Potential Use of Quantum Dots in Flow Cytometry

QDs may offer significant advantages in environmental and bead-based applications where the target cells need to be discriminated above background fluorescence. We have examined the possible applications of QDs for flow cytometric measurements (FCM) by studying their excitation - emission spectra and their binding to paramagnetic beads. We labelled beads with either QDs or a commonly-used fluorochrome (FITC) and studied their fluorescence intensity by FCM. Flow cytometric comparisons indicated that the minimum fluorophore concentration required for detection of QDs above autofluorescent background was 100-fold less than for FITC.     

Cluster methods for assessing research performance: exploring Spanish computer science

The objective of this paper is to propose a cluster analysis methodology for measuring the performance of research activities in terms of productivity, visibility, quality, prestige and international collaboration. The proposed methodology is based on bibliometric techniques and permits a robust multi-dimensional cluster analysis at different levels. The main goal is to form different clusters, maximizing within-cluster homogeneity and between-cluster heterogeneity. The cluster analysis methodology has been applied to the Spanish public universities and their academic staff in the computer science area. Results show that Spanish public universities fall into four different clusters, whereas academic staff belong into six different clusters. Each cluster is interpreted as providing a characterization of research activity by universities and academic staff, identifying both their strengths and weaknesses. The resulting clusters could have potential implications on research policy, proposing collaborations and alliances among universities, supporting institutions in the processes of strategic planning, and verifying the effectiveness of research policies, among others.     

Functional characterization of pressurized liquid extracts of Spirulina platensis

Three different parameters (temperature, solvent, and extraction time) were studied regarding to pressure liquid extraction (PLE) of antioxidant and antimicrobial compounds from Spirulina platensis. Two different antioxidant methods, β-carotene bleaching method and DPPH^• (2,2-Diphenyl-1-picrylhydrazyl hydrate) free radical scavenging assay, were used to determine the optimal PLE conditions for antioxidants extraction. The selected conditions were as follows: extraction temperature equal to 115 °C, extraction time equal to 15 min and ethanol as extracting solvent. The main antioxidant compounds found in this extract were identified as zeaxanthin, a myxoxanthophyll-like compound and very polar phenolic compounds. Moreover, antimicrobial activity of different PLE fractions was tested against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 11775, Candida albicans ATCC 60193, and Aspergillus niger ATCC 16404. Data obtained showed the hexane and petroleum ether extracts were slightly more active than ethanolic extracts. As for water extracts, none of them were active against the microorganisms tested. Data indicated that both 115 and 170 °C were the best extraction temperatures conditions in order to optimize the extraction of antimicrobial compounds, whereas 9 min was the optimal extraction time. Besides, C. albicans was the most sensitive microorganism to all Spirulina PLE extracts.     

New trends in food processing

In this work some of the newest trends in food processing are reviewed. This revision intends to provide an updated overview (including works published until February 2001) on the newest food processes, including food manufacturing, preservation, and control. Modern processes for food and food ingredients manufacturing based on membrane technology, super-critical fluid technology, and some applications of biotechnology are presented, mainly applied to obtain functional foods, "all-natural" enriched foods, probiotics and prebiotics. Also included is a critical assessment concerning non-thermal preservation techniques used for food preservation, such as high hydrostatic pressure, pulsed electric fields, ultrasound, pulsed light, hurdle systems, etc. Finally, a group of new analytical techniques (i.e., molecular techniques such as Polymerase Chain Reaction (PCR), food image analysis, and biosensors) and their use for food and process control is reviewed.     

A cooperative coevolutionary approach dealing with the skull–face overlay uncertainty in forensic identification by craniofacial superimposition

Craniofacial superimposition is a forensic process where photographs or video shots of a missing person are compared with the skull that is found. By projecting both photographs on top of each other (or, even better, matching a scanned three-dimensional skull model against the face photo/video shot), the forensic anthropologist can try to establish whether that is the same person. The whole process is influenced by inherent uncertainty mainly because two objects of different nature (a skull and a face) are involved. In previous work, we categorized the different sources of uncertainty and introduced the use of imprecise landmarks to tackle most of them. In this paper, we propose a novel approach, a cooperative coevolutionary algorithm, to deal with the use of imprecise cephalometric landmarks in the skull–face overlay process, the main task in craniofacial superimposition. Following this approach we are able to look for both the best projection parameters and the best landmark locations at the same time. Coevolutionary skull–face overlay results are compared with our previous fuzzy-evolutionary automatic method. Six skull–face overlay problem instances corresponding to three real-world cases solved by the Physical Anthropology Lab at the University of Granada (Spain) are considered. Promising results have been achieved, dramatically reducing the run time while improving the accuracy and robustness.     

Galerkin meshfree methods applied to the nonlinear dynamics of flexible multibody systems

Meshfree Galerkin methods have been developed recently for the simulation of complex mechanical problems involving large strains of structures, crack propagation, or high velocity impact dynamics. At the present time, the application of these methods to multibody dynamics has not been made despite their great advantage in some situations over standard finite element techniques.     

