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Interfacial hybridization kinetics of oligonucleotides immobilized onto fused silica surfaces 总被引:3,自引:0,他引:3
Jiang Zeng Amer Almadidy James Watterson Ulrich J. Krull 《Sensors and actuators. B, Chemical》2003,90(1-3):68-75
Fused silica optical fibers have been used in an intrinsic mode optical configuration as biosensors for fluorescence based detection of hybridization of nucleic acids. In this work, the kinetics of hybridization of single-stranded oligonucleotides that were covalently immobilized were studied. The probe DNA was dT20, and the target was Fluorescein-labeled non-complementary (dT20) or complementary (dA20) oligonucleotide. Chronofluorimetric monitoring of the adsorption and hybridization processes was used to investigate oligonucleotide films of different density, in different salt concentrations, at temperatures of 25 and 40 °C, with the concentration of the target DNA being 0.005–0.1 μM. Mathematical models based on first- and second-order Langmuir adsorption have been examined to describe both the adsorption and the hybridization processes. Experimental data were processed using the models, and the hybridization kinetics were calculated. Hybridization kinetics on these optical fiber DNA sensors was found to be up to three orders faster than results presented for a number of other experiments using different immobilization chemistries. 相似文献
64.
防火墙是提供网络安全性的重要手段之一,Linux提供了实现防火墙技术的工具包.分析了Linux系统中常用的包过滤防火墙技术--IPchains和IPtables.这两种防火墙技术作为两种基本的包过滤工具,实际上使用的是内核中的信息表,将来自非信任源的不必要的信息过滤出去.与IPchains相比,IPtables信息包过滤系统是最新的解决方案,也是第一个集成到Linux内核的解决方案.在许多方面,IPtables比IPchains灵活,它允许管理员控制更多的细节,例如记录日志等级. 相似文献
65.
就通信设计在通信工程建设中的重要地位进行了思考,分析了加入WTO以后我国通信设计行业面临的挑战与机遇,探讨了新形势下我国通信设计行业的应对措施及发展思路。 相似文献
66.
Longjie Zhou A. Zimmermann Yu-Ping Zeng F. Aldinger 《Journal of Materials Science》2004,39(8):2675-2681
The bipolar electric fatigue behaviour of antiferroelectric ceramics with the composition of Pb0.88Ba0.10La0.02(Zr0.55Sn0.35Ti0.10)O3 was investigated under various cycling fields. The material exhibits a degradation in the maximum field induced strain, a diffuse AFE-FE phase transition and an enhancement in the diffusion character of the FE-AFE phase transition due to electric cycling. Those variations increase with cycle number, indicating a logarithmic fatigue up to 108 cycles. There is no indication for the variations to be recovered, and the symmetry of the negative and positive parts of the strain hysteresis loops still remains. Higher cycling field results in a stronger deterioration of the maximum field induced strain and a larger extent of diffusion in AFE-FE and FE-AFE phase transitions. The normalized maximum strain shows nearly the same scale of degradation when the materials are cycled at various electric fields. After a heat treatment at 500°C for 1 h, the variations in the AFE-FE and FE-AFE phase transition due to cycling disappear, whereas the maximum strain resumes almost to its original value. Electrochemical variations are considered to contribute to the main fatigue mechanism for the material under investigation. 相似文献
67.
Inertinite concentrates from three Australian bituminous coals were hydrogenated at various temperatures ranging from 350 to 475 °C in the presence of tetralin without any added catalyst. Both conversion yields and microscopic observations of the benzene-extracted hydrogenation residues have revealed that the hydrogenation of the inertinite macerals becomes significant only > 400 °C, whereas the dissolution-hydrogenation of the accompanying vitrinite macerals occurs mainly between 350 and 400 °C. The major reaction pathway for the inertinite particles in the hydrogenation process appears to be one of initial mild carbonization followed by hydrogenation. A simplified reaction scheme is proposed which describes the reaction pathway involved in the hydrogenation of inertinite. 相似文献
68.
An innovative method for modelling biological processes under anaerobic conditions is presented and discussed. The method is based on titrimetric and off-gas measurements. Titrimetric data is recorded as the addition rate of hydroxyl ions or protons that is required to maintain pH in a bioreactor at a constant level. An off-gas analysis arrangement measures, among other things, the transfer rate of carbon dioxide. The integration of these signals results in a continuous signal which is solely related to the biological reactions. When coupled with a mathematical model of the biological reactions, the signal allows a detailed characterisation of these reactions, which would otherwise be difficult to achieve. Two applications of the method to the enhanced biological phosphorus removal processes are presented and discussed to demonstrate the principle and effectiveness of the method. 相似文献
69.
对两种煤代油方式——水煤浆和煤制混合煤气进行了燃料价格、设备投资及运行费用的分析对比。结果表明,两种代油方法均有可观的经济效益,燃料费用可分别节约52.4%和45.2%。如果原燃油工业炉窑由于工艺需要已装有除尘设备而又可用产生的蒸汽做雾化剂时,则水煤浆代油更具有经济优势。 相似文献
70.
一、概况南平市种猪场位于延平区浆甲村 ,距市区 13km ,地处 316国道旁 ,面临沙溪口库湾边。年存栏猪760 0头 ,其中母猪 60 0头 ,后备种猪 2 5 0 0头 ,商品猪 4 5 0 0头 ,是闽北集种猪、商品猪生产及科研繁育于一体的副食品基地之一。该场日排猪粪便污水约140t,未建沼气工程前 ,直接排入水库 ,严重污染水体。1999年 ,种猪场采用厌氧发酵技术 ,处理猪粪便污水 ,兴建 4× 2 0 0m3沼气工程 ,技术落后 ,基本属于农村户用水压式沼气池的简单放大 ,猪粪便污水直接进入厌氧发酵池 ,出水直接排入水库。工程设计的主要问题是 :1 不设前处理装置 ,冲洗… 相似文献