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排序方式: 共有2115条查询结果,搜索用时 15 毫秒
31.
Hideaki Yamada Akiyoshi Chayahara Yoshiaki Mokuno Shin-ichi Shikata 《Diamond and Related Materials》2008,17(4-5):494-497
A new structure of microwave plasma for chemical vapor deposition of diamond crystal is proposed. The structure is designed numerically, for which an improved model given in our previous work [H. Yamada et al., J. Appl. Phys. 101 (2007), art. no. 063302.] is utilized. The experimental observations and numerical predictions agree well with each other. It is demonstrated experimentally that the proposed structure can achieve a growth rate larger than 50 μm/h over an area 1 in. in diameter. 相似文献
32.
E. Nakayama T. Uchida I. Souma T. Tomita Z. H. Chen A. Murayama Y. Oka 《Journal of Superconductivity and Novel Magnetism》2003,16(2):407-410
We have studied effects of the digital-doping profile of MnSe layers on the giant magneto-optical properties in Zn1?x Mn x Se-based quantum wells. The giant Zeeman shift energy increases monotonically with increasing spatial overlap of the exciton wavefunction with the 0.5 monatomic-thick effective Mn layers at the interfaces between the digitally doped MnSe layers and nonmagnetic ZnSe layers. Also, a field-induced enhancement factor of the excitonic photoluminescence intensity, because of the suppression of the exciton energy transfer into the d-d transition of Mn-ions, increases linearly with increasing such overlap of the exciton wavefunction with the effective Mn layer. In addition, the formation energy as large as 18.6 meV and the formation time of the magnetic polaron are determined, which are also affected by the digital-doping profile. 相似文献
33.
A Kuniyasu K Itagaki T Shibano M Iino G Kraft A Schwartz H Nakayama 《Canadian Metallurgical Quarterly》1998,273(8):4635-4641
To identify the binding domain of a new Ca2+ antagonist semotiadil on L-type Ca2+ channels from skeletal muscle, photolabeling was carried out by using an azidophenyl derivative of [3H]semotiadil. Photoincorporation was observed in several polypeptides of membrane triad preparations; the only specific photoincorporation was in the alpha1 subunit of the Ca2+ channel. After solubilization and purification, the photolabeled alpha1 subunit was subjected to proteolytic and CNBr cleavage followed by antibody mapping. Specific labeling was associated solely with the region of transmembrane segment S6 in repeat IV. Quantitative immunoprecipitation was found in the tryptic and the Lys-C/Glu-C fragments of 6.6 and 6.1 kDa, respectively. Further CNBr cleavage of the Lys-C digests produced two smaller fragments of 3.4 and 1.8 kDa that were included in the tryptic and Lys-C/Glu-C fragments. The smallest labeled fragments were: Tyr1350-Met1366 and Leu1367-Met1381 containing IVS6, a possible pore-forming region. The data suggest that semotiadil binds to a region that is overlapped with but not identical to those for phenylalkylamines, dihydropyridines and benzothiazepines. The present study also provides evidence that region IV represents an important component of a binding pocket for Ca2+ antagonists. 相似文献
34.
35.
M Fujimoto H Kanzaki H Nakayama T Higuchi H Hatayama M Iwai Y Kaneko T Mori J Fujita 《Canadian Metallurgical Quarterly》1996,137(3):1096-1101
Differentiation of endometrial stromal cells (decidualization) is essential for embryo implantation and maintenance of pregnancy. By sequential complementary DNA subtractive hybridization, one of the messenger RNAs (mRNA) induced by progesterone in human endometrial stromal cells decidualized in vitro was identified as that of a tissue transglutaminase type II (TGase). TGase mRNA was induced within 6 h after the addition of progesterone to the culture, and the effect was dose dependent. Both the TGase inhibitor monodansylcadaverine and oligodeoxynucleotide complementary to the TGase mRNA inhibited the decidualization, as assessed by PRL production and morphological transformation. Expression of TGase mRNA in human decidua and endometria exposed to high levels of progesterone in vivo was demonstrated by Northern blotting and in situ hybridization. These data suggest that TGase is necessary for the decidualization of human endometrial stromal cells and that clarification of the mechanism of action of TGase will facilitate further insight into the diagnosis and treatment of infertility. 相似文献
36.
A Hamasaki F Sendo K Nakayama N Ishida I Negishi K Nakayama S Hatakeyama 《Canadian Metallurgical Quarterly》1998,188(11):1985-1992
To elucidate the role of A1, a new member of the Bcl-2 family of apoptosis regulators active in hematopoietic cell apoptosis, we established mice lacking A1-a, a subtype of the A1 gene in mice (A1-a-/- mice). Spontaneous apoptosis of peripheral blood neutrophils of A1-a-/- mice was enhanced compared with that of either wild-type mice or heterozygous mutants (A1-a+/- mice). Neutrophil apoptosis inhibition induced by lipopolysaccharide treatment in vitro or transendothelial migration in vivo observed in wild-type mice was abolished in both A1-a-/- and A1-a+/- animals. On the other hand, the extent of tumor necrosis factor alpha-induced acceleration of neutrophil apoptosis did not differ among A1-a-/-, A1-a+/-, and wild-type mice. The descending order of A1 mRNA expression was wild-type, A1-a+/-, and A1-a-/-. Taken together, these results suggest that A1 is involved in inhibition of certain types of neutrophil apoptosis. 相似文献
37.
