CBF beta-SMMHC, expressed in M4Eo AML, reduced CBF DNA-binding and inhibited the G1 to S cell cycle transition at the restriction point in myeloid and lymphoid cells

CBF beta-SMMHC is expressed from the inv(16) chromosome in M4Eo AML. Mice lacking CBF subunits or expressing the CBF beta-SMMHC or AML1-ETO oncoproteins failed to develop definitive hematopoiesis. To investigate these effects on hematopoiesis, we expressed CBF beta-SMMHC from the metallothionein promoter, in both 32D cl3 myeloid cells and Ba/F3 B-lymphoid cells. Addition of zinc increased CBF beta-SMMHC levels more than tenfold, with higher levels evident in Ba/F3 lines. Levels obtained in 32D cl3 cells were similar to those of endogenous CBF beta. Indirect immunofluorescence revealed zinc-inducible speckled, nuclear staining in Ba/F3 cells and diffuse nuclear staining in 32D cl3 cells. CBF beta-SMMHC reduced endogenous CBF DNA-binding fivefold in both cell types, increased cell generation time 1.9-fold, on average, in 32D cl3 cells and 1.5-fold in Ba/ F3 cells and decreased tritiated thymidine incorporation into DNA correspondingly. CBF beta-SMMHC increased the proportion of cells in G1 1.7-fold, on average, in 32D cl3 and Ba/F3 cells, and decreased the proportion of cells in S phase by a similar degree. CBF beta-SMMHC induced a marked increase in hypophosphorylated Rb, but did not alter IL-3 Receptor alpha or beta subunit levels. Neither apoptosis nor 32D differentiation was induced by zinc in IL-3 in these lines. Induction of CBF beta-SMMHC in 32D cl3 cells did not inhibit their differentiation to neutrophils or their expression of myeloperoxidase mRNA in G-CSF, and did not produce an eosinophilic phenotype. Additional, proliferative genetic changes in M4eo AMLs might potentiate inhibition of differentiation by CBF beta-SMMHC by allowing its increased expression.     

导电环接触电阻的改进测量方案

介绍了导电环接触电阻测量方法的现状,提出了在测量过程中防止导线缠绕的两种改进方案,通过分析验证,说明改进后的测量方案能够很好的完成导电环接触电阻的测量.     

采取具体措施 发挥教研室的职能作用

本文就如何发挥高校教研室的职能作用记述了本教研室所采取的具体方法和措施。     

Recovery of second-order optical nonlinearity in annealedproton-exchanged LiNbOx

The second-order nonlinear coefficients measured in proton-exchanged LiNbO₃ as a function of annealing time are discussed. Measurements of reflected second-harmonic power indicate that the second-order nonlinear coefficient d₃₃ is reduced to 60% of the bulk value as a result of proton exchange in pure benzoic acid. It is also shown that annealing restores the d-coefficients to almost the original value of the virgin crystal. For example, recovery to ~90% of the bulk value was obtained for a sample with a 0.3-μm-bulk proton-exchanged layer, annealed for 10 h at 350°C     

DG抗高温隔离液在古深1井的应用

古深1井是大庆油田在古龙地区的一口风险探井,设计井深5 500 m,实际完钻井深为4 739 m。钻进过程中,多次出现塌、漏、卡等事故,造成井径不规则。为平衡压力,压稳地层,更好地隔离钻井液和水泥浆,提高顶替效率,设计采用DG抗高温隔离液。DG抗高温隔离液抗高温可达150℃,密度调节范围宽,抗高温悬浮稳定性好,失水量少,与水泥浆和钻井液相容性好。古深1井采用双级注水泥工艺,固井施工过程十分顺利,隔离液密度均匀,泵送容易,无发泡现象。从声幅测井情况来看,水泥面上部与隔离液交界处界面清楚,水泥环胶结良好,水泥返高达到了设计要求,混浆过渡段短,固井质量合格。     

皖河闸安全复核分析评价

本文针对皖河闸工程在运行中暴露出来的问题,以定性和定量相结合的方法综合分析皖河闸工程安全状况,并确定该闸安全类别,为工程改建提供依据。     

The forward muon detector of the DELPHI experiment at LEP

The layout and construction of the muon drift chambers equipping the end caps of the DELPHI detector are described. The performance of this forward muon detection system, determined from cosmic ray tests and data collected during the first year of LEP operation, is presented.     

基于WebGIS的粗泥沙集中来源区电子地图系统研究

基于WebGIS的粗泥沙集中来源区电子地图系统建设，以粗泥沙集中来源区作为研究区域，以黄河中游粗泥沙集中来源区水土保持工作流程（包括规划设计、计划管理、综合治理、预防监督和淤地坝管理等）为主线，依托黄河流域水土保持生态与环境监测系统网络、黄河流域水土保持监测系统数据库，建立覆盖黄河流域、黄土高原区、多沙粗沙区、粗泥沙集中来源区，面向不同水土保持管理层的水土保持监测与管理信息系统。     

在线安全预警和决策支持系统的软件构架与实现

安全预警和决策支持（EWSC）系统作为未来控制中心自动化系统的重要功能，一方面需要对能量管理系统（EMS）成熟功能进行封装，另一方面需要适应自动化系统功能演变的需要。提出了一种基于多代理系统（MAS）的软件开发范式，用于开发EWSC系统的软件构架。该系统具有一个松散、开放的可插入式结构。其中核心框架是负责系统通信和任务调度的协调层，是相对固定的;其余部分为独立模块，由一系列相对独立的决策分析和人机表达智能体组成，可以独立开发，方便地嵌入系统。在该框架下系统各个模块层次分明，具有很强的解耦性，并且可以实现把历史遗留系统集成到新的环境中。基于文中所提出的软件构架的在线EWSC系统已在某省级电网投运。     

无线局域网采用的补偿码键控调制方式

采用补偿码健控（CCK）可以使IEEE802．11b无线局域网系统实现多速率通信，这是补偿码第一次用于调制。介绍了无线局域网的直接序列扩频（DSSS）的各种模式，着重介绍了无线局域网所采用的CCK调制方式，并对CCK调制的性能进行了理论上的分析。