SecA2 Associates with Translating Ribosomes and Contributes to the Secretion of Potent IFN-β Inducing RNAs

Protein secretion plays a central role in modulating interactions of the human pathogen Listeria monocytogenes with its environment. Recently, secretion of RNA has emerged as an important strategy used by the pathogen to manipulate the host cell response to its advantage. In general, the Sec-dependent translocation pathway is a major route for protein secretion in L. monocytogenes, but mechanistic insights into the secretion of RNA by these pathways are lacking. Apart from the classical SecA1 secretion pathway, L. monocytogenes also encodes for a SecA paralogue (SecA2) which targets the export of a specific subset of proteins, some of which are involved in virulence. Here, we demonstrated that SecA2 co-sediments with translating ribosomes and provided evidence that it associates with a subset of secreted small non-coding RNAs (sRNAs) that induce high levels of IFN-β response in host cells. We found that enolase, which is translocated by a SecA2-dependent mechanism, binds to several sRNAs, suggesting a pathway by which sRNAs are targeted to the supernatant of L. monocytogenes.     

A dual-band semi-circular patch antenna for WiMAX and WiFi-5/6 applications

A semi-circular patch antenna (SCPA) is designed for WiMAX and WiFi-5/6 communications. The footprint of the proposed SCPA is only 30 × 40 × 1.575 mm³, which is composed of a semi-circular patch and a partial ground plane. The main strength of this work is that the estimated wide dual-frequency span of 2.39–3.75 GHz and 5.39–7.18 GHz are contributed with two sharp resonances at 2.77 and 6.46 GHz, respectively. The proposed SCPA has been practically tested after fabrication. The measured results confirm that the designed antenna achieved the bandwidth necessity for WiMAX 1.5 (2.5–2.69 GHz), WiMAX 2 (3.4–3.6 GHz), WiFi-5 (5.15–5.85 GHz) and WiFi-6 (5.925–7.125 GHz). In the two resonance frequencies of 2.77 and 6.46 GHz, the designed antenna achieved a gain of 2.558 and 4.109 dB, respectively. In addition, the SCPA also manifests good radiation properties and achieves an average efficiency of more than 90%. A professional version of the 3D electromagnetic simulator is utilized to examine the effect of diffident parameters and model the tested antenna.     

Multi-objective path planning for lung biopsy surgery

Multimedia Tools and Applications - CT image-guided lung biopsy surgery is the gold standard for lung tumor diagnosis. The preoperative path planning of the surgery is necessary, which aims to find...     

FAMCroNA: Fault Analysis in Memristive Crossbars for Neuromorphic Applications

Journal of Electronic Testing - Resistive memories have drawn the attention of researchers due to their low power and single-cycle computation of vector-matrix multiplication (VMM), which is the...     

Child–smartphone interaction: relevance and positive and negative implications

Universal Access in the Information Society - An increasing number of children are now using smartphones, and the phenomenon is being studied keenly by computer scientists, pediatricians,...     

PerSeg : segmenting salient objects from bag of single image perturbations

Multimedia Tools and Applications - Salient object segmentation is an important computer vision problem having applications in numerous areas such as video surveillance, scene parsing, autonomous...