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排序方式: 共有678条查询结果,搜索用时 15 毫秒
41.
Tominaga J Michizoe J Kamiya N Ichinose H Maruyama T Goto M 《Journal of Bioscience and Bioengineering》2004,98(1):14-19
Catalytic oxidation of biphenyl derivatives was investigated using laccase in a homogeneous aqueous-organic system. A thermostable laccase from Trametes sp. showed the highest catalytic activity for the oxidation of 4-hydroxybiphenyl (4-HB) at a reaction temperature of 60 degrees C when dimethylsulfoxide (DMSO) was employed as a co-solvent. Furthermore, the catalytic performance was successfully enhanced by the incorporation of a laccase mediator system (LMS) into the aqueous-DMSO media. The catalytic performance strongly depended on the type of mediator, and the highest activity was observed with 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) as mediator, suggesting the importance of the selection of a suitable mediator. It was verified that this mediator system is applicable to the oxidation of several biphenyl derivatives with hydroxyl groups. 相似文献
42.
In order to develop a short-term algal toxicity test, the growth of and the phosphate uptake by the green alga Selenastrum capricornutum during batch culture were observed. In the control medium, S. capricornutum took up phosphate earlier than it grew. It was also observed that the phosphate uptake was inhibited by the presence of a toxicant. From these results, phosphate uptake was considered as one of the useful effect parameters for a short-term algal toxicity test. As the removal rate of phosphate from the medium is a function of the amount of algal cell initially inoculated, the test period is variable. The relationship between the amount of inoculation and phosphate uptake was examined and the test conditions suitable for a 3-h toxicity test were established as one example. According to this test procedure, the inhibitory effect of some toxicants on the phosphate uptake was determined. For comparison, a conventional algal assay based on algal growth was also performed. The EC50s for both tests were close. This indicated that the algal toxicity test method proposed in this paper would be useful for the uses where rapidity is required. 相似文献
43.
44.
An improvement of Fourier synthesis optics for hard x-ray imaging is described, and the basic performance of the new optics is confirmed through numerical simulations. The original concept of the Fourier synthesis imager utilizes nonposition-sensitive hard x-ray detectors coupled to individual bigrid modulation collimators. The improved concept employs a one-dimensional position-sensitive detector (such as a CdTe strip detector) instead of the second grid layer of each bigrid modulation collimator. This improves the imaging performance in several respects over the original design. One performance improvement is a two-fold increase in the average transmission, from 1/4 to 1/2. The second merit is that both the sine and cosine components can be derived from a single grid-detector module, and hence the number of imaging modules can be halved. Furthermore, it provides information along the depth direction simultaneously. This in turn enables a three-dimensional imaging hard x-ray microscope for medical diagnostics, incorporating radioactive tracers. A conceptual design of such a microscope is presented, designed to provide a field of view of 4 mm and a spatial resolution of 400 microm. 相似文献
45.
Amizuka N Shimomura J Li M Seki Y Oda K Henderson JE Mizuno A Ozawa H Maeda T 《Journal of electron microscopy》2003,52(6):503-513
Previous studies have reported enhanced osteoclastogenesis, increased bone resorption and osteoporosis in osteoprotegerin (OPG)-deficient mice. In the present study, we show that the tibial epiphyses contain abundant, thin trabeculae lined with numerous osteoclasts and cuboidal osteoblasts. The increase in osteoblasts and osteoclasts was associated with a dramatic increase in calcein labelling of the mineralization fronts and replacement of much of the intertrabecular marrow with numerous alkaline phosphatase-positive preosteoblasts. Furthermore, the discrete, linear cement lines seen in wild-type mice were replaced by a randomly oriented meshwork of cement lines that were stained intensely for tartrate-resistant acid phosphatase and osteopontin in the OPG-/- mice. These indices of accelerated bone remodelling in mutant bone were associated with irregular trabecular surfaces, a disorganized collagen matrix interspersed with amorphous ground substance and numerous fissures between old and new bone. In total, these observations indicate that enhanced osteoclastic activity in OPG-/- epiphyses led to a coupled increase in osteoblast differentiation and activity and an increase in bone remodelling. The high bone turnover, disorganized matrix and impaired attachment of new to old bone in the cement lines in OPG-/- mice appear to cause bone fragility. 相似文献
46.
Takashi Kojima Yasuki Mori Masahiro Kamiya Ryo Sasai Hideaki Itoh 《Journal of Materials Science》2007,42(15):6056-6061
Capability of the recycling of high strength and high fracture toughness yttria-stabilized tetragonal zirconia polycrystalline
(Y-TZP) sintered body utilizing “low-temperature annealing degradation” phenomenon was investigated. Hydrothermal treatment
was employed to induce the phase transformation from tetragonal to monoclinic zirconia and to disintegrate the Y-TZP sintered
body. 3 mol% Y2O3–ZrO2 specimens sintered at 1,550 °C and more were disintegrated without leaving the original appearances when the treatment temperature
was between 200 °C and 400 °C. The size of the disintegrated fragments of Y-TZP sintered body was much affected by hydrothermal
treatment conditions. Only with hydrothermal treatment and simple ball milling, the sintered body was pulverized into the
primary particle level. This technique is expected to apply to a sustainable recycling system for the zirconia ceramics, which
restrains an energy consumption compared to crushing zirconia using mechanical procedures. 相似文献
47.
