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排序方式: 共有596条查询结果,搜索用时 421 毫秒
91.
92.
Ogi H Fukunishi Y Omori T Hatanaka K Hirao M Nishiyama M 《Analytical chemistry》2008,80(14):5494-5500
In this paper, we developed a 55-MHz wireless-electrodeless quartz crystal microbalance (QCM) and systematically studied the effects of flow rate on the sensitivity to the detection of proteins and on the affinity between biomolecules evaluated by the flow injection system. Brownian motion of proteins in liquid suggests a low probability of meeting, and the convection effect plays an important role in the sensitivity and the affinity in the flow cell injection system. The wireless quartz crystal was isolated in the QCM cell, and flow rates between 50 and 1000 microL/min were used for monitoring binding reactions between human immunoglobulin G and Staphylococcus aureus protein A. The sensitivity was significantly increased as the flow rate increased, while the affinity value remained unchanged. However, the affinity value was affected by the reaction time for a large-concentration analyte, indicating the need of a high-sensitivity biosensor system for accurate evaluation of affinity. The electrode effect on the QCM sensitivity was also theoretically investigated, showing that the electrode significantly deteriorates the QCM sensitivity and makes the Sauerbrey equation invalid. 相似文献
93.
Photo-induced molecular alignment behavior of azo dye derivative (sodium 4,4′-bis (4-hydroxy-3-carboxy-phenylazo) benzidine-2,2′-disulphonate: SD1) was investigated using SD1 film prepared by spin-coating method. As-prepared SD1 film was composed of an amorphous layer with smooth surface. Upon linearly polarized UV light irradiation, the film surface was roughened slightly and X-ray diffraction measurement and transmittance electron microscope observation indicated that SD1 molecules crystallized with orientation. Molecular plane of SD1 aligned parallel to the substrate surface along the normal to the polarization direction of irradiated UV light through the trans-cis and cis-trans isomerizations of the azobenzene chromophore. 相似文献
94.
Jin-Long LiYong-Qiang Cheng Pan WangWen-Ting Zhao Li-Jun Yin Masayoshi Saito 《Food Hydrocolloids》2012,26(2):448-455
Extensive research has indicated that the electrostatic attraction between polysaccharides and proteins on the oil-water interface can improve the stability of emulsions. However, this electrostatic effect will be weakened or even eliminated as the solution pH or ionic strength of emulsions change, resulting in the shedding of the polysaccharide layer. We prepared primary oil-in-water emulsions at pH 7.0 using whey protein isolate (WPI) as an emulsifier and then beet pectin was added to form secondary emulsions. After the pH of emulsions was adjusted to 4.0 to promote electrostatic attraction between the beet pectin molecules and the protein-coated droplets, horseradish peroxidase was added to generate a cross-linked beet pectin coating. Results show that stable emulsions coated with WPI and cross-linked beet pectin interfaces could be formed. The sensitivity of the emulsions to the environmental stresses of pH changes, ions addition, thermal processing and freezing was also characterized in this work. Our results support the view that cross-linked beet pectin improves the stability of emulsions and is superior to simple deposition on the surface of lipid droplets. The interfacial engineering technology used in this study could be used to create food emulsions with improved stability to environmental stresses. 相似文献
95.
96.
We propose a thin-film bending technique based on the ion-induced bending (IIB) phenomenon, which enables the fabrication of three-dimensional structural devices and arrays, such as micro-electro mechanical system (MEMS) devices. We investigated the IIB phenomenon with various film materials and various ion species. It was found that the distribution of vacancy occurs under ion-irradiation was the important parameter affecting the degree of bending, irrespective of film material and ion-species. Therefore, it was found that the bending angle could be controlled by the distribution of vacancy. Using this technique, a micron-sized region of a standing thin-film array could be produced using conventional ion-implantation equipment used in semiconductor manufacturing. 相似文献
97.
