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61.
Quantitative detection system for maize sample containing combined-trait genetically modified maize 总被引:4,自引:0,他引:4
Akiyama H Watanabe T Wakabayashi K Nakade S Yasui S Sakata K Chiba R Spiegelhalter F Hino A Maitani T 《Analytical chemistry》2005,77(22):7421-7428
Various countries have established regulations that stipulate the labeling of agricultural commodities, feed, and food products that contain or are made from genetically modified (GM) material or that contain adventitious GM material in amounts that exceed certain threshold levels. While regulations in some countries refer to GM material on a weight per weight (w/w) percentage, the currently applied detection methods do not directly measure the w/w percentage of the GM material. Depending on the particular method and the sample matrix it is applied to, the conversion of analytical results to a w/w percentage is challenging or not possible. The first rapid PCR system for GM maize detection on a single kernel basis has been developed. The equipment for the grinding of individual kernels and a silica membrane-based 96-well DNA extraction kit were both significantly revised and optimized for this particular purpose, respectively. We developed a multiplex real-time PCR method for the rapid quantification of GM DNA sequences in the obtained DNA solutions. In addition, a multiplex qualitative PCR detection method allows for the simultaneous detection of different GM maize traits in each kernel and thereby for identification of individual kernels that contain a combination of two or more GM traits. Especially for grain samples that potentially contain combined-trait GM maize kernels, the proposed methods can deliver informative results in a rapid, precise, and reliable manner. 相似文献
62.
A Restart Control Method for Position Sensorless PMSM Drive Systems Without Potential Transformer for Railway Vehicle Traction
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Shun Taniguchi Kazuya Yasui Kazuaki Yuki Yosuke Nakazawa Shouji Onda 《Electrical Engineering in Japan》2015,193(3):44-53
This paper proposes a restart control method for position sensorless PMSM drive systems without a potential transformer for railway vehicle traction. This method can estimate the initial rotor speed and position under coasting conditions over the entire speed range. The method can also be used when the back‐EMF voltage is higher than the inverter DC link voltage. The proposed method is verified by experiments using a 200‐kW PMSM. 相似文献
63.
Dr. Dae-Shik Kim Dr. Atsushi Endo Dr. Francis G. Fang Dr. Kuan-Chun Huang Dr. Xingfeng Bao Dr. Hyeong-wook Choi Dr. Utpal Majumder Dr. Young Y. Shen Steven Mathieu Xiaojie Zhu Kristen Sanders Dr. Thomas Noland Dr. Ming-Hong Hao Dr. Yu Chen Dr. John Y. Wang So Yasui Karen TenDyke Jiayi Wu Christy Ingersoll Kara A. Loiacono Dr. Janna E. Hutz Dr. Nadeem Sarwar 《ChemMedChem》2021,16(11):1741-1744
A strategy for creating potent and pan-genotypic stimulator of interferon genes (STING) agonists is described. Locking a bioactive U-shaped conformation of cyclic dinucleotides by introducing a transannular macrocyclic bridge between the nucleic acid bases leads to a topologically novel macrocycle-bridged STING agonist (MBSA). In addition to substantially enhanced potency, the newly designed MBSAs, exemplified by clinical candidate E7766 , exhibit broad pan-genotypic activity in all major human STING variants. E7766 is shown to have potent antitumor activity with long lasting immune memory response in a mouse liver metastatic tumor model. Two complementary stereoselective synthetic routes to E7766 are also described. 相似文献
64.
Masatomo Yamagiwa Takayuki Ogawa Takeo Minamikawa Dahi Ghareab Abdelsalam Kyosuke Okabe Noriaki Tsurumachi Yasuhiro Mizutani Testuo Iwata Hirotsugu Yamamoto Takeshi Yasui 《Journal of Infrared, Millimeter and Terahertz Waves》2018,39(6):561-572
Terahertz digital holography (THz-DH) has the potential to be used for non-destructive inspection of visibly opaque soft materials due to its good immunity to optical scattering and absorption. Although previous research on full-field off-axis THz-DH has usually been performed using Fresnel diffraction reconstruction, its minimum reconstruction distance occasionally prevents a sample from being placed near a THz imager to increase the signal-to-noise ratio in the hologram. In this article, we apply the angular spectrum method (ASM) for wavefront reconstruction in full-filed off-axis THz-DH because ASM is more accurate at short reconstruction distances. We demonstrate real-time phase imaging of a visibly opaque plastic sample with a phase resolution power of λ/49 at a frame rate of 3.5 Hz in addition to real-time amplitude imaging. We also perform digital focusing of the amplitude image for the same object with a depth selectivity of 447 μm. Furthermore, 3D imaging of visibly opaque silicon objects was achieved with a depth precision of 1.7 μm. The demonstrated results indicate the high potential of the proposed method for in-line or in-process non-destructive inspection of soft materials. 相似文献
65.
