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排序方式: 共有1058条查询结果,搜索用时 15 毫秒
41.
Sarah Gilgunn Keefe Murphy Henning Stckmann Paul J. Conroy T. Brendan Murphy R. William Watson Richard J. OKennedy Pauline M. Rudd Radka Saldova 《International journal of molecular sciences》2020,21(23)
The diagnosis and treatment of prostate cancer (PCa) is a major health-care concern worldwide. This cancer can manifest itself in many distinct forms and the transition from clinically indolent PCa to the more invasive aggressive form remains poorly understood. It is now universally accepted that glycan expression patterns change with the cellular modifications that accompany the onset of tumorigenesis. The aim of this study was to investigate if differential glycosylation patterns could distinguish between indolent, significant, and aggressive PCa. Whole serum N-glycan profiling was carried out on 117 prostate cancer patients’ serum using our automated, high-throughput analysis platform for glycan-profiling which utilizes ultra-performance liquid chromatography (UPLC) to obtain high resolution separation of N-linked glycans released from the serum glycoproteins. We observed increases in hybrid, oligomannose, and biantennary digalactosylated monosialylated glycans (M5A1G1S1, M8, and A2G2S1), bisecting glycans (A2B, A2(6)BG1) and monoantennary glycans (A1), and decreases in triantennary trigalactosylated trisialylated glycans with and without core fucose (A3G3S3 and FA3G3S3) with PCa progression from indolent through significant and aggressive disease. These changes give us an insight into the disease pathogenesis and identify potential biomarkers for monitoring the PCa progression, however these need further confirmation studies. 相似文献
42.
Mikhail GelferRoland H Horst H.Henning Winter Amy M HeintzShaw Ling Hsu 《Polymer》2003,44(8):2363-2371
The effect of molecular architecture on the evolution of viscoelastic properties during crystallization was investigated using ethylene-hexene copolymers manufactured via metallocene (M-LLDPE) and Ziegler-Natta (ZN-LLDPE) processes. Differences in branching distribution were shown to have a drastic effect on the viscoelastic properties near the gel point. It is shown that the branching distribution rather than branch content is the determining parameter for the evolution of the rheological properties during isothermal and non-isothermal crystallization, and for the width of the solidification interval. We developed a partial melting technique for the preparation of stable critical gels of LLDPE whose viscoelastic properties correspond to the intermediate state between melt and solid. Local molecular conformation and crystallinity in these gels were characterized by Raman spectroscopy, which shows that the transition from melt-like to solid-like rheological behavior (physical gelation) in LLDPE occurs at a very low overall crystallinity of less than 5%. 相似文献
43.
Cover Feature: Directed Evolution of Hyaluronic Acid Synthase from Pasteurella multocida towards High‐Molecular‐Weight Hyaluronic Acid (ChemBioChem 13/2018) 下载免费PDF全文
44.
Advanced Mutasynthesis Studies on the Natural α‐Pyrone Antibiotic Myxopyronin from Myxococcus fulvus 下载免费PDF全文
J. Henning Sahner Hilda Sucipto Dr. Silke C. Wenzel Dr. Matthias Groh Prof. Dr. Rolf W. Hartmann Prof. Dr. Rolf Müller 《Chembiochem : a European journal of chemical biology》2015,16(6):946-953
Myxopyronin is a natural α‐pyrone antibiotic from the soil bacterium Myxococcus fulvus Mx f50. Myxopyronin inhibits bacterial RNA polymerase (RNAP) by binding to a part of the enzyme not targeted by the clinically used rifamycins. This mode of action makes myxopyronins promising molecules for the development of novel broad‐spectrum antibacterials. We describe the derivatization of myxopyronins by an advanced mutasynthesis approach as a first step towards this goal. Site‐directed mutagenesis of the biosynthetic machinery was used to block myxopyronin biosynthesis at different stages. The resulting mutants were fed with diverse precursors that mimic the biosynthetic intermediates to restore production. Mutasynthon incorporation and production of novel myxopyronin derivatives were analyzed by HPLC‐MS/MS. This work sets the stage for accessing numerous myxopyronin derivatives, thus significantly expanding the chemical space of f α‐pyrone antibiotics. 相似文献
45.
Andreas Winkel Wibke Dempwolf Eva Gellermann Magdalena Sluszniak Sebastian Grade Wieland Heuer Michael Eisenburger Henning Menzel Meike Stiesch 《International journal of molecular sciences》2015,16(2):4327-4342
Peri-implant infections from bacterial biofilms on artificial surfaces are a common threat to all medical implants. They are a handicap for the patient and can lead to implant failure or even life-threatening complications. New implant surfaces have to be developed to reduce biofilm formation and to improve the long-term prognosis of medical implants. The aim of this study was (1) to develop a new method to test the antibacterial efficacy of implant surfaces by direct surface contact and (2) to elucidate whether an innovative antimicrobial copolymer coating of 4-vinyl-N-hexylpyridinium bromide and dimethyl(2-methacryloyloxyethyl) phosphonate (VP:DMMEP 30:70) on titanium is able to reduce the attachment of bacteria prevalent in peri-implant infections. With a new in vitro model with semi-coated titanium discs, we were able to show a dramatic reduction in the adhesion of various pathogenic bacteria (Streptococcus sanguinis, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis), completely independently of effects caused by soluble materials. In contrast, soft tissue cells (human gingival or dermis fibroblasts) were less affected by the same coating, despite a moderate reduction in initial adhesion of gingival fibroblasts. These data confirm the hypothesis that VP:DMMEP 30:70 is a promising antibacterial copolymer that may be of use in several clinical applications. 相似文献
46.
