Thermodynamic properties of malonic, succinic, and glutaric acids: evaporation rates and saturation vapor pressures

This work provides thermodynamic data, in particular, liquid-state saturation vapor pressures of three common slightly water soluble secondary organic aerosol components, namely, malonic, succinic, and glutaric acids. A modified tandem differential mobility analyzer system was used to measure evaporation rates of nanometer sized aqueous malonic, succinic, and glutaric acid droplets at relative humidities and temperatures relevant in the lower troposphere. Liquid phase saturation vapor pressures and other thermodynamic properties were derived from the measurements using a binary condensation model. The obtained expressions for liquid phase saturation vapor pressures compare well with extrapolated literature data. The importance of the choice of method for calculating activity coefficients is discussed.     

Effect of the oat,horse chestnut,cowherb, soy,quinoa and soapwort extracts on skin-mimicking monolayers and cell lines

The main goal of the study was to compare the effect of aqueous extracts of oat (Avena sativa L.), horse chestnut (Aesculus hippocastanum L.), quinoa (Chenopodium quinoa Willd.), soapwort (Saponaria officinalis L.), cowherb (Vaccaria hispanica [P. Mill.] Rauschert) and soy (Glycine max L.) on model lipid monolayers mimicking the lipid membrane of keratinocytes and intercellular lipids of stratum corneum, and on human skin-related cell lines. Two lipid monolayers, consisting of a dipalmitoylphosphatidylcholine (DPPC) and cholesterol mixture in a molar ratio of 7:3 and Ceramide VI, stearic acid and cholesterol in a molar ratio of 1:1:0.7, and two cell lines (human keratinocyte HaCaT and human skin malignant melanoma A375) were employed. None of the extracts reduced surface pressure below the level achieved for bare monolayers. The strength of the effect on the lipid monolayers (horse chestnut > cowherb > soapwort > soy) points to the existence of some specific interactions responsible for the observed affinity of biosurfactants from the extracts to the lipids in the monolayers. The cytotoxicity tests performed with two model skin cell lines showed that all six plants extracts significantly reduced the cells' viability in a concentration-dependent way. The model lipid monolayers were not solubilized by the investigated surface-active extracts. The latter thus proved interesting candidates for application in mild cleansing cosmetic formulations. Penetration of the monolayers by surface-active components of some extracts, especially horse chestnut, cowherb and soapwort, opens new possibilities for topical delivery of active components.     

Wall effects in smog chamber experiments: A model study

Wall losses of condensable organic vapors are a significant complication for smog-chamber experiments designed to constrain production of Secondary Organic Aerosols (SOA). Here we develop a dynamical mass-balance model based on the Volatility Basis Set (VBS) to explore various pathways for mass transfer between the bulk of a smog-chamber volume (the vapors and suspended particles) and reservoirs near the chamber walls (deposited and/or nucleated particles on the walls, adsorption to the walls, and sorption into Teflon walls). We consider various limiting cases and explore the sensitivity of inferred SOA yields to assumptions about the actual parameters in a given SOA experiment. We also present data suggesting that adsorptive uptake to Teflon for typical SOA is modest. Broadly, we find that walls become a sink for condensable vapors when those vapors interact with either deposited particles of the Teflon walls, with qualitatively similar effects on the suspended particles. Finally, we show that having a relatively high seed condensation sink is vital to reliable chamber mass balances.

Copyright © 2016 American Association for Aerosol Research     

ManyLands: A Journey Across 4D Phase Space of Trajectories

Mathematical models of ordinary differential equations are used to describe and understand biological phenomena. These models are dynamical systems that often describe the time evolution of more than three variables, i.e., their dynamics take place in a multi‐dimensional space, called the phase space. Currently, mathematical domain scientists use plots of typical trajectories in the phase space to analyze the qualitative behavior of dynamical systems. These plots are called phase portraits and they perform well for 2D and 3D dynamical systems. However, for 4D, the visual exploration of trajectories becomes challenging, as simple subspace juxtaposition is not sufficient. We propose ManyLands to support mathematical domain scientists in analyzing 4D models of biological systems. By describing the subspaces as Lands, we accompany domain scientists along a continuous journey through 4D HyperLand, 3D SpaceLand, and 2D FlatLand, using seamless transitions. The Lands are also linked to 1D TimeLines. We offer an additional dissected view of trajectories that relies on small‐multiple compass‐alike pictograms for easy navigation across subspaces and trajectory segments of interest. We show three use cases of 4D dynamical systems from cell biology and biochemistry. An informal evaluation with mathematical experts confirmed that ManyLands helps them to visualize and analyze complex 4D dynamics, while facilitating mathematical experiments and simulations.     