Kajihara Akiro; Komooka Hitoshi; Kamiya Kenshu; Umeyama Hideaki 《Protein engineering, design & selection : PEDS》1993,6(6):615-620
Bovine pancreatic /S-trypsin (PDB ID-code: 1TPO) which is registeredin the Brookhaven Protein Data Bank (PDB) consists of four exons.The results of homology searches for each exon in the PDB showedthat homologous proteins were tonin (PDB ID-code: 1TON), ratmast cell protease (PDB ID-code: 3RP2_A), kaffikrein A (PDBID-code: 2PKA_B) and kallikrein A (2PKA_B) respectively. Thus,for the three-dimensional structure prediction of 1TPO, a chimeraprotein was constructed from the three proteins mentioned aboveand the 3-D structure prediction was performed using this chimerareference protein. The modelled structure of 1TPO was energeticallyoptimized by molecular mechanics and molecular dynamics simulationand was compared with its X-ray crystal structure registeredin the PDB. The root mean square deviations (r.m.s.d.) of mainchain atoms and the neighbouring active site (5 sphere fromHis57, AsplO2 and Serl95) between the modelled structure andthe X-ray structure were 1.66 and 0.94 respectively. Porcinepancreatic elastase (PDB ID-code: 3EST) which is registeredin the PDB was used as the reference protein and the modelledstructure from 3EST was also compared with the X-ray data. Ther.m.s.d. of main chain atoms and that of the active site were2.14 and 1.18 respectively. These results dearly support thepropriety of this method using the chimera reference protein. 相似文献
38.
Hideaki Itoh Shigeharu Naka Tsuneaki Matsudaira Hiroshi Hamamoto 《Journal of Materials Science》1990,25(1):533-536
A sintered compact of titanium diboride (TiB2) was prepared by hot pressing of the synthesized TiB2 powder, which was obtained by a solid-state reaction between TiN and amorphous boron. Densification of the sintered compact occurred at 20 MPa and 1800° C for 5 to 60 min with the aid of a reaction sintering, including the TiB2 formation reaction between excess 20 at % amorphous boron in the as-synthesized powder (TiB2 + 0.2B) and intentionally added 10 at % titanium metal. A homogeneous sintered compact of a single phase of TiB2, which was prepared by hot pressing for 30 min from the starting powder composition [(TiB2 + 0.2B) + 0.1 Ti], had a fine-grained microstructure composed of TiB2 grains with diameters of 2 to 3 m. The bulk density was 4.47 g cm–3, i.e. 98% of the theoretical density. The microhardness, transverse rupture strength and fracture toughness of the TiB2 sintered compact were 2850 kg mm–2, 48 kg mm–2 and 2.4 MN m–3/2, respectively. The thermal expansion coefficient increased with increasing temperature up to 400° C and had a constant value of 8.8 x 10–6 deg–1 above 500° C. 相似文献
39.
Hideaki Itoh Tsuneaki Matsudaira Shigeharu Naka Hiroshi Hamamoto 《Journal of Materials Science》1989,24(2):420-424
TiB2 powder was synthesized by a controlled formation reaction from titanium metal and amorphous boron. Precursory TiB2 formed by the pretreatment of the mixed powder (mole ratio: B/Ti=2.0) at 600° C for 60 min in an argon stream. Hollow TiB2 powder with an average grain size of 15m was obtained by subsequent heat treatment above 900° C for more than 60 min in an argon stream. The formation reaction of TiB2 powder was further controlled by pretreatment of the mixed powder at 600° C for 60 min in a hydrogen and argon stream and subsequent heat treatment at 1000° C for 360 min in an argon stream, when hollow-free TiB2 powder was formed by a milder formation reaction between amorphous boron and the reformed titanium metal with hydrogen diffused lattice. 相似文献
40.
Natsuhiko Kuratomi Shinichi Takano Mitsuharu Fukasawa Shinya Maekawa Makoto Kadokura Hiroko Shindo Ei Takahashi Sumio Hirose Yoshimitsu Fukasawa Satoshi Kawakami Hiroshi Hayakawa Hitomi Takada Natsuko Nakakuki Ryoh Kato Tatsuya Yamaguchi Yasuhiro Nakayama Hiromichi Kawaida Hiroshi Kono Taisuke Inoue Tetsuo Kondo Daisuke Ichikawa Nobuyuki Enomoto 《International journal of molecular sciences》2021,22(6)
New biomarkers are needed to further stratify the risk of malignancy in intraductal papillary mucinous neoplasm (IPMN). Although microRNAs (miRNAs) are expected to be stable biomarkers, they can vary owing to a lack of definite internal controls. To identify universal biomarkers for invasive IPMN, we performed miRNA sequencing using tumor-normal paired samples. A total of 19 resected tissues and 13 pancreatic juice samples from 32 IPMN patients were analyzed for miRNA expression by next-generation sequencing with a two-step normalization of miRNA sequence data. The miRNAs involved in IPMN associated with invasive carcinoma were identified from this tissue analysis and further verified with the pancreatic juice samples. From the tumor-normal paired tissue analysis of the expression levels of 2792 miRNAs, 20 upregulated and 17 downregulated miRNAs were identified. In IPMN associated with invasive carcinoma (INV), miR-10a-5p and miR-221-3p were upregulated and miR-148a-3p was downregulated when compared with noninvasive IPMN. When these findings were further validated with pancreatic juice samples, miR-10a-5p was found to be elevated in INV (p = 0.002). Therefore, three differentially expressed miRNAs were identified in tissues with INV, and the expression of miR-10a-5p was also elevated in pancreatic juice samples with INV. MiR-10a-5p is a promising additional biomarker for invasive IPMN. 相似文献