Ueda S Kinoshita M Tanaka F Tsuboi M Shimizu S Oohata N Hino M Yamada M Isogai Y Hashimoto S 《Journal of Bioscience and Bioengineering》2011,112(4):409-414
Micafungin (FK463) is a widely used treatment for life-threatening, deep-seated fungal infections. It is an echinocandin-like lipopeptide derived from the chemical modification of deacylated FR901379, a type of lipopeptide antibiotic produced by Coleophoma empetri F-11899. The palmitoyl moiety of FR901379 is deacylated by FR901379 acylase produced by Streptomyces sp. no. 6907. In this study, our goal was to generate an improved strain of Streptomyces sp. no. 6907 capable of hyperproducing the FR901379-acylase enzyme. To accomplish this goal, modified strains of Streptomyces sp. no. 6907 were generated using UV-irradiation mutagenesis, and strain selection was performed using an agar-plate screening method to efficiently select an acylase-hyperproducing strain. Three marker indices were shown to correlate with elevated acylase production: decreased candidacidal activity of FR901379, decreased proteolytic activity on skim milk, and phenotypic characteristics. Cloning and subsequent sequencing of the acylase gene from the hyperproducing mutant revealed no mutations in either the acylase structural gene or the 5'-flanking region required for gene expression. The growth medium was also modified to maximize acylase production. We successfully increased acylase activity approximately 65-fold, compared with the original growth conditions (wild strain cultured in the original unmodified medium). To minimize formation of excess foam during the fermentation process, we optimized the parameters of agitation speed, as calculated from the discharge flow rate. Using our improved strain and the optimized medium and growth conditions, we have developed an improved and highly reproducible method for stable large-scale production of FR901379-acylase. 相似文献
98.
In this paper, a tele-monitoring system is proposed, using an inertial measurement unit (IMU) and a shoe-type ground reaction force (GRF) sensor called a Smart Shoe to measure a patient’s walking data, and transmitting the measured data via the Internet. In our previous work, a mobile gait-monitoring system was developed, which provided visual feedback based on GRFs measured by a Smart Shoe (used as a mobile platform). However, the limited information provided by the Smart Shoe alone may not be adequate for a tele-monitoring system using the Internet. In the present tele-monitoring system for gait rehabilitation, a Smart Shoe is combined with an IMU for detailed monitoring of walking motions. By analyzing the signals from the IMU and the Smart Shoe, foot trajectories, walking distance, length of stride, etc., can be estimated. A user-friendly graphic interface displays the measured or estimated data on separate computers at the patient’s location and the physical therapist’s office. Thus, using the proposed system, it is possible to monitor a patient’s walking motion via the Internet, without restrictions on time or place. 相似文献
99.
Yuri Avetisyan Caihong Zhang Masayoshi Tonouchi 《Journal of Infrared, Millimeter and Terahertz Waves》2012,33(10):989-998
A new scheme of optical rectification of femtosecond laser pulses in a periodically poled lithium niobate crystal, which generates high energy and linewidth tunable multicycle terahertz (THz) pulses, is analyzed. The developed simple theoretical model allows investigating the generated THz spectrum and its dependence on spot size of the pump beam. It is shown that the transformation of THz radiation from narrowband to broadband is possible by simply reducing the pump beam size. The temporal waveform and energy of the multicycle THz pulses were calculated as well. It is shown that THz energy is inversely proportional to the pump beam size r y , whereas energy spectral density is independent of r y . The efficiency of optical-to-THz energy conversion for pump pulse energy of 1?mJ is estimated to be 0.8?×?10?4. The possibility of tuning the generation frequency by changing the direction of the pump beam propagation is analyzed. 相似文献
100.
Randles AB Esashi M Tanaka S 《IEEE transactions on ultrasonics, ferroelectrics, and frequency control》2010,57(11):2372-2380
This paper presents the etch rate of lithium niobate (LiNbO(3)) as a function of crystal orientation. Etching is a fundamental technology needed for the fabrication of new sensors, actuators, and other new devices. In this study, LiNbO(3) spheres 30 mm in diameter were etched in hydrofluoric acid and a mixture of hydrofluoric and nitric acids at different temperatures and different times. The measured data of the etched sphere shape were processed and plotted, giving etch rate diagrams over the entire spheres. Based on the etch rate data obtained, the Wulff-Jaccodine method was used to predict the etched shape of 128° Y-cut and 155° Y-cut LiNbO(3). The predicted etching profiles were compared with those obtained by experiments. A least-square polynomial fit for the data was also developed and was found to be useful in removing some of the variation in the measurements. 相似文献