Bioorthogonal Chemical Reporters for Monitoring Unsaturated Fatty‐Acylated Proteins

Dietary unsaturated fatty acids, such as oleic acid, have been shown to be covalently incorporated into a small subset of proteins, but the generality and diversity of this protein modification has not been studied. We synthesized unsaturated fatty‐acid chemical reporters and determined their protein targets in mammalian cells. The reporters can induce the formation of lipid droplets and be incorporated site‐specifically onto known fatty‐acylated proteins and label many proteins in mammalian cells. Quantitative proteomics analysis revealed that unsaturated fatty acids modify similar protein targets to saturated fatty acids, including several immunity‐associated proteins. This demonstrates that unsaturated fatty acids can directly modify many proteins to exert their unique and often beneficial physiological effects in vivo.     

Comparison of thin epitaxial film silicon photovoltaics fabricated on monocrystalline and polycrystalline seed layers on glass

We fabricate thin epitaxial crystal silicon solar cells on display glass and fused silica substrates overcoated with a silicon seed layer. To confirm the quality of hot‐wire chemical vapor deposition epitaxy, we grow a 2‐µm‐thick absorber on a (100) monocrystalline Si layer transfer seed on display glass and achieve 6.5% efficiency with an open circuit voltage (V_OC) of 586 mV without light‐trapping features. This device enables the evaluation of seed layers on display glass. Using polycrystalline seeds formed from amorphous silicon by laser‐induced mixed phase solidification (MPS) and electron beam crystallization, we demonstrate 2.9%, 476 mV (MPS) and 4.1%, 551 mV (electron beam crystallization) solar cells. Grain boundaries likely limit the solar cell grown on the MPS seed layer, and we establish an upper bound for the grain boundary recombination velocity (S_GB) of 1.6x10⁴ cm/s. Copyright © 2014 John Wiley & Sons, Ltd.     

Microstructure of the HMX‐Based PBX KS32 after Mechanical Loading

Non‐destructive X‐ray diffraction techniques were applied in order to monitor the influence of mechanical and shock‐loading on the microstructure of the plastic‐bonded high explosive KS32. The investigations uncovered damage to embedded coarse HMX crystals and to the binder system HTPB‐IPDI. Damage to the crystals occurred already during the kneading process in terms of deformation twinning. On higher loading between 400 MPa (static) and 480 MPa (dynamic) also crystal fracture was observed. The change in the binder structure was found after both static and dynamic loading, but not in the cured, differently kneaded samples. Moreover, the change in binder structure after dynamic loading was verified by dynamic mechanical analysis, and interpreted as a partial damage of the binder rubber shell around the explosive particles. The results are compared to literature data from imaging techniques.     

Effect of lubricating oil contamination on evaporation in refrigerants R12 and R22

An investigation has been made into the effect of oil concentration on evaporation heat transfer coefficients in refrigerant-oil mixtures flowing in a horizontal tube. A new correlation is presented for heat transfer coefficients in convective evaporation of refrigerant-oil mixtures that predicts the results of the present study within approximately ±20%. The paper reports measurements of evaporation heat transfer coefficients in refrigerants R12 and R22, both oil-free and with two concentrations of Shell Clavus 32 oil. A 1·8 m long ⅜ in O/D copper tube (8·05 mm I/D) was used, at evaporation temperatures of −5°C, 0°C and +5°C. Heat flux and mixture mass velocity were kept constant at 2500 W m⁻² and 155 kg m⁻² s⁻¹, respectively, and measured coefficients were in the range of 1400 to 3900 W m⁻² K⁻¹. The results showed that, for a complete evaporator, 2% oil may be expected to increase the heat transfer coefficient by 12%, but 10% oil returns the coefficient to oil-free values.     

Permeability characterization. Part 1: A proposed standard reference fabric for permeability

A three-dimensionally woven fabric is proposed as a standard reference material for permeability characterization. The 3-D woven fabric requires care in cutting and handling, although it is more robust than 2-D woven or braided fabrics. If prepared carefully, the permeability of the 3-D woven fabric can be measured reproducibly within 15% in either radial flow or saturated 1-D flow geometries. The material was characterized for permeability in radial, unsaturated and saturated 1-D, and through-thickness flow geometries. The transient results demonstrated the importance of structural heterogeneity on the unsaturated flow behavior, and agree qualitatively with a simplistic model of flow in heterogeneous unsaturated porous media. The effects of heterogeneity were manifested in the proposed SRM by an increasing trend in the “unsaturated permeability.” Experiments were also conducted with a random mat that displayed transient flows dominated by wicking. The effects of wicking on the macroscopic flow behavior were manifested by transients in the “unsaturated permeability” in which a decreasing trend was observed.     

Spatial planning for landscape: mapping the pitfalls

While welcoming the European Landscape Convention and acknowledging the successes of conservation organizations such as the National Trust in Britain, the Czech Village Restoration Scheme and the French eco‐musées, this paper identifies some of the pitfalls that must be avoided if the fine words of the Convention are to be translated into effective action. These include problems with designation, conflicts between different types of conservation, and the side effects of gentrification and increased tourism. There is a call for more research to break down barriers to understanding between countries, between disciplines, and between academics and practitioners.     

