全文获取类型
收费全文 | 476篇 |
免费 | 21篇 |
专业分类
电工技术 | 5篇 |
化学工业 | 227篇 |
金属工艺 | 5篇 |
机械仪表 | 3篇 |
建筑科学 | 17篇 |
矿业工程 | 2篇 |
能源动力 | 4篇 |
轻工业 | 68篇 |
水利工程 | 1篇 |
石油天然气 | 2篇 |
无线电 | 16篇 |
一般工业技术 | 79篇 |
冶金工业 | 17篇 |
原子能技术 | 2篇 |
自动化技术 | 49篇 |
出版年
2023年 | 11篇 |
2022年 | 70篇 |
2021年 | 63篇 |
2020年 | 25篇 |
2019年 | 15篇 |
2018年 | 21篇 |
2017年 | 18篇 |
2016年 | 19篇 |
2015年 | 21篇 |
2014年 | 17篇 |
2013年 | 34篇 |
2012年 | 25篇 |
2011年 | 43篇 |
2010年 | 22篇 |
2009年 | 15篇 |
2008年 | 21篇 |
2007年 | 16篇 |
2006年 | 9篇 |
2005年 | 5篇 |
2004年 | 4篇 |
2003年 | 4篇 |
2002年 | 3篇 |
2001年 | 2篇 |
1999年 | 2篇 |
1998年 | 3篇 |
1997年 | 1篇 |
1996年 | 3篇 |
1995年 | 2篇 |
1994年 | 2篇 |
1993年 | 1篇 |
排序方式: 共有497条查询结果,搜索用时 15 毫秒
101.
Manar Aoun Ilaria Passerini Pietro Chiurazzi Marianthi Karali Irene De Rienzo Giovanna Sartor Vittoria Murro Natalia Filimonova Marco Seri Sandro Banfi 《International journal of molecular sciences》2021,22(13)
Inherited retinal diseases (IRDs) are a heterogeneous group of conditions that include retinitis pigmentosa (RP) and Leber congenital amaurosis (LCA) and early-onset severe retinal dystrophy (EO[S]RD), which differ in severity and age of onset. IRDs are caused by mutations in >250 genes. Variants in the RPE65 gene account for 0.6–6% of RP and 3–16% of LCA/EORD cases. Voretigene neparvovec is a gene therapy approved for the treatment of patients with an autosomal recessive retinal dystrophy due to confirmed biallelic RPE65 variants (RPE65-IRDs). Therefore, the accurate molecular diagnosis of RPE65-IRDs is crucial to identify ‘actionable’ genotypes—i.e., genotypes that may benefit from the treatment—and is an integral part of patient management. To date, hundreds of RPE65 variants have been identified, some of which are classified as pathogenic or likely pathogenic, while the significance of others is yet to be established. In this review, we provide an overview of the genetic diagnostic workup needed to select patients that could be eligible for voretigene neparvovec treatment. Careful clinical characterization of patients by multidisciplinary teams of experts, combined with the availability of next-generation sequencing approaches, can accelerate patients’ access to available therapeutic options. 相似文献
102.
Andrea Z. Dalla Valle Ilaria Mignani Anna Spinardi Fabio Galvano Salvatore Ciappellano 《European Food Research and Technology》2007,225(2):167-172
Total polyphenols, carotenoids and vitamin C were assessed in three different peaches cultivars (Prunus persica): ‘Luisa Berselli’ (LB), ‘Stark Earlyglo’ (SE) and ‘Maria Serena’ (MS). Total antioxidant capacity of fruits and plasma antioxidant
activity after ingestion of fruits were determined as Trolox equivalent antioxidant capacity (TEAC) and as plasma total radical-trapping
potential (TRAP) respectively. The amount of polyphenols, carotenoids and vitamin C resulted to be generally similar in the
three cultivars. TEAC, measured from harvest to 7 days postharvest, resulted to be influenced mainly by vitamin C content,
whereas polyphenols and carotenoids seemed to play a secondary role. Although TEAC was similar in the three cultivars, only
LB significantly increased the TRAP in human plasma at 1, 2 and 4 h after ingestion of peaches. Sugar moiety, condensed and
glycoside phenols are suggested to be involved in the higher effect on plasma TRAP of LB. 相似文献
103.
Fratoddi I Venditti I Battocchio C Polzonetti G Cametti C Russo MV 《Nanoscale research letters》2011,6(1):98
Noble metal nanoparticles of different sizes and shapes combined with conjugated functional polymers give rise to advanced
core shell hybrids with interesting physical characteristics and potential applications in sensors or cancer therapy. In this
paper, a versatile and facile synthesis of core shell systems based on noble metal nanoparticles (AuNPs, AgNPs, PtNPs), coated
by copolymers belonging to the class of substituted polyacetylenes has been developed. The polymeric shells containing functionalities
such as phenyl, ammonium, or thiol pending groups have been chosen in order to tune hydrophilic and hydrophobic properties
and solubility of the target core shell hybrids. The Au, Ag, or Pt nanoparticles coated by poly(dimethylpropargylamonium chloride),
or poly(phenylacetylene-co-allylmercaptan). The chemical structure of polymeric shell, size and size distribution and optical
properties of hybrids have been assessed. The mean diameter of the metal core has been measured (about 10-30 nm) with polymeric
shell of about 2 nm. 相似文献
104.