General conditions for online estimation and optimization of reliability characteristics

A novel approach to estimate reliability properties of systems or components individually during operation is presented. It is distinguished between slow and fast reliability states based on an equivalent system representation. Conditions for their observability and control are given and objectives for optimal reliability-based control are discussed in general.     

The role of drinking in the suppression of food intake by recent activity.

The standard activity-based anorexia procedure provides rats with access to a running wheel while restricting their access to dry food. This can produce reduced food intake and progressive weight loss. Using this procedure, in the present study (Experiment 1) the authors found changes in drinking patterns both in the period of high activity preceding food access and during the feeding period. Varying the procedure by providing wet mash (Experiment 2) or by prior adaptation to a drinking schedule (Experiment 3) prevented the self-starvation effect. These results indicate the importance of drinking when analyzing the effect of recent activity on food intake. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Modulation of Inflammatory Cytokine Production in Human Monocytes by cGMP and IRAK3

Interleukin-1 receptor-associated kinase-3 (IRAK3) is a critical checkpoint molecule of inflammatory responses in the innate immune system. The pseudokinase domain of IRAK3 contains a guanylate cyclase (GC) centre that generates small amounts of cyclic guanosine monophosphate (cGMP) associated with IRAK3 functions in inflammation. However, the mechanisms of IRAK3 actions are poorly understood. The effects of low cGMP levels on inflammation are unknown, therefore a dose–response effect of cGMP on inflammatory markers was assessed in THP-1 monocytes challenged with lipopolysaccharide (LPS). Sub-nanomolar concentrations of membrane permeable 8-Br-cGMP reduced LPS-induced NFκB activity, IL-6 and TNF-α cytokine levels. Pharmacologically upregulating cellular cGMP levels using a nitric oxide donor reduced cytokine secretion. Downregulating cellular cGMP using a soluble GC inhibitor increased cytokine levels. Knocking down IRAK3 in THP-1 cells revealed that unlike the wild type cells, 8-Br-cGMP did not suppress inflammatory responses. Complementation of IRAK3 knockdown cells with wild type IRAK3 suppressed cytokine production while complementation with an IRAK3 mutant at GC centre only partially restored this function. Together these findings indicate low levels of cGMP form a critical component in suppressing cytokine production and in mediating IRAK3 action, and this may be via a cGMP enriched nanodomain formed by IRAK3 itself.     

Molecular and structural characteristics of cod gelatin films modified with EDC and TGase

Cod gelatin films before and after cross-linking of gelatin with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) or transglutaminase (TGase) have been characterized by Fourier transform infrared (FT-IR) spectroscopy and differential scanning calorimetry (DSC) analysis. For comparison, a film prepared from unmodified pig gelatin has been also analysed. The difference spectra showed that cod gelatin during the film formation involved first of all water-to-amide hydrogen bonds, and the film from pig gelatin contained water-to-amide, amide-to-amide and water-to-water hydrogen bonds. A higher number of hydrogen bonds in the structure of the film from pig gelatin contributed to much better recovering of the helical structure in this film than in the film from cod gelatin, as the peaks at about 1663 cm^?1 in the amide I band and at about 1537 cm^?1 in the amide II band in the second-derivative spectra revealed. The recovered helical structure, in turn, resulted in a significantly higher melting enthalpy value in the case of the film from the pig gelatin. After modification of cod gelatin with EDC or TGase, the inter-chain cross-linkages formed in the films led to the conformation of gelatin with no indications of helical ordering. An increase of melting temperature of gelatin films by 7 °C on EDC and by 10 °C on the TGase modifications was related to the formation of covalent cross-links, and a decrease of glass temperature by 28 °C and 7 °C on EDC and TGase cross-linking, respectively, demonstrated the plasticizing effect of water.     

The use of intensity-dependent weight functions to “Weberize” $$L^2$$ L 2 -based methods of signal and image approximation

Optimization and Engineering - We consider the problem of modifying $$L^2$$ -based approximations so that they “conform” in a better way to Weber’s model of perception: Given a...