Rapid Quantification of Live Cell Receptors Using Bioluminescence in a Flow‐Based Microfluidic Device

The number of receptors expressed by cells plays an important role in controlling cell signaling events, thus determining its behaviour, state and fate. Current methods of quantifying receptors on cells are either laborious or do not maintain the cells in their native form. Here, a method integrating highly sensitive bioluminescence, high precision microfluidics and small footprint of lensfree optics is developed to quantify cell surface receptors. This method is safe to use, less laborious, and faster than the conventional radiolabelling and near field scanning methods. It is also more sensitive than fluorescence based assays and is ideal for high throughput screening. In quantifying β₁ adrenergic receptors expressed on the surface of H9c2 cardiomyocytes, this method yields receptor numbers from 3.12 × 10⁵ to 9.36 × 10⁵ receptors/cell which are comparable with current methods. This can serve as a very good platform for rapid quantification of receptor numbers in ligand/drug binding and receptor characterization studies, which is an important part of pharmaceutical and biological research.     

Performance evaluation of heat exchanger using alternative refrigerant R407C

R22 (HCFC22) has been widely used as the refrigerant in air conditioners. According to the Montreal protocol for ozone layer protection, the total production of HCFCs has been capped since the beginning of 1996. Zeotropic refrigerant mixture R407C and nearly azeotropic refrigerant mixture R410A have been selected as alternatives to R22. We examined refrigerant passages in heat exchangers used in heat pump‐type room air conditioners using zeotropic refrigerant R407C through simulation, and obtained the following conclusions. In an indoor heat exchanger, a counter flow configuration when operating as a condenser has higher temperature efficiency. When an outdoor heat exchanger operates as an evaporator, a configuration that suppresses the temperature glide by partially reducing the refrigerant passage not only produces high efficiency, but also reduces the frost formation on fins. © 2002 Wiley Periodicals, Inc. Heat Trans Asian Res, 31(8): 626–638, 2002; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/htj.10064     

Study on heat transfer characteristics of reservoir embedded loop heat pipe (1st report,Influence of evaporator orientation against gravity and charged liquid weight on heat transfer characteristics)

High‐powered satellites need larger heat rejection areas. A deployable radiator is one of the key technologies for a high‐powered satellite bus. A Reservoir Embedded Loop Heat Pipe (RELHP) is a two‐phase heat transfer device that constitutes a deployable radiator. RELHP has an evaporator core which is used as a liquid reservoir to enhance operational reliability. This paper presents the heat transport characteristics of a RELHP under changing evaporator orientation against gravity and charged ammonia weight by experiment and calculation. Liquid slug position in the reservoir has a great influence on heat transport characteristics, caused by changing heat transfer coefficients between returned liquid into the evaporator and vapor in the reservoir. © 2007 Wiley Periodicals, Inc. Heat Trans Asian Res, 36(3): 143– 157, 2007; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/htj.20150     

Occurrence and removal of NDMA and NDMA formation potential in wastewater treatment plants

N-Nitrosodimethylamine (NDMA) is a potent carcinogen that is formed during disinfection by chlorination or ozonation in wastewater treatment plants (WWTPs). At present, little is known about the occurrence and fate of NDMA and its formation potential (FP) during wastewater treatment. We investigated the fate of NDMA and NDMA FP in 12 WWTPs. NDMA occurred in the influents at a concentration ranging from below the limit of quantification (LOQ <10 ng/L) to 80 ng/L, and in the final discharges from below the LOQ to 73 ng/L. In three WWTPs located in industrial areas, the influent had a high NDMA FP (up to 8230 ng/L). The rate of NDMA FP reduction from influent to secondary effluent varied between 85 and 98%, regardless of treatment process. The rate of NDMA removal is due more to the influent properties than to the type of biological treatment process.     

Analysis of nonlinear transient responses of piezoelectric resonators

The electric transient response method is an effective technique to evaluate material constants of piezoelectric ceramics under high-power driving. In this study, we tried to incorporate nonlinear piezoelectric behaviors in the analysis of transient responses. As a base for handling the nonlinear piezoelectric responses, we proposed an assumption that the electric displacement is proportional to the strain without phase lag, which could be described by a real and constant piezoelectric e-coefficient. Piezoelectric constitutive equations including nonlinear responses were proposed to calculate transient responses of a piezoelectric resonator. The envelopes and waveforms of current and vibration velocity in transient responses observed in some piezoelectric ceramics could be fitted with the calculation including nonlinear responses. The procedure for calculation of mechanical quality factor Q(m) for piezoelectric resonators with nonlinear behaviors was also proposed.     

