Wind tunnel study and numerical simulation of dust particle resuspension from indoor surfaces in turbulent flows

Particle resuspension from flooring is an important source of air pollution in the indoor environment. In this work, resuspension of monolayer, polydisperse, irregularly shaped dust particles from various types of floorings was studied via a series of wind tunnel experiments. The range of free-stream velocity needed for resuspension of dust particles was evaluated as a function of particle size and material of particles and surfaces. In addition, a Monte Carlo simulation for predicting the resuspension of dust particles was developed. The resuspension model took into account the effects of particle irregularity, particle surface roughness, and flow characteristics. The dust particle resuspension from different floorings for several particle sizes was evaluated. The model predictions for resuspension fractions were compared with the experimental data and good agreement was observed. The study provided information on the role of airflow velocity on irregular dust particle resuspension from common floorings.     

Acetate and phosphate anion adsorption linear sweep voltammograms simulated using density functional theory

Specific adsorption of anions to electrode surfaces may alter the rates of electrocatalytic reactions. Density functional theory (DFT) methods are used to predict the adsorption free energy of acetate and phosphate anions as a function of Pt(1 1 1) electrode potential. Four models of the electrode potential are used including a simple vacuum slab model, an applied electric field model with and without the inclusion of a solvating water bi-layer, and the double reference model. The linear sweep voltammogram (LSV) due to anion adsorption is simulated using the DFT results. The inclusion of solvation at the electrochemical interface is necessary for accurately predicting the adsorption peak position. The Langmuir model is sufficient for predicting the adsorption peak shape, indicating coverage effects are minor in altering the LSV for acetate and phosphate adsorption. Anion adsorption peak positions are determined for solution phase anion concentrations present in microbial fuel cells and microbial electrolysis cells and discussion is provided as to the impact of anion adsorption on oxygen reduction and hydrogen evolution reaction rates in these devices.     

Reflected shock tube experiments on aeroacoustic signature of hot jets

Journal of Mechanical Science and Technology - We used a reflected shock tube to investigate the acoustic signature of a hot jet at the far-field. Experiments were performed at Mach = 1.4 and a...     

Endosomal Escape of Polymer‐Coated Silica Nanoparticles in Endothelial Cells

Current investigations into hazardous nanoparticles (i.e., nanotoxicology) aim to understand the working mechanisms that drive toxicity. This understanding has been used to predict the biological impact of the nanocarriers as a function of their synthesis, material composition, and physicochemical characteristics. It is particularly critical to characterize the events that immediately follow cell stress resulting from nanoparticle internalization. While reactive oxygen species and activation of autophagy are universally recognized as mechanisms of nanotoxicity, the progression of these phenomena during cell recovery has yet to be comprehensively evaluated. Herein, primary human endothelial cells are exposed to controlled concentrations of polymer‐functionalized silica nanoparticles to induce lysosomal damage and achieve cytosolic delivery. In this model, the recovery of cell functions lost following endosomal escape is primarily represented by changes in cell distribution and the subsequent partitioning of particles into dividing cells. Furthermore, multilamellar bodies are found to accumulate around the particles, demonstrating progressive endosomal escape. This work provides a set of biological parameters that can be used to assess cell stress related to nanoparticle exposure and the subsequent recovery of cell processes as a function of endosomal escape.     

Applying simulated annealing for designing cellular manufacturing systems using MDmTSP

The design of cellular manufacturing systems involves many structural and operational issues. One of the important design steps is the formation of part families and machine cells (cell formation). Despite a large number of papers on cell formation published worldwide, only a handful incorporates operation sequence in layout design (intra-cell move calculations). We propose a solution to solve the part-family and machine-cell formation problem considering the within-cell layout problem, simultaneously. In this paper, the cellular manufacturing system is formulated as a multiple departures single destination multiple travelling salesman problem (MDmTSP) and a solution methodology based on simulated annealing is proposed to solve the formulated model. Numerical examples show that the proposed method is efficient and effective in finding optimal solutions. The results also indicate that the proposed approach performs well compared to some well-known cell formation methods.     

