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排序方式: 共有282条查询结果,搜索用时 156 毫秒
111.
Mixtures based on azomethine, imidazobenzoimidazole, and pyrimidobenzoimidazole derivatives were tested as inhibitors of iron corrosion in 1 M H2SO4 in the presence of 0.25 mol/l NaCl. The number and concentrations of their components were varied with the aim of studying their influence on the protection effects of the mixtures. The resulting experimental dependences, of the protective effectiveness on the number and concentration of the components, as well as the polar properties of substituents in their molecules, can be interpreted in terms of the linear Gibbs energy relation (LGER). This conclusion is valid over a wide range of medium temperatures (20–60°C), provided the volume concentrations of compounds in each reaction series are kept constant. 相似文献
112.
113.
Gudrun Schmidt-Naake Anne Schmalfuß Inga Woecht 《Chemical Engineering Research and Design》2008,86(7):765-6
The influence of the ionic liquid (IL) 1-ethyl-3-methylimidazoliumethylsulfate ([EMIM]EtSO4) on the polymerization kinetics of methyl methacrylate was investigated. ILs are liquids with relatively high polarities and viscosities. These two characteristic properties are strongly correlated with the rate coefficients of propagation kp and termination kt of polymerizations carried out in ILs. The rate constant of termination kt decreases when the concentration of ionic liquid, and thus the viscosity is increased, whereas the propagation rate coefficient kp increases with increasing IL content. The viscosity of ILs can be varied by either working with mixtures of ILs with conventional organic solvents – here the IL [EMIM]EtSO4 was mixed with dimethyl formamide (DMF) – or by variation of the temperature. The studies were carried out to determine the influence of the viscosity on the propagation and the termination reaction as well as the molecular weight distribution. 相似文献
114.
Barton SJ Richardson S Perkins DN Bellahn I Bryant TN Whittaker JC 《Analytical chemistry》2007,79(15):5601-5607
A database of 5448 peptide tandem mass spectra acquired in a quadrupole time-of-flight mass spectrometer was generated for peptides derived from proteins digested with trypsin. Peptides were identified from their mass spectra by the Mascot algorithm. Statistical models were then used to investigate factors influencing the abundance of ions formed. Separate models were formulated for b and y ions as it was thought that different factors may influence the formation of each type of ion. Several factors were found to have a highly significant influence on the abundance of ions formed. These include the actual mass of the ion formed after fragmentation as well as the location of the cleavage. The composition of the fragmenting peptide was also found to be important, and amino acids either side of the fragmentation site influenced the abundance of ions produced. To increase understanding of fragmentation mechanisms, the effect of several physicochemical properties of these residues was also investigated in a separate model. In conclusion, the models formulated for b and y ions provide useful characterization of the abundance of ions formed, and this information could be used to develop improved algorithms for peptide identification. 相似文献
115.
116.
Co-Stimulation of Oxytocin and Arginine-Vasopressin Receptors Affect Hypothalamic Neurospheroid Size
Mohammad Saied Salehi Inga D. Neumann Benjamin Jurek Sareh Pandamooz 《International journal of molecular sciences》2021,22(16)
Oxytocin (OXT) is a neuropeptide involved in a plethora of behavioral and physiological processes. However, there is a prominent lack of 3D cell culture models that investigate the effects of OXT on a cellular/molecular level. In this study, we established a hypothalamic neuronal spheroid model to investigate the cellular response in a more realistic 3D setting. Our data indicate that the formation of spheroids itself does not alter the basic characteristics of the cell line and that markers of cellular morphology and connectivity are stably expressed. We found that both OXT and arginine vasopressin (AVP) treatment increase spheroid size (surface area and volume), as well as individual nucleus size, which serves as an indicator for cellular proliferation. The cellular response to both OXT and AVP seems mainly to be mediated by the AVP receptor 1a (V1aR); however, the OXT receptor (OXTR) contributes significantly to the observed proliferative effect. When we blocked the OXTR pharmacologically or knocked down the OXTR by siRNA, the OXT- or AVP-induced cellular proliferation decreased. In summary, we established a 3D cell culture model of the neuronal response to OXT and AVP and found that spheroids react to the treatment via their respective receptors but also via cross-talk between the two receptor types. 相似文献
117.
