全文获取类型
收费全文 | 165篇 |
免费 | 9篇 |
专业分类
电工技术 | 2篇 |
化学工业 | 38篇 |
金属工艺 | 2篇 |
机械仪表 | 2篇 |
建筑科学 | 9篇 |
矿业工程 | 1篇 |
能源动力 | 5篇 |
轻工业 | 6篇 |
水利工程 | 1篇 |
无线电 | 5篇 |
一般工业技术 | 34篇 |
冶金工业 | 22篇 |
自动化技术 | 47篇 |
出版年
2022年 | 3篇 |
2021年 | 7篇 |
2020年 | 3篇 |
2019年 | 4篇 |
2018年 | 4篇 |
2017年 | 2篇 |
2016年 | 3篇 |
2015年 | 6篇 |
2014年 | 10篇 |
2013年 | 13篇 |
2012年 | 8篇 |
2011年 | 19篇 |
2010年 | 8篇 |
2009年 | 4篇 |
2008年 | 15篇 |
2007年 | 7篇 |
2006年 | 6篇 |
2005年 | 7篇 |
2004年 | 3篇 |
2003年 | 3篇 |
2002年 | 3篇 |
2001年 | 1篇 |
2000年 | 4篇 |
1999年 | 4篇 |
1998年 | 5篇 |
1997年 | 1篇 |
1996年 | 6篇 |
1995年 | 3篇 |
1994年 | 2篇 |
1993年 | 3篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1979年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1973年 | 1篇 |
排序方式: 共有174条查询结果,搜索用时 15 毫秒
171.
Juillerat A Heinis C Sielaff I Barnikow J Jaccard H Kunz B Terskikh A Johnsson K 《Chembiochem : a European journal of chemical biology》2005,6(7):1263-1269
Fusion proteins of human O(6)-alkylguanine-DNA alkyltransferase (AGT) can be specifically labeled with a wide variety of synthetic probes in mammalian cells; this makes them an attractive tool for studying protein function. However, to avoid undesired labeling of endogenous wild-type AGT (wtAGT), the specific labeling of AGT fusion proteins has been restricted to AGT-deficient mammalian cell lines. We present here the synthesis of an inhibitor of wtAGT and the generation of AGT mutants that are resistant to this inhibitor. This enabled the inactivation of wtAGT and specific labeling of fusion proteins of the AGT mutant in vitro and in living cells. The ability to specifically label AGT fusion proteins in the presence of endogenous AGT, after brief incubation of the cells with a small-molecule inhibitor, should significantly broaden the scope of application of AGT fusion proteins for studying protein function in living cells. 相似文献
172.
Enhanced Two‐Photon Fluorescence Imaging and Therapy of Cancer Cells via Gold@Bridged Silsesquioxane Nanoparticles 下载免费PDF全文
173.
Glastre W Lacot E Jacquin O Hugon O de Chatellus HG 《Journal of the Optical Society of America. A, Optics, image science, and vision》2012,29(4):476-485
In this paper, we compare the sensitivity of two imaging configurations, both based on laser optical feedback imaging (LOFI). The first one is direct imaging, which uses conventional optical focalization on target, and the second one is made by a synthetic aperture (SA) laser, which uses numerical focalization. We show that SA configuration allows us to obtain good resolutions with high working distance and that the drawback of SA imagery is that it has a worse photometric balance in comparison to a conventional microscope. This drawback is partially compensated by the important sensitivity of LOFI. Another interest of SA relies on the capacity of getting three-dimensional information in a single x-y scan. 相似文献
174.
Natural organic matter (NOM) and pesticides removal using a combination of ion exchange resin and powdered activated carbon (PAC) 总被引:8,自引:0,他引:8
The combination of anion exchange resins (AERs) and powdered activated carbon (PAC) was studied to remove both natural organic matter (NOM) and pesticides. Experiments were conducted with high dissolved organic carbon (DOC) surface water (about 6.0mg DOC/L) spiked with both atrazine and isoproturon. AERs, like MIEX and IRA938, showed up to 75% removal of DOC after 30min contact time. The addition of PAC after treatment with these AERs only slightly decreased the residual DOC from 1.4 to 1.2mg/L. Experiments conducted with high (200microg/L) and low (1microg/L) initial pesticide concentrations showed that simultaneous and successive combinations of AER and PAC significantly improve the removal of both pesticides compared with PAC treatment on raw water. The improvement of short-term adsorption kinetics was explained by the adsorption of pesticides on AERs (about 5%) and the removal of high molecular weight (MW) NOM structures by AERs that reduce pore blockage phenomena. For 24h contact time with PAC (adsorption isotherms), the benefit of AER treatment was lower, which indicates that the refractory DOC to AER treatment still competes through direct site competition mechanism. MIEX resin had a distinct behavior since the simultaneous treatment with PAC showed no benefit on pesticide adsorption. The presence of fine residues of MIEX was shown to interfere with PAC adsorption. 相似文献