An Experimental Study on Transverse Hydrogen Gas Injection into Mach 1.8 Airflow Channel-The 1st report:Single Circular Injector

INTRODUCTIONResearchonSCRamjet,whichwascarriedoutex-tensivelyillUSinthepastatthelatesixtiesandsev-ellties,hasbeenupdatedtoraisenewinterestamongstseveralcountriesintheworldbecauseoftheconcep-tualdevelopmentofaframeintegratedpropulsionsys-temforthenextgenerationtransAnatmosphereflightvehicle.However,fullcomprehensionofthebasicthermo-gasdynamicstructurewithinthecombustorisyettobeeffortedfromscientificandengineeringpointofviews,whichplaysakeyroleindevelopingthefirmtechnologyofsupersoniccombu…     

Characteristics of flamelets in spray flames formed in a laminar counterflow

A two-dimensional numerical simulation of a spray flame formed in a laminar counterflow is presented, and the flamelet characteristics are studied in detail. The effects of strain rate, equivalence ratio, and droplet size are examined in terms of mixture fraction and scalar dissipation rate. n-Decane (C₁₀H₂₂) is used as a liquid spray fuel, and the droplet motion is calculated by the Lagrangian method without the parcel model. A one-step global reaction is employed for the combustion reaction model. The results show that there appear large differences in the trends of gaseous temperature and mass fractions of chemical species in the mixture fraction space between the spray flame and the gaseous diffusion flame. The gas temperature in the spray flame is much higher than that in the gaseous diffusion flame. This is due to the much lower scalar dissipation rate and the coexistence of premixed and diffusion-limited combustion in the spray flame. For the spray flames, gas temperature and mass fractions of chemical species are not unique functions of the mixture fraction scalar dissipation rate. This is because the production rate of the mixture fraction, namely evaporation rate of the droplets, in the upstream region is not in proportion to its transport-diffusion rate in the downstream region. The behavior shows marked differences as the strain rate decreases, the equivalence ratio increases, or the droplet size decreases.     

Quantum Oscillations of Thermoelectric Effects in a Pseudo-one-dimensional Electron Gas With a Spin–Orbit Interaction

We consider thermoelectric effects in a pseudo-one-dimensional electron gas (P1DEG) with a spin–orbit interaction (SOI). The SOI splits the dispersion relation of the P1DEG into subbands with an energy gap. We find quantum oscillations in transport coefficients, which coincide with the locations of the subband edges, as a function of the electrochemical potential.     

Simultaneous analysis of heparan sulfate, chondroitin/dermatan sulfates, and hyaluronan disaccharides by glycoblotting-assisted sample preparation followed by single-step zwitter-ionic-hydrophilic interaction chromatography

Glycosaminoglycans (GAGs) play important roles in cell adhesion and growth, maintenance of extracellular matrix (ECM) integrity, and signal transduction. To fully understand the biological functions of GAGs, there is a growing need for sensitive, rapid, and quantitative analysis of GAGs. The present work describes a novel analytical technique that enables high throughput cellular/tissue glycosaminoglycomics for all three families of uronic acid-containing GAGs, hyaluronan (HA), chondroitin sulfate (CS)/dermatan sulfate (DS), and heparan sulfate (HS). A one-pot purification and labeling procedure for GAG Δ-disaccharides was established by chemo-selective ligation of disaccharides onto high density hydrazide beads (glycoblotting) and subsequent labeling by fluorescence. The 17 most common disaccharides (eight comprising HS, eight CS/DS, and one comprising HA) could be separated with a single chromatography for the first time by employing a zwitter-ionic type of hydrophilic-interaction chromatography column. These novel analytical techniques were able to precisely characterize the glycosaminoglycome in various cell types including embryonal carcinoma cells and ocular epithelial tissues (cornea, conjunctiva, and limbus).     

Spectral properties of nanoengineered Ag/Au bilayer rods fabricated by electron beam lithography

Ag/Au bimetallic nanoparticles possess the combinatory advantages of Au and Ag nanoparticles and can also be utilized to tune the properties of localized surface plasmon resonance. Ag/Au bilayer nanorods were prepared by electron beam lithography, and their spectral properties were investigated. Compared with Ag monolayer nanorods, Ag/Au bilayer nanorods show broader localized surface plasmon resonance bands, and the longitudinal mode and transverse mode localized surface plasmon bands show blueshift and redshift, respectively. The maximum near-field intensity of the longitudinal mode of the Ag/Au nanorod is less than half that of the Ag/Au nanorod without gold layer. Shape-induced modification of Ag/Au bilayer nanorods on their spectral properties was also discussed.