105.
Mariotti Ilaria Barzotto Mariachiara Cor Giancarlo Saloriani Stefano 《The Annals of Regional Science》2020,64(3):523-546
The Annals of Regional Science - The present paper aims at exploring the location behaviour of manufacturing firms, according to their ownership: domestic firms (henceforth DOMs) and inward foreign... 相似文献
106.
Dr. Cristina Tintori Ilaria Laurenzana Francesco La Rocca Dr. Federico Falchi Prof. Fabio Carraro Alba Ruiz Prof. José A. Esté Miroslava Kissova Dr. Emmanuele Crespan Prof. Giovanni Maga Prof. Mariangela Biava Dr. Chiara Brullo Prof. Silvia Schenone Prof. Maurizio Botta 《ChemMedChem》2013,8(8):1353-1360
Hematopoietic cell kinase (Hck) is a member of the Src family of non‐receptor protein tyrosine kinases. High levels of Hck are associated with drug resistance in chronic myeloid leukemia. Furthermore, Hck activity has been connected with HIV‐1. Herein, structure‐based drug design efforts were aimed at identifying novel Hck inhibitors. First, an in‐house library of pyrazolo[3,4‐d]pyrimidine derivatives, which were previously shown to be dual Abl and c‐Src inhibitors, was analyzed by docking studies within the ATP binding site of Hck to select the best candidates to be tested in a cell‐free assay. Next, the same computational protocol was applied to screen a database of commercially available compounds. As a result, most of the selected compounds were found active against Hck, with Ki values ranging from 0.14 to 18.4 μM , confirming the suitability of the computational approach adopted. Furthermore, selected compounds showed an interesting antiproliferative activity profile against the human leukemia cell line KU‐812, and one compound was found to block HIV‐1 replication at sub‐toxic concentrations. 相似文献
107.
Kotoni D Ciogli A Molinaro C D'Acquarica I Kocergin J Szczerba T Ritchie H Villani C Gasparrini F 《Analytical chemistry》2012,84(15):6805-6813
A new chiral stationary phase for ultrahigh-pressure liquid chromatography (UHPLC) applications was prepared by covalent attachment of the Whelk-O1 selector to spherical, high-surface-area 1.7-μm porous silica particles. Columns of varying dimensions (lengths of 50, 75, 100, and 150 mm and internal diameters of 3.0 or 4.6 mm) were packed and characterized in terms of permeability, efficiency, retention, and enantioselectivity, using both organic and water-rich mobile phases. A conventional HPLC Whelk-O1 column based on 5.0-μm porous silica particles and packed in a 250 mm × 4.6 mm column was used as a reference. Van Deemter curves, generated with low-molecular-weight solutes on a 100 mm × 4.6 mm column packed with the 1.7-μm particles, showed H(min) (μm) and μ(opt) (mm/s) values of 4.10 and 5.22 under normal-phase and 3.74 and 4.34 under reversed-phase elution conditions. The flat C term of the van Deemter curves observed with the 1.7-μm particles allowed the use of higher-than-optimal flow rates without significant efficiency loss. Kinetic plots constructed from van Deemter data confirmed the ability of the column packed with the 1.7-μm particles to afford subminute separations with good efficiency and its superior performances in the high-speed regime, compared to the column packed with 5.0-μm particles. Resolutions in the time scale of seconds were obtained using a 50-mm-long column in the normal phase or polar organic mode. The intrinsic kinetic performances of 1.7-μm silica particles are retained in the Whelk-O1 chiral stationary phase, clearly demonstrating the potentials of enantioselective UHPLC in terms of high speed, throughput, and resolution. 相似文献
108.
109.
Ilaria Saltarella Concetta Altamura Aurelia Lamanuzzi Benedetta Apollonio Angelo Vacca Maria Antonia Frassanito Jean-Franois Desaphy 《International journal of molecular sciences》2022,23(13)
Ion channels are pore-forming proteins that allow ions to flow across plasma membranes and intracellular organelles in both excitable and non-excitable cells. They are involved in the regulation of several biological processes (i.e., proliferation, cell volume and shape, differentiation, migration, and apoptosis). Recently, the aberrant expression of ion channels has emerged as an important step of malignant transformation, tumor progression, and drug resistance, leading to the idea of “onco-channelopathy”. Here, we review the contribution of ion channels and transporters in multiple myeloma (MM), a hematological neoplasia characterized by the expansion of tumor plasma cells (MM cells) in the bone marrow (BM). Deregulation of ion channels sustains MM progression by modulating intracellular pathways that promote MM cells’ survival, proliferation, and drug resistance. Finally, we focus on the promising role of ion channels as therapeutic targets for the treatment of MM patients in a combination strategy with currently used anti-MM drugs to improve their cytotoxic activity and reduce adverse effects. 相似文献