Efficient High-Power Green Beam Generation by Use of an Intracavity Frequency-Doubled Laser-Diode-Pumped Q-Switched Nd:YAG Laser 总被引:4,自引:0,他引:4
An intracavity frequency-doubled diode-side-pumped Nd:YAG laser has been developed. A maximum green power of 20 W was generated at 20-kHz repetition rate with 14.2% optical-to-optical conversion efficiency and 5.3% electrical-to-optical conversion efficiency. 相似文献
66.
Fire Safety Performance of Japanese Traditional Wood/Soil Walls and Implications for the Restoration of Historic Buildings in Urban Districts 总被引:2,自引:0,他引:2
Yuji Hasemi Noboru Yasui Michitaka Akizuki Koichi Kinoshita Koichi Yamamoto Masatomo Yoshida Yoshihide Tamura Mariko Takeda 《Fire Technology》2002,38(4):391-402
Grass-root research activities to clarify fire safety performance of Japanese traditional wooden construction are introduced. The activities aim to resolve conflict between the restoration of historic buildings and the reduction of risk and hazard of urban fires. Among various activities, scope and results of fire resistance tests on traditional soil wall assemblies supported by timber frame are reported in detail. The test results indicate an encouraging prospect for the rational fire safety assessment of historic buildings and further development of fire-safe traditional constructions for the restoration of historic buildings and historic urban districts. 相似文献
67.
68.
Takeshi Yasui Tomoko Sasaki Junko Matsuki 《Journal of the science of food and agriculture》1999,79(5):687-692
Two waxy endosperm mutant lines, K107Wx1 and K107Wx2, derived from a bread wheat cultivar, Kanto 107, had a lower flour yield in test milling and a lower peak temperature in flour pasting measurement than their parent. Starch content in the kernel of waxy mutants was low, while fat and (1→3),(1→4)‐β‐D‐glucan content was high compared with cv Kanto 107, but protein content was similar for all of them. Waxy mutants had less α‐amylase activity than their non‐waxy parent, and had lower peak viscosity of flour paste in the pasting medium of water. A pasting test in silver nitrate solution increased peak viscosity in both waxy and non‐waxy flours. The viscosity of waxy flour paste rapidly increased above 60°C, while that of non‐waxy flour paste increased gradually at 60–80°C. The difference in pasting behaviour was attributed to the presence of amylose in starch granules. © 1999 Society of Chemical Industry 相似文献
69.
Large A‐type endosperm starch granules were isolated from near‐isogenic waxy and non‐waxy lines and low‐amylose mutant lines of bread wheat with a common genetic background. The amylose contents of A‐type starch ranged from 2.6% to 23.6%. Amylopectin was isolated by concanavalin A (Con A) precipitation from the isolated starch. The λmax (range: 532‐538 nm) and blue values at 680 nm (range: 0.026‐0.037) of the iodine‐amylopectin complex were not significantly different among the isolated amylopectins, indicating that amylopectins from non‐waxy and low‐amylose lines did not contain such long chains as amylose or extra‐long chains of amylopectin affecting iodine complex properties. Chain‐length distribution profiles measured by both high‐performance size‐exclusion chromatography (HPSEC) and high‐performance anion‐exchange chromatography (HPAEC) showed that the amylopectin structures of these lines were indistinguishable from each other. Extra‐long chains were not detected in the amylopectins by HPSEC measurement. The side‐chains measured by HPAEC were classified into four groups according to their degree of polymerization (DP), and the proportion of each group were in the following ranges: DP 6‐12, 26.5‐27.5%; DP 13‐24, 43.6‐44.1%; DP 25‐36, 13.6‐14.2%, and DP 37‐60, 11.0‐11.7%. The alleles on the Wx‐D1 locus, i.e., Wx‐D1a, Wx‐D1d, Wx‐D1f, and Wx‐D1g, responsible for granule‐bound starch synthase (GBSS I) biosynthesis had no influence on the properties of iodine‐amylopectin complex and the chain‐length distribution profiles of amylopectin. 相似文献
70.