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61.
Ilaria Cristofolini Alberto Molinari Giovanni Straffelini Palloma Vieira Muterlle 《Materials & Design》2011
Aim of this work is proposing a systematic design approach for wear resistant components produced by Powder Metallurgy (PM). Wear is a cause of failure for many industrial PM parts. Gears and cams, for example, are subjected to rolling and rolling–sliding wear, dry or lubricated depending on the applications. The design criteria are mostly empirical, thus resulting fully conservative, sometimes excessively conservative. A need for defining design criteria for wear resistant PM parts is thus revealed, to exploit at best the specific characteristics of both technology and materials. 相似文献
62.
Fortunati E Latterini L Rinaldi S Kenny JM Armentano I 《Journal of materials science. Materials in medicine》2011,22(12):2735-2744
New nanocomposite films based on a biodegradable poly (DL-Lactide-co-Glycolide) copolymer (PLGA) and different concentration
of silver nanoparticles (Ag) were developed by solvent casting. In vitro degradation studies of PLGA/Ag nanocomposites were
conducted under physiological conditions, over a 5 week period, and compared to the behaviour of the neat polymer. Furthermore
the silver ions (Ag+) release upon degradation was monitored to obtain information on the properties of the nanocomposites during the incubation.
The obtained results suggest that the PLGA film morphology can be modified introducing a small percentage of silver nanoparticles
that do not affect the degradation mechanism of PLGA polymer in the nanocomposite. However results clearly evinced the stabilizing
effect of the Ag nanoparticles in the PLGA polymer and the mineralization process induced by the combined effect of silver
and nanocomposite surface topography. The Ag+ release can be controlled by the polymer degradation processes, evidencing a prolonged antibacterial effect. 相似文献
63.
Luca Conti Gina Elena Giacomazzo Barbara Valtancoli Mauro Perfetti Alberto Privitera Claudia Giorgi Patrick Severin Sfragano Ilaria Palchetti Sara Pecchioli Paola Bruni Francesca Cencetti 《International journal of molecular sciences》2022,23(21)
Ovarian cancer recurrence is frequent and associated with chemoresistance, leading to extremely poor prognosis. Herein, we explored the potential anti-cancer effect of a series of highly charged Ru(II)-polypyridyl complexes as photosensitizers in photodynamic therapy (PDT), which were able to efficiently sensitize the formation of singlet oxygen upon irradiation (Ru12+ and Ru22+) and to produce reactive oxygen species (ROS) in their corresponding dinuclear metal complexes with the Fenton active Cu(II) ion/s ([CuRu1]4+ and [Cu2Ru2]6+). Their cytotoxic and anti-tumor effects were evaluated on human ovarian cancer A2780 cells both in the absence or presence of photoirradiation, respectively. All the compounds tested were well tolerated under dark conditions, whereas they switched to exert anti-tumor activity following photoirradiation. The specific effect was mediated by the onset of programed cell death, but only in the case of Ru12+ and Ru22+ was preceded by the loss of mitochondrial membrane potential soon after photoactivation and ROS production, thus supporting the occurrence of apoptosis via type II photochemical reactions. Thus, Ru(II)-polypyridyl-based photosensitizers represent challenging tools to be further investigated in the identification of new therapeutic approaches to overcome the innate chemoresistance to platinum derivatives of some ovarian epithelial cancers and to find innovative drugs for recurrent ovarian cancer. 相似文献
64.
Ilaria Andreana Manuela Malatesta Maria Assunta Lacavalla Federico Boschi Paola Milla Valeria Bincoletto Carlo Pellicciari Silvia Arpicco Barbara Stella 《International journal of molecular sciences》2023,24(1)
Muscular dystrophies are a group of rare genetic pathologies, encompassing a variety of clinical phenotypes and mechanisms of disease. Several compounds have been proposed to treat compromised muscles, but it is known that pharmacokinetics and pharmacodynamics problems could occur. To solve these issues, it has been suggested that nanocarriers could be used to allow controlled and targeted drug release. Therefore, the aim of this study was to prepare actively targeted poly(lactide-co-glycolide) (PLGA) nanoparticles (NPs) for the treatment of muscular pathologies. By taking advantage of the high affinity for carnitine of skeletal muscle cells due to the expression of Na+-coupled carnitine transporter (OCTN), NPs have been actively targeted via association to an amphiphilic derivative of L-carnitine. Furthermore, pentamidine, an old drug repurposed for its positive effects on myotonic dystrophy type I, was incorporated into NPs. We obtained monodispersed targeted NPs, with a mean diameter of about 100 nm and a negative zeta potential. To assess the targeting ability of the NPs, cell uptake studies were performed on C2C12 myoblasts and myotubes using confocal and transmission electron microscopy. The results showed an increased uptake of carnitine-functionalized NPs compared to nontargeted carriers in myotubes, which was probably due to the interaction with OCTN receptors occurring in large amounts in these differentiated muscle cells. 相似文献
65.
