Combinatorial Measurement of CDKN1A/p21 and KIF20A Expression for Discrimination of DNA Damage-Induced Clastogenicity

In vitro mammalian cytogenetic tests detect chromosomal aberrations and are used for testing the genotoxicity of compounds. This study aimed to identify a supportive genomic biomarker could minimize the risk of misjudgments and aid appropriate decision making in genotoxicity testing. Human lymphoblastoid TK6 cells were treated with each of six DNA damage-inducing genotoxins (clastogens) or two genotoxins that do not cause DNA damage. Cells were exposed to each compound for 4 h, and gene expression was comprehensively examined using Affymetrix U133A microarrays. Toxicogenomic analysis revealed characteristic alterations in the expression of genes included in cyclin-dependent kinase inhibitor 1A (CDKN1A/p21)-centered network. The majority of genes included in this network were upregulated on treatment with DNA damage-inducing clastogens. The network, however, also included kinesin family member 20A (KIF20A) downregulated by treatment with all the DNA damage-inducing clastogens. Downregulation of KIF20A expression was successfully confirmed using additional DNA damage-inducing clastogens. Our analysis also demonstrated that nucleic acid constituents falsely downregulated the expression of KIF20A, possibly via p16 activation, independently of the CDKN1A signaling pathway. Our results indicate the potential of KIF20A as a supportive biomarker for clastogenicity judgment and possible mechanisms involved in KIF20A downregulation in DNA damage and non-DNA damage signaling networks.     

Lessons from Earthquakes in Recent Times and their Effects on the Development of Geotechnology

This paper first reviews the earthquakes that occurred since 1995 and then outlines the significant lessons learnt from disasters. The discussion then goes into the induced development of engineering practices that were triggered by those disasters. It is clear that major development occurred in design philosophies, soil improvement, and facilities for research. The last section of this paper addresses the compound effects of earthquakes and rainfall that may make the extent of the disasters worse.     

A voltage-source PWM rectifier-inverter with feedforward control of instantaneous power

In general, diode rectifiers with electrolytic capacitors on the dc side have been used as dc power supplies for voltage-source inverters. Rectifiers of this type, however, cause many problems such as poor power factor and harmonics. Recently, voltage-source PWM rectifier-inverters have been studied to provide the following advantages: (1) harmonic-free on both ac sides; (2) unity power factor on the input ac side; (3) power flow of either direction or power regeneration; (4) reduction of the dc capacitor. However, it is difficult for a conventional voltage-source PWM rectifier-inverter to regulate the capacitor voltage on transient states because it has only a voltage feedback loop. This paper describes a voltage-source PWM rectifier-inverter with feedforward control of instantaneous power. Based on the pq theory, the instantaneous power which is calculated in the control circuit of the inverter is fed forward to the control circuit of the rectifier. The feedforward control of instantaneous power contributes greatly to sufficiently suppress voltage fluctuation of the dc capacitor on transient states. Transient characteristics are discussed, and some interesting experimental results of a laboratory model are shown.     

Systemic effects and withdrawal syndrome in chronic users of corticosteroids]

Three newly found vibrational levels at energies Te(v) = 45127.88(3), 51900.40(4), and 52604.95(3) cm-1 allow the first application of resonance-enhanced multiphoton ionization for detecting the SO radical in its low-energy singlet states, a1Delta and b1Sigma+. The analysis of the spectra is based on the observation of 10 rotationally resolved bands starting from a1Delta v = 0, 6 ellipsis 11 and b1Sigma+ v = 8, covering a typical range of J = 5 ellipsis 40 or 50. Population of these SO singlet states has been achieved by two-photon dissociation of SO2 in a molecular beam environment, applying wavelengths in the 248 to 291 nm range. This paper presents an analysis of the rotational, vibrational, and electronic properties of the new levels. Reviewing earlier works on the singlet states of SO, a revised term energy scheme is provided which connects the singlet and triplet systems of the molecule. Copyright 1998 Academic Press.