Flavonoids from Machilus japonica Stems and Their Inhibitory Effects on LDL Oxidation

Stems of Machilus japonica were extracted with 80% aqueous methanol (MeOH) and the concentrated extract was successively extracted with ethyl acetate (EtOAc), normal butanol (n-BuOH), and water. Six flavonoids were isolated from the EtOAc fraction: (+)-taxifolin, afzelin, (−)-epicatechin, 5,3''-di-O-methyl-(−)-epicatechin, 5,7,3''-tri-O-methyl-(−)-epicatechin, and 5,7-di-O-methyl-3'',4''-methylenedioxyflavan-3-ol. The chemical structures were identified using spectroscopic data including NMR, mass spectrometry and infrared spectroscopy. This is the first report of isolation of these six compounds from M. japonica. The compounds were evaluated for their diphenyl picryl hydrazinyl scavenging activity and inhibitory effects on low-density lipoprotein oxidation. Compounds 1 and 3–6 exhibited DPPH antioxidant activity equivalent with that of ascorbic acid, with half maximal inhibitory concentration (IC₅₀) values of 0.16, 0.21, 0.17, 0.15 and 0.07 mM, respectively. The activity of compound 1 was similar to the positive control butylated hydroxytoluene, which had an IC₅₀ value of 1.9 µM, while compounds 3 and 5 showed little activity. Compounds 1, 3, and 5 exhibited LDL antioxidant activity with IC₅₀ values of 2.8, 7.1, and 4.6 µM, respectively.     

Measurement of through-the-thickness variations of mechanical properties in SA508 Gr.3 pressure vessel steels using ball indentation test technique

The through-the-thickness variations of mechanical properties in SA508 Gr.3 pressure vessel steels were measured using the automated ball indentation (ABI) test technique. Key mechanical properties, such as the yield strength, ultimate strength, flow curve and hardness, were evaluated from indentation load-depth curves. The mechanical properties measured were location-dependent and the steepest gradients in the distributions of the mechanical properties appeared in the near-surface regions. The maximum through-the-thickness variations of the mechanical properties were in the range of 5–20% and they depended on the manufacturing process as well as the original wall thickness. It was concluded that the through-the-thickness variations in the mechanical properties were mainly caused by the location-dependent cooling rate during water quenching in the quality heat treatment which consisted of water quenching and tempering.     

Cooperative Hybrid‐ARQ Protocols: Unified Frameworks for Protocol Analysis

Cooperative hybrid‐automatic repeat request (HARQ) protocols, which can exploit the spatial and temporal diversities, have been widely studied. The efficiency of cooperative HARQ protocols is higher than that of cooperative protocols because retransmissions are only performed when necessary. We classify cooperative HARQ protocols as three decode‐and‐forward‐based HARQ (DF‐HARQ) protocols and two amplified‐and‐forward‐based HARQ (AF‐HARQ) protocols. To compare these protocols and obtain the optimum parameters, two unified frameworks are developed for protocol analysis. Using the frameworks, we can evaluate and compare the maximum throughput and outage probabilities according to the SNR, the relay location, and the delay constraint. From the analysis we can see that the maximum achievable throughput of the DF‐HARQ protocols can be much greater than that of the AF‐HARQ protocols due to the incremental redundancy transmission at the relay.