Microstructured silicon membrane with soft suspension beams for a high performance MEMS microspeaker

This study presents a novel approach to MEMS microspeakers design aiming to tackle two main drawbacks of conventional microspeakers: their poor sound quality and their weak efficiency. For this purpose, an acoustic emissive surface based on a very light but very stiff structured silicon membrane was designed and microfabricated. This architecture, for which the membrane undesirable vibration modes were reduced to only three within the microspeaker bandwidth, is promising to let the microspeaker produce high sound quality from 300?Hz to 20?kHz. This silicon membrane is suspended by a whole set of silicon springs designed to enable out-of-plane displacements as large as 300?μm. Different geometries of springs were considered and the material maximum stress was analyzed in each case by finite element modeling. The proposed structure promises an efficiency of 10^?4, that is to say ten times higher than that of conventional microspeakers.     

Effect of partial replacement of chitosan with halloysite nanotubes on the properties of polylactic acid hybrid biocomposites

In this study, hybrid chitosan/halloysite nanotubes (Cs/HNTs) reinforced polylactic acid (PLA) were prepared via melt compounding and compression molding techniques. In the fabrication of PLA/Cs/HNTs hybrid biocomposites, the partial replacement of Cs with HNTs was performed at filler loading of 2.5 parts per hundred parts of polymer (php), proceeding from the highest tensile strength of PLA/Cs obtained in our previous study. Cs was partially replaced with different HNTs loadings (0.5, 1, 1.5, 2, and 2.5) php and its effects on the functional group, thermal, tensile, morphological, and water absorption properties were investigated systematically. The results revealed that the combined loading of 1 php Cs and 1.5 php HNTs hybrid fillers into PLA showed the best performance in all properties. Fourier transform infrared spectroscopy (FTIR) analysis indicated that the siloxane (Si O) group of HNTs had chemically interacted with the amine group of Cs. The thermal analysis demonstrated that partial replacement of Cs with 1.5 php HNTs improved the thermal stability of PLA/2.5Cs/0HNTs biocomposite by ~12%. Yet, the percentage of crystallinity (χ_c) reduced with HNTs addition due to the phase adhesion improvement. Moreover, PLA/1Cs/1.5HNTs hybrid biocomposites showed the highest tensile strength and elongation at break of 59 MPa and 2.72%, respectively. This correlated with the uniform dispersion and better interfacial adhesion between Cs/HNTs fillers in the PLA matrix, as confirmed by the field emission scanning electron microscopy (FESEM). In addition, partial replacement of Cs with HNTs exhibited a lower water absorption percentage, which suggested the advantage of hybrid fillers to reduce water uptake, and is beneficial in a wide range of applications.     

Integrative Study of the Structural and Dynamical Properties of a KirBac3.1 Mutant: Functional Implication of a Highly Conserved Tryptophan in the Transmembrane Domain

ATP-sensitive potassium (K-ATP) channels are ubiquitously expressed on the plasma membrane of cells in several organs, including the heart, pancreas, and brain, and they govern a wide range of physiological processes. In pancreatic β-cells, K-ATP channels composed of Kir6.2 and SUR1 play a key role in coupling blood glucose and insulin secretion. A tryptophan residue located at the cytosolic end of the transmembrane helix is highly conserved in eukaryote and prokaryote Kir channels. Any mutation on this amino acid causes a gain of function and neonatal diabetes mellitus. In this study, we have investigated the effect of mutation on this highly conserved residue on a KirBac channel (prokaryotic homolog of mammalian Kir6.2). We provide the crystal structure of the mutant KirBac3.1 W46R (equivalent to W68R in Kir6.2) and its conformational flexibility properties using HDX-MS. In addition, the detailed dynamical view of the mutant during the gating was investigated using the in silico method. Finally, functional assays have been performed. A comparison of important structural determinants for the gating mechanism between the wild type KirBac and the mutant W46R suggests interesting structural and dynamical clues and a mechanism of action of the mutation that leads to the gain of function.