全文获取类型
收费全文 | 109篇 |
免费 | 6篇 |
专业分类
电工技术 | 1篇 |
化学工业 | 37篇 |
金属工艺 | 1篇 |
机械仪表 | 2篇 |
建筑科学 | 4篇 |
能源动力 | 10篇 |
轻工业 | 18篇 |
无线电 | 2篇 |
一般工业技术 | 18篇 |
冶金工业 | 7篇 |
自动化技术 | 15篇 |
出版年
2023年 | 2篇 |
2022年 | 1篇 |
2021年 | 1篇 |
2020年 | 3篇 |
2019年 | 1篇 |
2018年 | 1篇 |
2017年 | 1篇 |
2016年 | 2篇 |
2015年 | 2篇 |
2014年 | 3篇 |
2013年 | 7篇 |
2012年 | 5篇 |
2011年 | 3篇 |
2010年 | 2篇 |
2009年 | 3篇 |
2008年 | 8篇 |
2007年 | 4篇 |
2006年 | 7篇 |
2005年 | 2篇 |
2004年 | 4篇 |
2003年 | 2篇 |
2002年 | 2篇 |
2001年 | 4篇 |
2000年 | 1篇 |
1999年 | 3篇 |
1998年 | 3篇 |
1997年 | 4篇 |
1996年 | 8篇 |
1995年 | 1篇 |
1994年 | 2篇 |
1993年 | 2篇 |
1992年 | 2篇 |
1990年 | 6篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1987年 | 3篇 |
1985年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1971年 | 1篇 |
1966年 | 2篇 |
1956年 | 1篇 |
排序方式: 共有115条查询结果,搜索用时 0 毫秒
21.
Tjeenk Marjjke Leuveling; Bulsink Yvonne B. M.; Slotboom Arend J.; Verheij Hubertus M.; Haas Gerard H.de; Demleitner Gaby; Gotz Friedrich 《Protein engineering, design & selection : PEDS》1994,7(4):579-583
The Staphylococcus hyicus lipase is an acyl hydrolase with broadsubstrate specificity including neutral glycerides and phospholipids.To obtain further insight into the mechanism of action of thisenzyme, we tested several sulfonyl fluorides as active site-directedinhibitors. The enzyme is resistant to the well-known serineprotease/esterase inhibitor phenylmethanesulfonyl fluoride (PMSF),but is rapidly inactivated by hexadecylsulfonyl fluoride. Thekinetics of inactivation were studied in Triton X-100 micelles.Inactivation is fast and the rate of inactivation is constantover the pH range where this lipase is active. Metal ions likeCa2+ and Sr2+ do not appreciably influence the rate of inactivation,although the enzymatic activity is significantly increased,suggesting a structural role for these ions. The S.hyicus lipasecontains a consensus sequence G-H/Y-S-X-G. Substitution by site-directedmutagenesis of this serine (Ser369) by a cysteine resulted ina mutant with only 0.2% residual activity. The activity of thismutant could not be inhibited with water-soluble sulfhydrylreagents either in the presence or absence of Triton X-100 micelles.In the presence of Triton X-100 micelles, inactivation of themutant occurred with 4-nitrophenylhexadecyl disulfide (t1/2= 125 min) while the wild-type enzyme does not react at all.We conclude that Ser369 is the active site residue and thatin water this residue is inaccessible. Only after interfacialactivation Ser369 (or Cys369) becomes exposed and reacts withirreversible inhibitors. 相似文献
22.
de Boer AR Alcaide-Hidalgo JM Krabbe JG Kolkman J van Emde Boas CN Niessen WM Lingeman H Irth H 《Analytical chemistry》2005,77(24):7894-7900
The potential of high-temperature liquid chromatography (HTLC) was investigated in an on-line combination with a screening system for bioactive compounds against the enzyme cathepsin B. Samples were separated by HTLC and subsequently analyzed by an on-line continuous-flow enzymatic assay. Detection was performed by electrospray ionization mass spectrometry, revealing both the bioactivity and the molecular mass of the bioactive compounds. Compared to conventional reversed-phase liquid chromatography, the amount of methanol necessary for separation could be decreased to only 10%, which improved the compatibility of LC with a biochemical assay. Sufficient preheating of the mobile phase prior to the separation and postcolumn cooling to prevent deactivation of the enzyme, even at column temperatures as high as 208 degrees C, was achieved as indicated by the reliable peak shapes obtained. The sensitivity was comparable with previously described systems operating at ambient temperatures as similar IC50 values were obtained. Exposing the inhibitors to high temperatures did not lead to thermal decomposition. The separation of inhibitors and the subsequent biochemical assay was performed either isothermally at various temperatures or by applying various temperature gradients as well as at various flow rates. The results obtained clearly show the compatibility of HTLC with an enzymatic screening assay. 相似文献
23.
24.
Reinforcement learning on explicitly specified time scales 总被引:1,自引:0,他引:1
In recent years hierarchical concepts of temporal abstraction have been integrated in the reinforcement learning framework to improve scalability. However, existing approaches are limited to domains where a decomposition into subtasks is known a priori. In this article we propose the concept of explicitly selecting time scale related abstract actions if no subgoal related abstract actions are available. This concept is realised with multi-step actions on different time scales that are combined in one single action set. We exploit the special structure of the action set in the MSA-Q-learning algorithm. This approach is suited for learning optimal policies in unstructured domains where a decomposition into subtasks is not known in advance or does not exist at all. By learning different explicitly specified time scales simultaneously, we achieve a considerable improvement of learning speed, which we demonstrate on several benchmark problems. 相似文献
25.
