performed mainly in our group. Significant for the synthesis of most of these polymers with hydroxyl‐functional side groups is that no protection groups are needed. This is realised either by generating the OH group during polymer synthesis—demonstrated by the preparation of hydroxyl‐functional polyurethanes from dicarbonate monomers and diamines—or by using catalysts which discriminate between primary and secondary OH groups—demonstrated by the synthesis of hydroxyl‐functional polyesters based on malic acid using scandium triflate as catalyst. In addition, the potential of carbonate couplers for the synthesis of multifunctional polyurethane is presented. The application of such multifunctional polyurethanes as antimicrobial polymers and coatings is briefly discussed. Copyright © 2012 Society of Chemical Industry     

Methacrylated calix[4]arene phosphonic acids as functionalized crosslinker for radical polymerization

We describe the synthesis of 5,11,17,23‐tetra‐tert‐butyl‐25,27‐dioxypropylphosphonic acid‐26,28‐dimethacryloyloxy‐calix [4]arene and 5,11,17,23‐tetra‐tert‐butyl‐25‐oxypropylphosphonic acid‐27‐hydroxy‐26,28‐dimethacryloyloxy‐calix[4]arene starting from para‐tert‐butyl‐calix[4]arene. The complete reaction was proved by matrix‐assisted laser desorption ionization time of flight mass spectrometry and nuclear magnetic resonance spectroscopy. The influence of these compounds on the kinetics of the radical polymerization of methyl methacrylate was shown by dilatometry. Furthermore, the adhesive properties of dental adhesives containing these calix[4]arene derivatives were investigated. Copyright © 2012 Society of Chemical Industry     

Acronis: 10 Tipps für einen Disaster-Recovery-Plan

Datenschutz und Datensicherheit - DuD -     

Etablierung einer nationalen Rohstoffbuchhaltung und dafür notwendige Informationen und Informationsgüte

The influence of anthropogenic processes on global material flow systems increasingly dominates the influence of natural processes. In this context, the provision of detailed knowledge on material flow systems is relevant for decision makers in resource policy. This information can be provided in the form of one-year material balances or, if repeated over a period of consecutive years, in the form of national resource budgets. In national resource budgets, all relevant flows of a material through a national economy are balanced and displayed in neatly arranged diagrams. By updating these material balances over a series of years, the development of material stocks can be estimated. Upcoming challenges both regarding supply and disposal of materials can be identified. Comprehensive balances are useful for decision makers in resource policy and, moreover, as an information basis for future exploitation of anthropogenic resources. However, both availability of data and possibilities for assessing the reliability of data are very limited. In this work, a methodology for investigating and evaluating the information basis of national resource budgets is presented. The methodology includes procedures for systematic characterization of resource budget data and formal procedures for evaluating their reliability. From a scientific perspective, the methodology contributes to understanding material flow systems better. It also enables implementing procedures towards national resource budgets in an administrative context.     

Anteil erneuerbarer Energien und klimarelevante CO2-Emissionen aus der thermischen Verwertung von Abfällen in Österreich

Since combustible wastes usually consist of biogenic (e.g. paper, wood, food waste) and fossil organic matter (plastics), their thermal recovery results in climate neutral and climate relevant CO₂ emissions. Moreover, the fraction of biogenic materials in the waste feed is relevant for the amount of renewable energy produced. The latter has to be reported and might be subsidized according to national laws (e.g. based on European directive 2009/28/EG). The present study represents the first comprehensive evaluation of the share of biogenic and fossil materials in the waste feed of waste-to-energy (WTE) plants on a national basis. The Balance Method, which is patented on a European level by TU Wien, was applied to 10 out of 13 Austrian WTE plants (around 2.3 Mio tons of waste corresponding to around 88 % of the overall waste feed in Austrian WTE plants). The method is based on the mathematical reconciliation of the material properties (e.g. mean chemical composition of biogenic and fossil materials) and routinely recorded operating data of WTE plants (e.g. flue gas volume, CO₂ and O₂-content in the dry flue gas, steam production). The results demonstrate large variations for the share of energy from biogenic sources in the different WTE plants, ranging from 35.7 ± 2.4 % to 61.2 ± 2.7 % (based on annual averages). Additionally, for several WTE plants large temporal variations can be observed based on monthly mean values. Thus, a plant-specific and continuous evaluation of the waste composition in WTE plants (which the Balance Method allows to do at reasonable efforts) can be recommended for a reliable reporting of the renewable share of energy or fossil CO₂ emissions from waste incineration. The energy input which stems from fossil and biogenic sources can be estimated to 11,450 ± 120 TJ and 10,730 ± 110 TJ, respectively for the year 2014 (for the 10 WTE plants). In total 1060 ± 24 kt fossil CO₂ emissions from the thermal recovery of waste in Austria’s WTE plants in 2014 could be determined (estimation for all 13 WTE plants).     

Distribution and identification of culturable airborne microorganisms in a Swiss milk processing facility

Airborne communities (mainly bacteria) were sampled and characterized (concentration levels and diversity) at 1 outdoor and 6 indoor sites within a Swiss dairy production facility. Air samples were collected on 2 sampling dates in different seasons, one in February and one in July 2012 using impaction bioaerosol samplers. After cultivation, isolates were identified by mass spectrometry (matrix-assisted laser desorption/ionization-time-of-flight) and molecular (sequencing of 16S rRNA and rpoB genes) methods. In general, total airborne particle loads and total bacterial counts were higher in winter than in summer, but remained constant within each indoor sampling site at both sampling times (February and July). Bacterial numbers were generally very low (<100 cfu/m³ of air) during the different steps of milk powder production. Elevated bacterial concentrations (with mean values of 391 ± 142 and 179 ± 33 cfu/m³ of air during winter and summer sampling, respectively; n = 15) occurred mainly in the “logistics area,” where products in closed tins are packed in secondary packaging material and prepared for shipping. However, total bacterial counts at the outdoor site varied, with a 5- to 6-fold higher concentration observed in winter compared with summer. Twenty-five gram-positive and gram-negative genera were identified as part of the airborne microflora, with Bacillus and Staphylococcus being the most frequent genera identified. Overall, the culturable microflora community showed a composition typical and representative for the specific location. Bacterial counts were highly correlated with total airborne particles in the size range 1 to 5 µm, indicating that a simple surveillance system based upon counting of airborne particles could be implemented. The data generated in this study could be used to evaluate the effectiveness of the dairy plant’s sanitation program and to identify potential sources of airborne contamination, resulting in increased food safety.