全文获取类型
收费全文 | 472篇 |
免费 | 21篇 |
专业分类
电工技术 | 5篇 |
化学工业 | 227篇 |
金属工艺 | 5篇 |
机械仪表 | 4篇 |
建筑科学 | 17篇 |
矿业工程 | 2篇 |
能源动力 | 4篇 |
轻工业 | 71篇 |
水利工程 | 1篇 |
石油天然气 | 1篇 |
无线电 | 16篇 |
一般工业技术 | 78篇 |
冶金工业 | 11篇 |
原子能技术 | 2篇 |
自动化技术 | 49篇 |
出版年
2023年 | 11篇 |
2022年 | 70篇 |
2021年 | 64篇 |
2020年 | 25篇 |
2019年 | 16篇 |
2018年 | 21篇 |
2017年 | 18篇 |
2016年 | 19篇 |
2015年 | 21篇 |
2014年 | 17篇 |
2013年 | 33篇 |
2012年 | 27篇 |
2011年 | 43篇 |
2010年 | 21篇 |
2009年 | 15篇 |
2008年 | 21篇 |
2007年 | 16篇 |
2006年 | 9篇 |
2005年 | 5篇 |
2004年 | 4篇 |
2003年 | 4篇 |
2002年 | 3篇 |
2001年 | 2篇 |
1999年 | 2篇 |
1998年 | 2篇 |
1996年 | 1篇 |
1995年 | 2篇 |
1994年 | 1篇 |
排序方式: 共有493条查询结果,搜索用时 31 毫秒
71.
72.
Carmela De Simone Pasquale Ferranti Gianluca Picariello Ilaria Scognamiglio Alessandra Dicitore Francesco Addeo Lina Chianese Paola Stiuso 《Molecular nutrition & food research》2011,55(2):229-238
Scope: Milk proteins are a source of bioactive peptides. Recent studies have indicated that protein‐derived peptides released in buffalo cheese acid whey exert a cytomodulatory effect in human epithelial colon cancer (CaCo2) cells. The aim of the present study was to explain the molecular mechanism involved in the response of CaCo2 cells to oxidative stress in the presence of peptide fractions of buffalo cheese whey, purified and characterized by mass spectrometry. Methods and results: We demonstrated that treatment of CaCo2 treated with H2O2 (H‐CaCo2) cells with a partially purified peptide sub‐fraction (f3) from buffalo cheese acid whey induced a reduction of mitochondrial superoxide anion with subsequent decrease in heat shock protein 70 and 90 expression. Moreover, we observed a 5‐fold decrease in cyclin A expression and cell cycle arrest in G1/G0 phases. These responses were associated with increased activity of alkaline phosphatase and beta‐galactosidase, markers of differentiation and senescence respectively. Conclusions: The structural characterization of the active peptide fraction and the elucidation of the effects induced by its treatment on H‐CaCo2 cells in vitro demonstrated an activity of this peptide sub‐fraction in the modulation of cell cycle, thus suggesting potential application for the development of nutraceuticals as well as health‐promoting functional foods. 相似文献
73.
Ilaria Patruno Dr. Dawn Thompson Dr. Sergio Dall'Angelo Prof. Albert D. Windhorst Dr. Danielle J. Vugts Dr. Alex J. Poot Dr. Nimesh Mody Prof. Matteo Zanda 《ChemMedChem》2020,15(16):1579-1590
Fenretinide (4-HPR) is a synthetic derivative of all-trans-retinoic acid (ATRA) characterised by improved therapeutic properties and toxicological profile relative to ATRA. 4-HPR has been mostly investigated as an anti-cancer agent, but recent studies showed its promising therapeutic potential for preventing metabolic syndrome. Several biological targets are involved in 4-HPR's activity, leading to the potential use of this molecule for treating different pathologies. However, although 4-HPR displays quite well-understood multitarget promiscuity with regards to pharmacology, interpreting its precise physiological role remains challenging. In addition, despite promising results in vitro, the clinical efficacy of 4-HPR as a chemotherapeutic agent has not been satisfactory so far. Herein, we describe the preparation of a library of 4-HPR analogues, followed by the biological evaluation of their anti-cancer and anti-obesity/diabetic properties. The click-type analogue 3 b showed good capacity to reduce the amount of lipid accumulation in 3T3-L1 adipocytes during differentiation. Furthermore, it showed an IC50 of 0.53±0.8 μM in cell viability tests on breast cancer cell line MCF-7, together with a good selectivity (SI=121) over noncancerous HEK293 cells. Thus, 3 b was selected as a potential PET tracer to study retinoids in vivo, and the radiosynthesis of [18F] 3b was successfully developed. Unfortunately, the stability of [18F] 3b turned out to be insufficient to pursue imaging studies. 相似文献
74.
