Simultaneous visualisation of biofouling, organic and inorganic particle fouling on separation membranes.

Fouling is a major problem in membrane processes of water treatment. It can be caused by the deposition of inorganic and organic particulate material, and of microbial cells which may subsequently form biofilms. In practice, usually more than one foulant participates in the formation of membrane deposits. Knowledge of the composition of fouling layers is important for the development of appropriate countermeasures. For this purpose, an experimental system was established for the generation and microscopic visualisation of mixed deposits, using fluorescently labelled model foulants: (i) drinking-water bacteria stained with nucleic acid-specific dyes (biofouling), (ii) synthetic clay mineral laponite stained with rhodamine 6G (inorganic particle fouling), and (iii) fluorescently labelled polystyrene microspheres (organic particle fouling). Polycarbonate and polyethersulfone membranes were challenged with these foulants by dead-end filtration. On the basis of different fluorescent labels, the single foulants in these mixed deposits could be visualised separately by confocal laser scanning microscopy which, in combination with image analysis, allowed the generation of three-dimensional views of the complete deposits. This method offers the possibility for the estimation of quantitative surface coverage by foulants and for the determination of the efficacy of cleaning measures with respect to the removal of different foulants.     

Ceramics from mining by-products and alumina production wastes

The possibility of obtaining chemically stable ceramics in plastic molding from pastes based on comprehensive use of mining by-products (plagioporphyry, quartz-biotite shale), alumina production wastes (red mud), and polymineral clay was demonstrated. The mechanism of enhancing paste sintering on addition of finely disperse grog-flux and increasing the alkali resistance of the material (to 95%) in molding of the crystalline hematite-anorthite-quartz system was examined. __________ Translated from Steklo i Keramika, No. 2, pp. 17–20, February, 2007.     

Coding techniques for the noisy magnetic recording channel: astate-of-the-art report

Coding techniques for improving the reliability of information storage on noisy magnetic recording channels are considered. It is assumed that the Lorentzian channel model applies and that the retrieved signal is perturbed with additive white Gaussian noise. The immunity against additive noise of state-of-the-art codes such as DC-free, runlength-limited, and trellis codes are assessed     

Electron diffraction study of enzyme-hydrolyzed cotton samples

An electron diffraction study on continuously and repeatedly enzyme-hydrolyzed cotton samples was carried out. The electron diffraction pattern on repeatedly enzyme-treated sample reveals a cellulose I pattern with the absence of the 101 plane. This confirms that enzymatic hydrolysis of cotton fibers proceeds preferentially along the 101 plane, being a clevage plane.     

Biochemical quality assessment of broiler production using a hydrogen bacteria biomass in the diet

The authors studied the biochemical composition of the meat of broilers, eggs, liver and muscles of laying hens of 3 generations on a 5, 10, 25, 50 and 100% (broilers), 10 and 20% (laying hens) replacement of the animal protein quota in the diet by protein obtained from the hydrogen bacteria Alcaligenes eutrophus L-1. No deterioration of the quality of the produce was found from the standpoint of the main biochemical parameters.     

Yeast exo-β-glucanases can be used as efficient and readily detectable reporter genes in Saccharomyces cerevisiae

Yeast exo-1,3-β-glucanases are secretable proteins whose function is basically trophic and may also be involved in cell wall glucan hydrolytic processes. Since fluorescein di(β-D -glucopyranoside) is a fluorogenic substrate detectable and quantifiable by flow cytometry, it was used for testing the ability of the EXG1 gene product of Saccharomyces cerevisiae and its homologous gene in Candida albicans to function as reporter genes. These open reading frames were coupled to different promoters in multicopy plasmids, and exoglucanase activity quantified at flow cytometry. Exoglucanases were found to be useful tools for the study of promoter regions in S. cerevisiae. This technique has the advantage over other reporter gene systems—such as β-galactosidase fusions—that it does not require permeabilization of yeast cells and therefore it allows the recovery of viable cells—by sorting—after flow cytometry analysis.