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101.
Sea urchin shells are generally discarded as food waste after removal of edible gonads, although they possess significant amount of polyhydroxylated naphtoquinone pigments having potent antioxidant property. In this study, separation and quantification of the pigment mixtures from purple sea urchin shells were performed using a C18 reversed-phase HPLC with isocratic elution of 50% mixture of solvent (A) formic acid: water (0.1: 100, v/v) and solvent (B) MeOH: acetonitrile (5: 9, v/v) at a flow rate of 0.5 mL/min. The pigment mixtures were separated into four major peaks in 30 min, which assumed to be spinochromes A, B and C and echinochrome A on the basis of UV-Vis absorption spectra obtained by a diode array detector. LC-MS analysis also supported these results by producing the predominant base peaks at m/z 221.1, 279.1, 265.1, and 263.1 corresponding to the pseudomolecular ions [M-H]− of the respective compounds. The HPLC method also showed good correlations (r2 > 0.98) between injected amounts of these pigments and peak areas recorded at 340 and 520 nm. These results indicated that the HPLC method developed in this study would be applicable for the separation and quantification of these major pigments in sea urchin shells. 相似文献
102.
Dr. Hannes M. Beyer Dr. Hideo Iwaï 《Chembiochem : a European journal of chemical biology》2019,20(15):1933-1938
Protein-splicing domains are frequently used engineering tools that find application in the in vivo and in vitro ligation of protein domains. Directed evolution is among the most promising technologies used to advance this technology. However, the available screening systems for protein-splicing activity are associated with bottlenecks such as the selection of pseudo-positive clones arising from off-pathway reaction products or fragment complementation. Herein, we report a stringent screening method for protein-splicing activity in cis and trans, that exclusively selects productively splicing domains. By fusing splicing domains to an intrinsically disordered region of the antidote from the Escherichia coli CcdA/CcdB type II toxin/antitoxin system, we linked protein splicing to cell survival. The screen allows selecting novel cis- and trans-splicing inteins catalyzing productive highly efficient protein splicing, for example, from directed-evolution approaches or the natural intein sequence space. 相似文献
103.
Hideo Yamauchi Junichi Ikejiri Fumio Sato Hiroyuki Oshita Tsuyoshi Honma Takayuki Komatsu 《Journal of the American Ceramic Society》2019,102(11):6658-6667
In recent years, the expansion of demand for lithium ion batteries has resulted in soaring prices of the constituent resources. From the viewpoint of safety, studies on all-solid-state batteries are actively being carried out. In this study, we succeeded in driving all-solid-state batteries derived from nontoxic oxide glasses at room temperature without requiring scarce resources such as lithium and cobalt. The main structure of the ceramic batteries with a simple structure in which Na2FeP2O7 crystallized glass and β″-alumina solid solution are joined by pressureless cofiring at 550°C. During the crystallization of Na2O-Fe2O3-P2O5 glass, fusion with the β″-alumina solid solution is achieved. Reversible charge and discharge of 80 mAh/g were achieved at room temperature. It is not necessary to apply pressure during cell preparation or the use of the batteries. Furthermore, the strong junction at the cathode and electrolyte interface does not peel off during charge and discharge over a long period of 623 cycles. Ex situ X-ray photoelectron spectroscopy revealed partial Fe4+ induction and a reversible charge and discharge reaction even after overcharging to 9 V. It was demonstrated that Na2FeP2O7 is very stable against overcharging to 9 V. 相似文献
104.
Naoya Kanbayashi Arisa Yamazawa Koichiro Takii Taka‐aki Okamura Kiyotaka Onitsuka 《Advanced Synthesis \u0026amp; Catalysis》2016,358(4):555-560
We report the asymmetric allylic alkylation of allylic chlorides with silyl enolates as a carbon nucleophile using a planar‐chiral cyclopentadienyl‐ruthenium (Cp′Ru) catalyst. The reaction proceeds under unusually mild conditions to give the desired branched products with complete regioselectivity and high enantioselectivity, and reactive functional groups, such as aldehyde, can be tolerated. In this reaction system, Cp′Ru plays an important role in activating both silyl enolate and allylic chloride.
105.
106.
Satoshi Miyaguchi Shinichi Ishizuka Takeo Wakimoto Jun Funaki Yoshinori Fukuda Hirofumi Kubota Kenji Yoshida Teruichi Watanabe Hideo Ochi Tsuyoshi Sakamoto Masami Tsuchida Isamu Ohshita Teruo Tohma 《Journal of the Society for Information Display》1999,7(3):221-226
Abstract— A full-color 5.2-inch 1/4-VGA passive-matrix organic LED display has been developed, adopting selective deposition for the different emitting materials. The display features 320 (×3) × 240 pixels with an equivalent pixel size of 0.33 × 0.33 mm, white peak luminance of over 150 cd/m2, and power consumption of 6 W. 相似文献
107.
Hongo Takero Arakawa Hideo Sugimoto Gunji Tange Koichi Yamamoto Yuzo 《Industrial Electronics, IEEE Transactions on》1987,(1):5-10
An automatically guided vehicle, traveling without fixed guide ways, has been developed. In this paper, the construction of the vehicle, the control algorithm, and its general performance are described. 相似文献
108.
109.
Michio Niwano Hideo Kitamura 《Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment》1985,234(2):388-393
A new type of undulator greatly improved in tunability as compared with a usual undulator, is proposed. The undulator has a composite magnetic field which is a linear superposition of two sinusoidally varying fields with different spatial periods. The wavelength can be changed by varying the field strength of the longer-period component while keeping that of the shorter-period component at the highest possible value. Numerical calculations of the spectral brightness were carried out. The proper ratios between the two spatial periods were found to be 3:1, 5:1 and 7:1. 相似文献
110.
Shuyan Gao Naoto Koshizaki Hideo Tokuhisa Emiko Koyama Takeshi Sasaki Jae‐Kwan Kim Joonghyun Ryu Deok‐Soo Kim Yoshiki Shimizu 《Advanced functional materials》2010,20(1):78-86
Colloidal Au‐amplified surface plasmon resonance (SPR), like traditional SPR, is typically used to detect binding events on a thin noble metal film. The two major concerns in developing colloidal Au‐amplified SPR lie in 1) the instability, manifested as a change in morphology following immersion in organic solvents and aqueous solutions, and 2) the uncontrollable interparticle distance, determining probe spacing and inducing steric hindrance between neighboring probe molecules. This may introduce uncertainties into such detecting techniques, degrade the sensitivity, and become the barricade hampering colloidal Au‐based transducers from applications in sensing. In this paper, colloidal Au‐amplified SPR transducers are produced by using ultrathin Au/Al2O3 nanocomposite films via a radio frequency magnetron co‐sputtering method. Deposited Au/Al2O3 nanocomposite films exhibit superior stability, and average interparticle distances between Au nanoparticles with similar average sizes can be tuned by changing surface coverage. These characteristics are ascribed to the spacer function and rim confinement of dielectric Al2O3 and highlight their advantages for application in optimal nanoparticle‐amplified SPR, especially when the probe size is smaller than the target molecule size. This importance is demonstrated here for the binding of protein (streptavidin) targets to the probe (biotin) surface. In this case, the dielectric matrix Al2O3 is a main contributor, behaving as a spacer, tuning the concentration of Au nanoparticles, and manipulating the average interparticle distance, and thus guaranteeing an appropriate number of biotin molecules and expected near‐field coupling to obtain optimal sensing performance. 相似文献