Elisabetta Coppi Ilaria Dettori Federica Cherchi Irene Bulli Martina Venturini Daniele Lana Maria Grazia Giovannini Felicita Pedata Anna Maria Pugliese 《International journal of molecular sciences》2020,21(24)
Adenosine is a signaling molecule, which, by activating its receptors, acts as an important player after cerebral ischemia. Here, we review data in the literature describing A2BR-mediated effects in models of cerebral ischemia obtained in vivo by the occlusion of the middle cerebral artery (MCAo) or in vitro by oxygen-glucose deprivation (OGD) in hippocampal slices. Adenosine plays an apparently contradictory role in this receptor subtype depending on whether it is activated on neuro-glial cells or peripheral blood vessels and/or inflammatory cells after ischemia. Indeed, A2BRs participate in the early glutamate-mediated excitotoxicity responsible for neuronal and synaptic loss in the CA1 hippocampus. On the contrary, later after ischemia, the same receptors have a protective role in tissue damage and functional impairments, reducing inflammatory cell infiltration and neuroinflammation by central and/or peripheral mechanisms. Of note, demyelination following brain ischemia, or autoimmune neuroinflammatory reactions, are also profoundly affected by A2BRs since they are expressed by oligodendroglia where their activation inhibits cell maturation and expression of myelin-related proteins. In conclusion, data in the literature indicate the A2BRs as putative therapeutic targets for the still unmet treatment of stroke or demyelinating diseases. 相似文献
75.
Francesco Morena Chiara Argentati Michelina Soccio Ilaria Bicchi Francesca Luzi Luigi Torre Andrea Munari Carla Emiliani Matteo Gigli Nadia Lotti Ilaria Armentano Sabata Martino 《International journal of molecular sciences》2020,21(23)
Herein, we present poly(butylene 1,4-cyclohexanedicarboxylate) (PBCE) films characterized by an unpatterned microstructure and a specific hydrophobicity, capable of boosting a drastic cytoskeleton architecture remodeling, culminating with the neuronal-like differentiation of human bone marrow-mesenchymal stem cells (hBM-MSCs). We have used two different filming procedures to prepare the films, solvent casting (PBCE) and compression-moulding (PBCE*). PBCE film had a rough and porous surface with spherulite-like aggregations (Ø = 10–20 μm) and was characterized by a water contact angle = 100°. PBCE* showed a smooth and continuous surface without voids and visible spherulite-like aggregations and was more hydrophobic (WCA = 110°). Both surface characteristics were modulated through the copolymerization of different amounts of ether-oxygen-containing co-units into PBCE chemical structure. We showed that only the surface characteristics of PBCE-solvent-casted films steered hBM-MSCs toward a neuronal-like differentiation. hBM-MSCs lost their canonical mesenchymal morphology, acquired a neuronal polarized shape with a long cell protrusion (≥150 μm), expressed neuron-specific class III β-tubulin and microtubule-associated protein 2 neuronal markers, while nestin, a marker of uncommitted stem cells, was drastically silenced. These events were observed as early as 2-days after cell seeding. Of note, the phenomenon was totally absent on PBCE* film, as hBM-MSCs maintained the mesenchymal shape and behavior and did not express neuronal/glial markers. 相似文献
76.