Barbara Peruzzi Enrica Urciuoli Michela Mariani Laura Chioma Luigi Tomao Ilaria Montano Mattia Algeri Rosa Luciano Danilo Fintini Melania Manco 《International journal of molecular sciences》2023,24(1)
Excess body weight has been considered beneficial to bone health because of its anabolic effect on bone formation; however, this results in a poor quality bone structure. In this context, we evaluated the involvement of circulating extracellular vesicles in the impairment of the bone phenotype associated with obesity. Circulating extracellular vesicles were collected from the plasma of participants with normal weight, as well as overweight and obese participants, quantified by flow cytometry analysis and used to treat mesenchymal stromal cells and osteoblasts to assess their effect on cell differentiation and activity. Children with obesity had the highest amount of circulating extracellular vesicles compared to controls. The treatment of mesenchymal stromal cells with extracellular vesicles from obese participants led to an adipogenic differentiation in comparison to vesicles from controls. Mature osteoblasts treated with extracellular vesicles from obese participants showed a reduction in differentiation markers in comparison to controls. Children with obesity who regularly performed physical exercise had a lower circulating extracellular vesicle amount in comparison to those with a sedentary lifestyle. This pilot study demonstrates how the high amount of circulating extracellular vesicles in children with obesity affects the bone phenotype and that physical activity can partially rescue this phenotype. 相似文献
66.
Ilaria De Simone Constance C. F. M. J. Baaten Martine Jandrot-Perrus Jonathan M. Gibbins Hugo ten Cate Johan W. M. Heemskerk Chris I. Jones Paola E. J. van der Meijden 《International journal of molecular sciences》2022,23(18)
Platelet and coagulation activation are highly reciprocal processes driven by multi-molecular interactions. Activated platelets secrete several coagulation factors and expose phosphatidylserine, which supports the activation of coagulation factor proteins. On the other hand, the coagulation cascade generates known ligands for platelet receptors, such as thrombin and fibrin. Coagulation factor (F)Xa, (F)XIIIa and activated protein C (APC) can also bind to platelets, but the functional consequences are unclear. Here, we investigated the effects of the activated (anti)coagulation factors on platelets, other than thrombin. Multicolor flow cytometry and aggregation experiments revealed that the ‘supernatant of (hirudin-treated) coagulated plasma’ (SCP) enhanced CRP-XL-induced platelet responses, i.e., integrin αIIbβ3 activation, P-selectin exposure and aggregate formation. We demonstrated that FXIIIa in combination with APC enhanced platelet activation in solution, and separately immobilized FXIIIa and APC resulted in platelet spreading. Platelet activation by FXIIIa was inhibited by molecular blockade of glycoprotein VI (GPVI) or Syk kinase. In contrast, platelet spreading on immobilized APC was inhibited by PAR1 blockade. Immobilized, but not soluble, FXIIIa and APC also enhanced in vitro adhesion and aggregation under flow. In conclusion, in coagulation, factors other than thrombin or fibrin can induce platelet activation via GPVI and PAR receptors. 相似文献
67.
68.
Amel Djoudi Rodolfo Molina-Pea Natalia Ferreira Ilaria Ottonelli Giovanni Tosi Emmanuel Garcion Frank Boury 《International journal of molecular sciences》2022,23(20)
Hyaluronic acid (HA) is a Glycosaminoglycan made of disaccharide units containing N-acetyl-D-glucosamine and glucuronic acid. Its molecular mass can reach 10 MDa and its physiological properties depend on its polymeric property, polyelectrolyte feature and viscous nature. HA is a ubiquitous compound found in almost all biological tissues and fluids. So far, HA grades are produced by biotechnology processes, while in the human organism it is a major component of the extracellular matrix (ECM) in brain tissue, synovial fluid, vitreous humor, cartilage and skin. Indeed, HA is capable of forming hydrogels, polymer crosslinked networks that are very hygroscopic. Based on these considerations, we propose an overview of HA-based scaffolds developed for brain cancer treatment, central and peripheral nervous systems, discuss their relevance and identify the most successful developed systems. 相似文献
69.
Marina Martello Vincenza Solli Rosalinda Termini Ajsi Kanapari Daniel Remondini Enrica Borsi Andrea Poletti Silvia Armuzzi Barbara Taurisano Ilaria Vigliotta Gaia Mazzocchetti Elena Zamagni Alessandra Merlotti Paola Tacchetti Lucia Pantani Serena Rocchi Ilaria Rizzello Katia Mancuso Michele Cavo Carolina Terragna 《International journal of molecular sciences》2022,23(20)
DNA microarrays and RNA-based sequencing approaches are considered important discovery tools in clinical medicine. However, cross-platform reproducibility studies undertaken so far have highlighted that microarrays are not able to accurately measure gene expression, particularly when they are expressed at low levels. Here, we consider the employment of a digital PCR assay (ddPCR) to validate a gene signature previously identified by gene expression profile. This signature included ten Hedgehog (HH) pathways’ genes able to stratify multiple myeloma (MM) patients according to their self-renewal status. Results show that the designed assay is able to validate gene expression data, both in a retrospective as well as in a prospective cohort. In addition, the plasma cells’ differentiation status determined by ddPCR was further confirmed by other techniques, such as flow cytometry, allowing the identification of patients with immature plasma cells’ phenotype (i.e., expressing CD19+/CD81+ markers) upregulating HH genes, as compared to others, whose plasma cells lose the expression of these markers and were more differentiated. To our knowledge, this is the first technical report of gene expression data validation by ddPCR instead of classical qPCR. This approach permitted the identification of a Maturation Index through the integration of molecular and phenotypic data, able to possibly define upfront the differentiation status of MM patients that would be clinically relevant in the future. 相似文献
70.
Rosso Ilaria Antonini Massimiliano Galletti Camilla Saracco Guido Specchia Vito 《Topics in Catalysis》2004,30(1-4):475-480
Topics in Catalysis - Ru-based catalysts supported on A zeolites and alumina were synthesised, characterised (XRD, SEM-EDS, TPR) and tested under realistic conditions for the preferential oxidation... 相似文献