Trp89 in the lid ofHumicola lanuginosa lipase is important for efficient hydrolysis of tributyrin

To determine whether Trp89 located in the lid of the lipase (EC 3.1.1.3) fromHumicola lanuginosa is important for the catalytic property of the enzyme, site-directed mutagenesis at Trp89 was carried out. The kinetic properties of wild type and mutated enzymes were studied with tributyrin as substrate. Lipase variants in which Trp89 was changed to Phe, Leu, Gly or Glu all showed less than 14% of the activity compared to that of the wild type lipase. The Trp89Glu mutant was the least active with only 1% of the activity seen with the wild type enzyme. All Trp mutants had the same binding affinity to the tributyrin substrate interface as did the wild type enzyme. Wild type lipase showed saturation kinetics against tributyrin when activities were measured with mixed emulsions containing different proportions of tributyrin and the nonionic alkyl polyoxyethylene ether surfactant, Triton DF-16. Wild type enzyme showed a V_max=6000±300 mmol·min⁻¹·g⁻¹ and an apparent K_m=16±2% (vol/vol) for tributyrin in Triton DF-16, while the mutants did not show saturation kinetics in an identical assay. The apparent K_m for tributyrin in Triton DF-16 was increased as the result of replacing Trp89 with other residues (Phe, Leu, Gly or Glu). The activities of all mutants were more sensitive to the presence of Triton DF-16 in the tributyrin substrate than was wild type lipase. The activity of the Trp89Glu mutant was decreased to 50% in the presence of 2 vol% Triton DF-16 compared to the activity seen with pure tributyrin as substrate. Wild type lipase and all mutants except Trp89Glu had the same affinity for the substrate interface formed by 15.6 vol% tributyrin in Triton DF-16. The Trp89Glu mutant showed a lower affinity than all the other lipase variants for the interface of 15.6 vol% tributyrin in Triton DF-16. The study showed that Trp89 located in the lid ofH. lanuginosa lipase is important for the efficient hydrolysis of tributyrin and that this residue plays a role in the catalytic steps after adsorption of the lipase to the substrate interface.     

从过程洞察灵活性——回首25年前的房屋项目

二战以后,丹麦常用的建筑方法是带有十字承重墙的多层建筑,但该设计体系并不能很好地适应其后的建筑改造。因此丹麦住房与建设署于1983年颁布了条例来鼓励3～5层的非营利性住房的技术革新,以适应未来新的用户需求,增加建筑的灵活性。2012年春天,丹麦科技大学DTU管理学院创新性地开展了一个评估工作,目的是研究在25年的建筑发展中原始竞赛作品中的创意是如何实现的。本文采用的数据取自始建于20世纪80年代的15个居民区里的1000～1200个公寓房。本文基于评估得到的临时结果包括在建筑规划和施工阶段把建筑的持续改变能力做到最出色的前6位方案。     

The role of Glu87 and Trp89 in the lid of Humicola lanuginosa lipase

The importance of Glu87 and Trp89 in the lid of Humicola lanuginosalipase for the hydrolytic activity at the water/lipid interfacewas investigated by site-directed mutagenesis. It was foundthat the effect on the hydrolytic activity upon the replacementof Trp89 with Phe, Leu, Gly or Glu was substrate dependent TheTrp89 mutants displayed an altered chain length specificitytowards triglycerides, with a higher relative activity towardstriacetin and trioctanoin compared with tributyrin. Trp89 wasshown to be lessimportant in the hydrolysis of vinyl esterscompared with ethylesters and triglycerides. An exclusive effecton the acylation reaction rate by the mutation of Trp89 wasconsistent with the data. It is suggested that Trp89 is importantin the process of binding the acyl chain of thesubstrate intothe activesite for optimal acylation reaction rate. The Trp89Phemutation resulted in an increased hydrolytic activity towards2-alkylalkanoic acid esters. This is suggested to be due toreduction of unfavourable van der Waals contacts between Trp89and the 2-substituent of the substrate. Thus, in contrast tonatural substrates, Trp89 has a negative impact on the catalyticefficiencywhen substrates with bulky acyl chains are used. Incontrast to the Trp89 mutations, the effect on the hydrolyticactivity of the Glu87Ala mutation was almost substrate independent,35–70% activity of wild-type lipase. Areduction of boththe acylation and deacylation reaction was consistent with thedata.     

Can organic farming help to reduce N-losses?

This study is in two parts. In the first part, nitrogen (N) losses per unit of milk and meat in Danish conventional and organic pig and dairy farming were compared on the basis of farm data. In the second part, organic and conventional dairy farming were compared in detail, using modelling. N-surpluses at different livestock densities, fodder intensities, and soil types were simulated. Finally, simulated N-surpluses were used in national scenarios for conversion to organic dairy farming in Denmark. In Part one, pig farming was found to have a higher N-efficiency than dairy farming. Organic pig production had a lower N-efficiency and a higher N-surplus per kg meat than conventional pig production. The possibilities to reduce N-loss by conversion to organic pig production therefore appear to be poor. Organic dairy farming had a higher N-efficiency and a lower N-surplus per kg milk than conventional dairy farming. Conversion from conventional to organic dairy farming may therefore reduce N-losses. In Part two, a positive correlation between livestock density and N-surplus ha^-1 was found for dairy farming. For all simulated livestock densities, fodder feeding intensities and soil types, organic systems showed a lower N-surplus per unit of milk produced than conventional systems. National scenarios for dairy farming showed that the present Danish milk production could be achieved with a 24% lower total N-surplus if converted from intensive conventional farming to extensive organic farming. At the same time, N-surplus ha^-1 and N-surplus (t milk)^-1 would be lowered by 50% and 25% respectively. Changing from intensive to extensive conventional dairy farming with a livestock density equal to that in the organic scenario resulted in a reduction in N-surplus ha^-1 of 15%. It was concluded that a reduction in total N-loss from agriculture is possible by converting from conventional to organic dairy farming but at the cost of either lower production on the present dairy farm area, or the current production on a substantially larger area.