Asfree Gwanyanya Inga Andriul Bogdan M. Istrate Farjana Easmin Kanigula Mubagwa Regina Ma
ianskien 《International journal of molecular sciences》2021,22(16)
The cardiac Mg2+-sensitive, TRPM6, and TRPM7-like channels remain undefined, especially with the uncertainty regarding TRPM6 expression in cardiomyocytes. Additionally, their contribution to the cardiac action potential (AP) profile is unclear. Immunofluorescence assays showed the expression of the TRPM6 and TRPM7 proteins in isolated pig atrial and ventricular cardiomyocytes, of which the expression was modulated by incubation in extracellular divalent cation-free conditions. In patch clamp studies of cells dialyzed with solutions containing zero intracellular Mg2+ concentration ([Mg2+]i) to activate the Mg2+-sensitive channels, raising extracellular [Mg2+] ([Mg2+]o) from the 0.9-mM baseline to 7.2 mM prolonged the AP duration (APD). In contrast, no such effect was observed in cells dialyzed with physiological [Mg2+]i. Under voltage clamp, in cells dialyzed with zero [Mg2+]i, depolarizing ramps induced an outward-rectifying current, which was suppressed by raising [Mg2+]o and was absent in cells dialyzed with physiological [Mg2+]i. In cells dialyzed with physiological [Mg2+]i, raising [Mg2+]o decreased the L-type Ca2+ current and the total delayed-rectifier current but had no effect on the APD. These results suggest a co-expression of the TRPM6 and TRPM7 proteins in cardiomyocytes, which are therefore the molecular candidates for the native cardiac Mg2+-sensitive channels, and also suggest that the cardiac Mg2+-sensitive current shortens the APD, with potential implications in arrhythmogenesis. 相似文献
118.
Lactobacilli isolated from the Armenian fermented milk product matsoun: Growth properties,antibacterial and proteolytic activity and their dependence on pH 下载免费PDF全文
Andranik Keryan Inga Bazukyan Armen Trchounian 《International Journal of Dairy Technology》2017,70(3):389-398
Growth, antibacterial and proteolytic activities of two new lactobacilli strains isolated from matsoun (Armenian traditional dairy product) and their pH dependence were studied. The results demonstrated the antibacterial activity of lactobacilli against Gram‐positive and Gram‐negative test strains. This activity was stable when pH of cell culture medium was adjusted to the value of 6.5. At pH 8, the antibacterial activity of only one strain was stable. Both strains were able to hydrolyse casein in pH range of 5.5–8 with maximal activity at pH 5.5. Controlled pH conditions were suitable for biomass yield, while noncontrolled pH was better for expression of antibacterial activity. 相似文献
119.
Kristin Fløgstad Degnes Hans Fredrik Nyvold Kvitvang Hanne Haslene-Hox Inga Marie Aasen 《Food and Bioprocess Technology》2017,10(6):1122-1130
Ripening of dry cured ham involves a high number of complex enzymatic and chemical reactions. Due to long processing time, there is a need for analytical methods that can be applied to monitor the ripening in optimization of process conditions and development of new products. In this study, nontargeted metabolite analysis by mass spectrometry (MS) was employed to determine the ripening rates at different water activities in small-scale laboratory experiments and to follow the progress of the ripening of hams from a dry cured ham production facility. Approximately 1000 metabolites were detected. Of these, 90–95% had molecular masses below 800 Da, and more than 60% below 500 Da. In the order of 150 metabolites were putatively annotated. In addition to free amino acids and muscle metabolites, the nontargeted analysis revealed the time profiles of di- and tripeptides, as well as a high number of compounds generated by further conversion of amino acids, muscle metabolites and probably lipids. Statistical processing of the data sets showed that the metabolite profiles changed with the storage time, and that ripening of fresh and unsalted, dried meat, generated other profiles than salted samples. In conclusion, our approach represents a simple and efficient tool for comparison of process conditions and to follow the time course of the ripening, useful in product development and process optimization. 相似文献
120.
Galina Vladimirovna Kontsevaya Ludmila Alekseevna Gerlinskaya Yury Mikhailovich Moshkin Margarita Vladimirovna Anisimova Aliya Konstantinovna Stanova Tatyana Ivanovna Babochkina Mikhail Pavlovich Moshkin 《International journal of molecular sciences》2021,22(19)
The latest vaccination campaign has actualized the potential impact of antigenic stimuli on reproductive functions. To address this, we mimicked vaccination’s effects by administering keyhole limpet hemocyanin (KLH ) to CD1 male mice and used their sperm for in vitro fertilization (IVF). Two-cell embryos after IVF with spermatozoa from control (C) or KLH-treated (Im) male mice were transferred to surrogate mothers mated with vasectomized control (C) or KLH-treated (Im) male mice, resulting in four experimental groups: C–C, Im–C, C–Im, and Im–Im. The pre-implantation losses were significantly lower in the Im–C group than in the C–Im group. At the same time, the resorption rates reduced markedly in the C–Im compared to the Im–C group. Embryo and placenta weights were significantly higher in the Im–Im group. Although the GM-CSF levels were lower in the amniotic fluid of the gestating surrogate mothers in the Im–Im group, they were strongly correlated with embryo mass. The number–size trade-off was only significant in the Im–Im group. This suggests a positive, cooperative effect of spermatozoa and seminal fluid from immune-primed males on embryo growth and the optimal distribution of surrogate mother maternal resources despite the negative impact of males’ antigenic challenge on the IVF success rate. 相似文献