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11.
Hartmut Rehbein Horst Karl 《Zeitschrift für Lebensmitteluntersuchung und -Forschung A》1985,180(5):373-378
Summary The extraction of fish muscle protein using SDS containing solubilization buffers was studied varying the time and the temperature of solubilization, as well as pH and SDS concentration of the buffer. At pH < 6 the myofbrillar proteins were incompletely solubilized; temperatures of 80-100 °C resulted in protein degradation observable in the SDS-PAGE.Samples of fish muscle containing high amounts of formaldehyde (50 mmoles FA/kg wet weight) could only be solubilised at 100 °C; on the other hand it was possible to solubilize cooked and/or canned products under mild conditions (2% SDS, 1% 2-ME, pH 8.9, shaking for 2 h at 60 °C).
Abbreviations FA formaldehyde - IEF isoelectric focusing - 2-ME 2-mercaptoethanol - PA polyacrylamide - PAGE polyacrylamide gel electrophoresis - pI isoelectric point - SDS sodium dodecyl sulfate - TMAO trimethylamine oxide - Tris Tris (hydroxymethyl)methylamine 相似文献
Solubilisierung von Fischmuskelproteinen durch Natriumdodecylsulfathaltige Puffer
Zusammenfassung Der Einfluß von Solubilisierungszeit und -temperatur, des pH-Wertes und der SDS-Konzentration des Solubilisierungspuffers auf die Extraktion von Fischmuskelproteinen wurde überprüft. Bei pH-Werten < 6 wurden die myofbrillären Proteine nur unvollständig gelöst; Solubilisierungstemperaturen von 80 bis 100 °C führten zu einem in der SDS-PAGE sichtbaren Proteinabbau. Fischmuskelproben mit hohem Formaldehydgehalt (50 mmole FA/kg Feuchtgewicht) ließen sich nur bei 100 °C vollständig solubilisieren; demgegenüber gelang die weitgehende Solubilisierung gegarter und/oder sterilisierter Produkte unter milden Bedingungen (2% SDS, 1% 2-ME, pH 8,9; 2stündiges Schütteln bei 60 °C).
Abbreviations FA formaldehyde - IEF isoelectric focusing - 2-ME 2-mercaptoethanol - PA polyacrylamide - PAGE polyacrylamide gel electrophoresis - pI isoelectric point - SDS sodium dodecyl sulfate - TMAO trimethylamine oxide - Tris Tris (hydroxymethyl)methylamine 相似文献
12.
13.
Summary Methacrylic acid was photopolymerised using CdS and composite, CdS/HgS and CdS/TiO2, colloidal semiconductor particles as initiators. The previously proposed photoinitiation mechanism involving the photogenerated positive hole in the valence band of the CdS colloid was confirmed by the electron scavenging action of TiO2. The effect of pH on the course of the polymerisation was investigated and is discussed. 相似文献
14.
van der Horst M.J. Linnenbank A.C. van Staveren A. 《Electromagnetic Compatibility, IEEE Transactions on》2005,47(1):34-44
The electromagnetic-interference effects of out-of-band signals on negative-feedback amplifiers are investigated. It is assumed that the interfering signals picked up at the input of the amplifier are dominant. Due to the nonlinear behavior of the active devices in the amplifier, unwanted dc shifts and amplitude-modulation (AM) detection may occur. Describing these effects is usually done by using the Volterra series. Although providing good results when used for analysis, methods to use the Volterra series for the design process are not yet mature enough. A simple procedure for calculating AM detection is presented. An equivalent source at the input of the amplifier is introduced that accounts for the nonlinear effects. Because the characteristics of this source are computed as a function of design parameters, this procedure facilitates a synthesis approach for designing amplifiers with a lower susceptibility to AM detection. It is shown that the root locus of the amplifier transfer function has a large influence on the amount of AM detection and, therefore, on the electromagnetic compatibility. Measurements made on a single-stage negative-feedback amplifier support the presented procedure. 相似文献
15.
The classical two-film model for mass transfer between two fluid phases can only be used to describe the steady state mass transfer mechanism. Both the penetration and the film-penetration model are also valid solely when only one diffusion boundary layer exists at the interface between the phases. With a view to surmounting the limitations of the above models a new model has been developed for the mass transfer between two fluid phases with two diffusion boundary layers at the interface. The new model is based on the two-film concept and the Danckwerts distribution function, and is also able to simulate the unsteady state mass transfer mechanism between two fluid phases with two different boundary layers. 相似文献
16.
17.
Robert Leeb Felix Lee Claudia Keinrath Reinhold Scherer Horst Bischof Gert Pfurtscheller 《IEEE transactions on neural systems and rehabilitation engineering》2007,15(4):473-482
The step away from a synchronized or cue-based brain-computer interface (BCI) and from laboratory conditions towards real world applications is very important and crucial in BCI research. This work shows that ten naive subjects can be trained in a synchronous paradigm within three sessions to navigate freely through a virtual apartment, whereby at every junction the subjects could decide by their own, how they wanted to explore the virtual environment (VE). This virtual apartment was designed similar to a real world application, with a goal-oriented task, a high mental workload, and a variable decision period for the subject. All subjects were able to perform long and stable motor imagery over a minimum time of 2 s. Using only three electroencephalogram (EEG) channels to analyze these imaginations, we were able to convert them into navigation commands. Additionally, it could be demonstrated that motivation is a very crucial factor in BCI research; motivated subjects perform much better than unmotivated ones. 相似文献
18.
Formulating the minimum concave cost capacitated network flow problem as an integer concave minimization problem, we establish
finite branch and bound algorithms, in which the branching operation is the so–called integral rectangular partition and the
bounding procedure is performed by the classical minimum linear cost flow problem on subnetworks. For the special case that
the flow cost function is concave on a fixed number of arcs and linear on the others, an upper bound of the running time is
given.
Received: 19 July 1996 / Accepted: 8 July 1997 相似文献
19.
Reinhard Fritsch 《真空研究与实践》1990,2(2):104-112
In Part 1 (Henning, Materials used in Vacuum Technology), the application possibilities of different materials in vacuum technology were described. Part 2 describes the most important non-detachable connections of these materials to produce components that can be used in vacuum technology. The following processes are suitable for this purpose:
- ○ Welding
- ○ Soldering
- ○ Glueing
- ○ Fusion
20.
Catalytically powerful, non-porous membranes were manufactured from two highly gas permeable poly(amide imides) consisting of structures with moieties of 3,3'-dimethylnaphthidine and hexafluoroisopropylidene (6F) or hexafluoroisopropylidene 2,2-bis(phthalic acid anhydride) (6FDA) and 6F. The catalysts are pure precious metals or precious metal alloys dispersed on a nanoscale uniformly throughout the membrane. The membranes are characterized by electron microscopy, gas permeability, hydrogen uptake and, as a model reaction, the decomposition of nitrous oxide by hydrogen to nitrogen and water catalyzed by Pd/Ag. The permeance to hydrogen and nitrous oxide is round 2–10-6 cm3 (STP) /cm2·s·cmHg for membranes of 40–50μm in thickness. 相似文献