Kyohei Terao Chihiro Masuda Ryo Inukai Murat Gel Hidehiro Oana Masao Washizu Takaaki Suzuki Hidekuni Takao Fusao Shimokawa Fumikazu Oohira 《IET nanobiotechnology / IET》2016,10(3):124
Optical tweezers are powerful tools for manipulating single DNA molecules using fluorescence microscopy, particularly in nanotechnology‐based DNA analysis. We previously proposed a manipulation technique using microstructures driven by optical tweezers that allows the handling of single giant DNA molecules of millimetre length that cannot be manipulated by conventional techniques. To further develop this technique, the authors characterised the microstructures quantitatively from the view point of fabrication and efficiency of DNA manipulation under a fluorescence microscope. The success rate and precision of the fabrications were evaluated. The results indicate that the microstructures are obtained in an aqueous solution with a precision ∼50 nm at concentrations in the order of 106 particles/ml. The visibility of these microstructures under a fluorescence microscope was also characterised, along with the elucidation of the fabrication parameters needed to fine tune visibility. Manipulating yeast chromosomal DNA molecules with the microstructures illustrated the relationship between the efficiency of manipulation and the geometrical shape of the microstructure. This report provides the guidelines for designing microstructures used in single DNA molecule analysis based on on‐site DNA manipulation, and is expected to broaden the applications of this technique in the future.Inspec keywords: DNA, molecular biophysics, fluorescence, optical microscopy, radiation pressure, biological techniquesOther keywords: optically driven microstructures, single DNA molecule analysis, fluorescence microscopy, optical tweezers, nanotechnology‐based DNA analysis, manipulation technique, aqueous solution, fine tune visibility, yeast chromosomal DNA molecules, geometrical shape, on‐site DNA manipulation 相似文献
48.
CdSe microcrystals were successfully dispersed in GeO2 glass andCaF2 crystal thin films by rf-sputtering. All films preparedshowed the blue shift of absorption edge in visible spectra due toquantum size effect. The amount of the blue shift of the filmsincreased with decreasing the size of the microcrystals. Comparingthe amount of the particle size–depending blue shift of CdSemicrocrystals dispersed in between the GeO2, CaF2 and SiO2 matricies, the influence of the matrix on the shift was found.Therefore, it can be said that there is an influence of the matrix onthe quantum size effect. From the modified theoretical calculation,the influence of matrix was considered to appear through Coulombinteraction between electrons and holes. 相似文献
49.
50.
This article presents a study of the effect of through-the-thickness stitching yarns upon the strength and failure behavior
of multidirectionally reinforced composites. The in-plane yarns were placed in four directions (0,±45, 90) to form a quasi-isotropic
preform, which had open spaces between adjacent yarns. These interyarn spaces allowed easy insertion of the through-the-thickness
stitching yarns without significant damage of the in-plane fibers. Fiber volume fractions of over 54 pct were obtained by
this method. The through-the-thickness yarn sizes used in this study were 2, 4, and 6 kilo-filament (kf). Non-stitched performs
were also manufactured with the same fiber content and by the same procedure as the stiched preforms for the control experiments.
All preforms were infiltrated with epoxy resin by the resin transfer molding (RTM) technique. In-plane tensile and compressive
strength, interlaminar shear strength, and mode I fracture toughness of the carbon/epoxy composites were measured at three
through-the-thickness yarn contents. Although the through-the-thickness yarns significantly enhanced the mode I fracture toughness,
they tended to degrade the in-plane tensile and compressive strength. The failure process under interlaminar shear loading
by double notch shear tests showed two distinct stages: the fiber-matrix interfacial failure followed by the breakage/debonding
of the through-the-thickness yarns. The through-the-thickness yarns caused a reduction of the initial failure load in the
first stage but could enhance the final failure load in the second stage. In composites with 6 kf through-the-thickness yarns,
the final failure load could exceed the initial failure load. Scanning electron microscope (SEM) and optical microscopic examinations
were also conducted for observing the failure mechanisms and fracture surfaces.
This article is based on a presentation made in the Symposium “Mechanisms and Mechanics of Composites Fracture” held October
11–15, 1998, at the TMS Fall Meeting in Rosemont, Illinois, under the auspices of the TMS-SMD/ASM-MSCTS Composite-Materials
Committee. 相似文献