Lipid‐like Peptides can Stabilize Integral Membrane Proteins for Biophysical and Structural Studies 下载免费PDF全文
Katharina Veith Maria Martinez Molledo Yasser Almeida Hernandez Dr. Inokentijs Josts Julius Nitsche Dr. Christian Löw Prof. Dr. Henning Tidow 《Chembiochem : a European journal of chemical biology》2017,18(17):1735-1742
A crucial bottleneck in membrane protein structural biology is the difficulty in identifying a detergent that can maintain the stability and functionality of integral membrane proteins (IMPs). Detergents are poor membrane mimics, and their common use in membrane protein crystallography may be one reason for the challenges in obtaining high‐resolution crystal structures of many IMP families. Lipid‐like peptides (LLPs) have detergent‐like properties and have been proposed as alternatives for the solubilization of G protein‐coupled receptors and other membrane proteins. Here, we systematically analyzed the stabilizing effect of LLPs on integral membrane proteins of different families. We found that LLPs could significantly stabilize detergent‐solubilized IMPs in vitro. This stabilizing effect depended on the chemical nature of the LLP and the intrinsic stability of a particular IMP in the detergent. Our results suggest that screening a subset of LLPs is sufficient to stabilize a particular IMP, which can have a substantial impact on the crystallization and quality of the crystal. 相似文献
47.
A cost‐effective and robust encapsulation system for chemical admixtures with delayed release mechanisms for construction materials like dry mix mortars is presented. Based on supplementary cementitious materials, a superplasticizer was encapsulated in matrix‐based encapsulations. Subsequently, the particle characteristics of the agglomerates and the release behavior of the superplasticizer were examined. The main objective of this research was to prove the functionality of such encapsulations for the future use in construction materials. The results obtained indicate that the performance of construction chemicals could be improved by encapsulation and controlled release for the future development of new construction materials. Furthermore, material characteristics of the supplementary cementitious material like the incline to excessive dusting or the materials density were improved by encapsulation. 相似文献
48.
Dr. Nikolay B. Anikin Prof. Dr. Rainer Suntz Prof. Dr.‐Ing. Henning Bockhorn 《化学,工程师,技术》2013,85(5):696-704
A recently developed and patented fast tomographic reconstruction system has been applied to detect two‐dimensional distributions of the OH* chemiluminescence in laminar and turbulent flames. This could be achieved by the arrangement at exposure times down to 100 – 200 μs at a spatial resolution of < 1 – 2 mm, even though the concentration of OH* in flames is in the order of some ppb and, therefore, extremely small. Among other things the results provide important information regarding the determination of the heat release rate in flames to predict unstable operating points of gas turbines. 相似文献
49.
Dr. Steffen Waglöhner Dr. Dirk Reichert Prof. Henning Bockhorn Prof. Sven Kureti 《化学,工程师,技术》2013,85(5):686-695
Three commercial carbon black samples as well as self‐made C3H6 soot were investigated for their reactivity in the oxidation on an α‐Fe2O3 catalyst. These studies were performed by temperature programmed oxidation (TPO) using a packed bed. For reference purposes, TPO studies in the absence of the catalyst were made as well. The carbon black samples were characterized towards the content of C, H, N and O as well as higher heating value, specific surface area, moisture and volatile matter and were deemed to be suitable model substances for diesel soot of different maturity. The correlation of these physico‐chemical properties with the kinetics in catalytic TPO indicated that the soot oxidation on Fe2O3 is significantly affected by the initial number of surface oxygen compounds of the soot. The decomposition of these surface species causes the formation of active carbon sites, which are supposed to accelerate the soot oxidation. 相似文献
50.
Random or tapered solution styrene–butadiene copolymer (SSBR) is very difficult to prepare in an isothermal batch process without the use of polar modifiers because of the diverse reactivity ratios of the styrene and the butadiene in hydrocarbon solvents. In the presence of polar modifiers, the random SSBR can be synthesized by anionic living polymerization with the variety of microstructures, which results in the change of glass transition temperature (Tg). This article will discuss the use of sodium dodecylbenzene sulfonate as a polar modifier in isothermal batch process that controls the microstructure of the SSBR resulting in a random as well as tapered SSBR with low Tg (?67°C to ?80°C). The Tg of SSBR was controlled by the styrene content rather than the microstructure of polybutadiene. Physical properties of SSBR compounding were discussed for tire tread applications. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013 相似文献