Changes in the acyl and alkenyl group composition of cardiac phospholipids in boars fed corn oil or rapeseed oil

Boars fed diets containing rapeseed oil for 8 weeks showed significantly higher levels of neutral lipids and similar levels of phospholipids, compared to those fed corn oil. Erucic and eicosenoic acids were found to be high in ethanolamine phosphoglycerides, and in particular alkenyl acyl-ethanolamine phosphoglyceride. Furthermore, both long chain monoenes were incorporated preferentially in position 2 of the choline and ethanolamine phosphoglycerides. The alkenyl group composition of the cardiac lipids of pigs was influenced by dietary fatty acids. When rapeseed oil was fed, small amounts of 20∶1 and 22∶1 alkenyl constituents were detected. Contribution No. 641 Animal Research Institute     

厌氧氨氧化技术用于高氨废水脱氮

在简要介绍厌氧氨氧化菌生理生化特性的基础上,总结了目前应用厌氧氨氧化的主要工艺及其工程实例,重点分析了厌氧氨氧化技术在处理高氨废水实际工程应用过程中需要解决的关键技术,为厌氧氨氧化技术在污水处理工程中的推广提供技术借鉴.     

Influence of temperature and the role of chromium on the kinetics of sulfidation of 310 stainless steel

The sulfidation of 310 stainless steel was studied over the temperature range from 910 to 1285° K. By adjusting the ratio of hydrogen to hydrogen sulfide, variations in sulfur potential were obtained. The effect of temperature on sulfidation was determined at three different sulfur potentials: 39 N·m^–2, 1.4×10^–2 N·m^–2, and 1.5×10^–4 N·m^–2. All sulfide scales contained one or two surface layers in addition to a subscale. The second outer layer (OL-II), furthest from the alloy, contained primarily Fe-Ni-S. The first outer layer0 (OL-I), nearest the subscale, contained Fe-Cr-S. The subscale consisted of sulfide inclusions in the metal matrix. Two different phases were observed in OL-II depending on the temperature and sulfur potential. Below 1065°K OL-II is composed of a mixture of monosulfides of iron and nickel (Fe Ni)_1–xS and pentlandite (Fe_4.5Ni_4.5S₈) with the pentlandite phase exsolved as lamellae upon cooling. At temperatures higher than 1065°K only the pentlandite phase was formed, which melted above 1145°K at sulfur potentials greater than 10^–2 N·m^–2, yielding metal-rich iron-nickel-sulfur. Above 1145°K, and at sulfur potentials less than 10^–2 N·m^–2, OL-II ceased to exist (this temperature is termed transition temperature). Below the transition temperature, where OL-II exists, OL-I could be represented by the general composition (Fe, Cr)_1–xS. This phase on cooling transformed into an array of structures differing in FeCr ratio. These substructures, however, were not observed in quenched samples. Above the transition temperature OL-I changed to a chromium-rich sulfide composition and was associated with a sudden decrease in reaction rate. Subscale formation was found to be due to the dissociation of OL-I at the scale-metal interface, and the extent of subscale growth was found to depend on the temperature and the sulfur potential, as well as the composition of OL-I. At a given temperature and sulfur potential the weight-gain data obeyed the parabolic rate law after an initial transient period. The parabolic rate constants obtained at the sulfur potential of 39 N·m^–2 did not show a break when the logarithm of the rate constant was plotted as a function of the inverse of absolute temperature. Sulfidation carried out at a sulfur potential below 2 × 10^–2 N·m^–2, however, did show a break at 1145°K. This break was found to be associated with the changes which had occurred in the FeCr ratio of OL-I. Below the transition temperature the activation energy was found to be approximately 125 kJ · mole^–1. Above the transition temperature the rate of sulfidation decreased with temperature but depended on the FeCr ratio in the ironchromium-sulfide layers of the OL-I. A reaction mechanism consistent with the experimental results has been proposed in which the diffusion of cations through OL-I is the rate-controlling step. Below the transition temperature the diffusion of Fe and Ni through OL-I contributes to the scale formation, whereas above the transition temperature the diffusion of Cr through OL-I controls the scale formation. Existing literature on the Fe-Ni-S system is compared with the present results.     

Effects of dissolved organic matter surrogates on the partitioning of 17beta-estradiol and p-nonylphenol between synthetic membrane vesicles and water

Partition coefficients of two estrogenic compounds, 17beta-estradiol (E2) and p-nonylphenol (NP), between synthetic membrane vesicles (K'lipw values) and water were determined using equilibrium dialysis to evaluate the potential biological uptake of these compounds with and without the presence of dissolved natural organic matter (DOM) surrogates, Suwannee River fulvic acid, dialyzed Aldrich humic acid, and polyphenolic tannic acid. Overall, K'lipw values for E2 and NP reduced by 20-30% with the increase of DOM concentration from 0 to approximately 4 mg of C/L, a typical DOM concentration in the aquatic environment. This trend for E2 and NP is similar to that obtained by other researchers for moderately hydrophobic compounds including polycyclic aromatic hydrocarbons with three or four rings. A three-phase compartment model with three independent interactions, the sorption of E2 or NP by DOM surrogate (Koc), the sorption of DOM surrogates by membrane vesicles (KlipDOM), and the partition of E2 or NP by membrane vesicles (Klipw), was proposed, and K'lipw values for E2 or NP in the presence of DOM were calculated. The model predicted the decrease in Klipw values with the increase of DOM concentration, and the predictions using the three linear interactions agreed satisfactorily with the experimental results at relatively lower concentration of DOM.