Carmen García-Rosales Horst A. Schulze Aristides Naoumidis Hubertus Nickel 《Journal of the American Ceramic Society》1993,76(11):2869-2878
The quasi-ternary system Cr─Mn─Ti oxide was investigated at 1000°C under oxygen partial pressures ranging from 0.21 bar to 10-21 bar (1 bar = 105 Pa). X-ray diffraction analysis was used to identify phases and determine lattice parameters. The positions of phase boundaries as a function of oxygen partial pressure were measured using the emf method. The spinel MnCr2 O4 may be regarded as the most interesting compound in this system. Part of the chromium can be replaced by trivalent titanium at low oxygen partial pressures and by trivalent manganese at high pressures, and the formation of a limited solid solution with the spinel Mn2 TiO4 is possible in all cases. As a result, a coherent single-phase spinel region exists over the entire oxygen partial pressure range at 1000°C. 相似文献
26.
Charis Kalogirou Martin Spahn Markus Krebs Steven Joniau Evelyne Lerut Maximilian Burger Claus-Jürgen Scholz Susanne Kneitz Hubertus Riedmiller Burkhard Kneitz 《International journal of molecular sciences》2013,14(11):21414-21434
The treatment of high-risk prostate cancer (HRPCa) is a tremendous challenge for uro-oncologists. The identification of predictive moleculobiological markers allowing risk assessment of lymph node metastasis and systemic progression is essential in establishing effective treatment. In the current study, we investigate the prognostic potential of miR-205 in HRPCa study and validation cohorts, setting defined clinical endpoints for both. We demonstrate miR-205 to be significantly down-regulated in over 70% of the HRPCa samples analysed and that reconstitution of miR-205 causes inhibition of proliferation and invasiveness in prostate cancer (PCa) cell lines. Additionally, miR-205 is increasingly down-regulated in lymph node metastases compared to the primary tumour indicating that miR-205 plays a role in migration of PCa cells from the original location into extraprostatic tissue. Nevertheless, down-regulation of miR-205 in primary PCa was not correlated to the synchronous presence of metastasis and failed to predict the outcome for HRPCa patients. Moreover, we found a tendency for miR-205 up-regulation to correlate with an adverse outcome of PCa patients suggesting a pivotal role of miR-205 in tumourigenesis. Overall, we showed that miR-205 is involved in the development and metastasis of PCa, but failed to work as a useful clinical biomarker in HRPCa. These findings might have implications for the use of miR-205 as a prognostic or therapeutic target in HRPCa. 相似文献
27.
Thunnissen M.G.M. Marjolein; Franken Peet A.; de Haas Gerard H.; Drenth Jan; Kalk Kor H.; Verheij Hubertus M.; Dijkstra W. 《Protein engineering, design & selection : PEDS》1992,5(7):597-603
Tyr52 and Tyr73 are conserved amino acid residues throughoutall vertebrate phospholipases A2. They are part of an extendedhydrogen bonding system that links the N-terminal -NH+3 -groupto the catalytic residues His48 and Asp99. These tyrosines werereplaced by phenylalanines in a porcine pancreatic phospholipaseA2 mutant, in which residues 6266 had been deleted (6266PLA2).The mutations did not affect the catalytic properties of theenzyme, nor the folding kinetics. The stability against denaturatlonby guanidine hydrochloride was decreased, however. To analysehow the enzyme compensates for the loss of the tyrosine hydroxylgroup, the X-ray structures of the Y52F and AY73F mutants weredetermined. After crystallographic refinement the final crystallographicR-factors were 18.1% for the %Y52F mutant (data between 7 and2.3 Å resolution) and 19.1% for the Y73F mutant (databetween 7 and 2.4 Å resolution). No conformational changesoccurred in the mutants compared with the 6266PLA2, butan empty cavity formed at the site of the hydroxyl group ofthe former tyrosine. In both mutants the Asp99 side chain losesone of its hydrogen bonds and this might explain the observeddestabilization. 相似文献
28.
Hubertus Hohl Heinz-Dieter Böcker Rul Gunzenhäuser 《User Modeling and User-Adapted Interaction》1996,6(2-3):131-156
We have developed an adaptive hypertext system designed to individually support exploratory learning and programming activities in the domain of Common Lisp. Endowed with domain-specific knowledge represented in a hyperspace of topics, the system builds up a detailed model of the user's expertise which it utilizes to provide personalized assistance. Unlike other work emerging in the field of adaptive hypertext systems, our approach exploits domain and user modelling techniques to support individuals in different ways. The system not only generates individualized presentations of topic nodes, but also provides adaptive navigational assistance for link-based browsing. By identifying and suggesting useful hyperlinks according to the user's knowledge state and preferences, the system encourages and guides exploration. While browsing through the hyperspace of topics, the system analyses the user's navigational behaviour to infer the user's learning progress and to dynamically adapt presentations of topics and links accordingly. 相似文献
29.
Enantioselective palladium-catalyzed transformations 总被引:1,自引:0,他引:1
30.