77.
Miriam Saponaro Isabella Giacomini Giulia Morandin Veronica Cocetta Eugenio Ragazzi Genny Orso Ilaria Carnevali Massimiliano Berretta Mariangela Mancini Francesco Pagano Monica Montopoli 《International journal of molecular sciences》2020,21(23)
Benign prostatic hyperplasia (BPH) is an age-related chronic disorder, characterized by the hyperproliferation of prostatic epithelial and stromal cells, which drives prostate enlargement. Since BPH aetiology and progression have been associated with the persistence of an inflammatory stimulus, induced both by Nuclear Factor-kappa B (NF-κB) activation and reactive oxygen species (ROS) production, the inhibition of these pathways could result in a good tool for its clinical treatment. This study aimed to evaluate the antioxidant and anti-inflammatory activity of a combined formulation of Serenoa repens and Urtica dioica (SR/UD) in an in vitro human model of BPH. The results confirmed both the antioxidant and the anti-inflammatory effects of SR/UD. In fact, SR/UD simultaneously reduced ROS production, NF-κB translocation inside the nucleus, and, consequently, interleukin 6 (IL-6) and interleukin 8 (IL-8) production. Furthermore, the effect of SR/UD was also tested in a human androgen-independent prostate cell model, PC3. SR/UD did not show any significant antioxidant and anti-inflammatory effect, but was able to reduce NF-κB translocation. Taken together, these results suggested a promising role of SR/UD in BPH and BPH-linked disorder prevention. 相似文献
78.
79.
Delahaye P Galata A Angot J Ban G Celona L Choinski J Gmaj P Jakubowski A Jardin P Kalvas T Koivisto H Kolhinen V Lamy T Lunney D Maunoury L Porcellato AM Prete GF Steckiewicz O Sortais P Thuillier T Tarvainen O Traykov E Varenne F Wenander F 《The Review of scientific instruments》2012,83(2):02A906
As the most ambitious concept of isotope separation on line (ISOL) facility, EURISOL aims at producing unprecedented intensities of post-accelerated radioactive isotopes. Charge breeding, which transforms the charge state of radioactive beams from 1+ to an n+ charge state prior to post-acceleration, is a key technology which has to overcome the following challenges: high charge states for high energies, efficiency, rapidity and purity. On the roadmap to EURISOL, a dedicated R&D is being undertaken to push forward the frontiers of the present state-of-the-art techniques which use either electron cyclotron resonance or electron beam ion sources. We describe here the guidelines of this R&D. 相似文献
80.
Fluorescent Diarylethene Photoswitches—A Universal Tool for Super‐Resolution Microscopy in Nanostructured Materials
下载免费PDF全文
![点击此处可从《Small (Weinheim an der Bergstrasse, Germany)》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Oleksii Nevskyi Dmytro Sysoiev Jes Dreier Simon Christoph Stein Alex Oppermann Florian Lemken Tobias Janke Jörg Enderlein Ilaria Testa Thomas Huhn Dominik Wöll 《Small (Weinheim an der Bergstrasse, Germany)》2018,14(10)
Super‐resolution fluorescence microscopy allows for unprecedented in situ visualization of biological structures, but its application to materials science has so far been comparatively limited. One of the main reasons is the lack of powerful dyes that allow for labeling and photoswitching in materials science systems. In this study it is shown that appropriate substitution of diarylethenes bearing a fluorescent closed and dark open form paves the way for imaging nanostructured materials with three of the most popular super‐resolution fluorescence microscopy methods that are based on different concepts to achieve imaging beyond the diffraction limit of light. The key to obtain optimal resolution lies in a proper control over the photochemistry of the photoswitches and its adaption to the system to be imaged. It is hoped that the present work will provide researchers with a guide to choose the best photoswitch derivative for super‐resolution microscopy in materials science, just like the correct choice of a Swiss Army Knife's tool is essential to fulfill a given task. 相似文献