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41.
Decsi T Boehm G Tjoonk HM Molnár S Dijck-Brouwer DA Hadders-Algra M Martini IA Muskiet FA Boersma ER 《Lipids》2002,37(10):959-965
Long-chain PUFA play an important role in early human neurodevelopment. Significant inverse correlations were reported between
values of trans isomeric and long-chain PUFA in plasma lipids of preterm infants and children aged 1–15 yr as well as in venous cord blood
lipids of full-term infants. Here we report FA compositional data of cord blood vessel wall lipids in 308 healthy, full-term
infants (gestational age: 39.7±1.2 wk, birth weight: 3528±429 g, mean±SD). The median (interquartile range) of the sum of
18-carbon trans FA was 0.22 (0.13)% w/w in umbilical artery and 0.16 (0.10)% w/w in umbilical vein lipids. Nonparametric correlation analysis
showed significant inverse correlations between the sum of 18-carbon trans FA and both arachidonic acid and DHA in artery (r=−0.38, P<0.01, and r=−0.20, P<0.01) and vein (r=−0.36, P<0.01, and −0.17, P<0.01) wall lipids. In addition, the sum of 18-carbon trans FA was significantly positively correlated to Mead acid, a general indicator of EFA deficiency, in both artery (r=+0.35, P<0.01) and vein (r=+0.31, P<0.01) wall lipids. The present results obtained in a large group of full-term infants suggest that maternal trans FA intake is inversely associated with long-chain PUFA status of the infant at birth. 相似文献
42.
A self-contained, microelectrochemical immunoassay on the smallest volumes reported to date (1 microL for the antigen, 1 microL for the secondary antibody-enzyme conjugate, and 200 nL for the electrochemically detected species) has been developed using mouse IgG as a model system in a sandwich-type enzyme-linked immunosorbant assay, which takes less than 30 min to both complete the assembly of immunoassay components onto the antibody-modified surface and detect enzymatically generated species (excluding time for electrochemical cleaning of electrodes). These studies demonstrate the advantage of the close proximity of electrodes to modified surfaces and their application in the analysis of small volumes. Using a 50 microm diameter x 8 microm deep cavity with individually addressable electrodes on a microfabricated chip, the primary antibody was selectively and covalently attached at a gold, recessed microdisk (RMD) at the bottom of the microcavity to the free end of SAMs of either 11-mercaptoundecanoic acid or 11-mercapto-1-undecanol using 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide hydrochloride. Nonspecific adsorption to the surrounding material, polyimide, of the microcavity device was eliminated. Electrochemical desorption was used to confine the immunoassay activity at the RMD. Enzymatic conversion of the substrate p-aminophenyl phosphate top-aminophenol is detectable in less than 30 s using cyclic voltammetry at a gold, tubular nanoband electrode, which is on the wall of the microcavity and immediately adjacent to the modified RMD. A third electrode, also within the region of the microcavity, served as the pseudoreference/auxiliary electrode. Calibration curves obtained for 1-microL solutions of 5-100 ng/mL of IgG and for 200 nL-solutions of 5 microM to 4 mM of PAPR gave detection limits of 4.4 nM (6.4 ng/mL) or 880 fmol (129 pg) for PAPR and 56 fM (9 pg/mL) or 56 zmol (9 fg) for IgG. It is expected that the device may be suitable for analysis with volumes down to tens of picoliters. 相似文献
43.
Jeroen Hoebeke Gerry Holderbeke Ingrid Moerman Bart Dhoedt Piet Demeester 《Wireless Personal Communications》2006,38(1):125-141
The fact that a lot of applications require secure communication to take place only between a dynamic subset of distributed devices sharing a common context, is, from a network point of view, very challenging and demanding. Existing technologies such as VPN, P2P overlays or VLANs can only partially respond to these requirements. This observation is the key factor that has driven the proposal of the virtual private ad hoc network concept. Virtual private ad hoc networks (VPAN) are secure and self-organizing overlay networks on top of existing IP infrastructure that use ad hoc networking techniques to enable network connectivity. The underlying IP infrastructure can be the Internet, cellular networks, ad hoc networks, mesh networks … or combinations thereof. A virtual private ad hoc overlay network creates a transparent, shielded and trusted environment for the applications and services running on the participants' devices. The overlay uses internal addressing and ad hoc routing, thereby forming a virtual network on top of the physical infrastructure. In addition, the overlay must be self-organizing and self-maintaining upon member mobility or membership changes. This paper gives an overview of the potential applications, a high-level network architecture and the network challenges emerging from the novel concept of virtual private ad hoc networking.
Jeroen Hoebeke was born in Ghent, Belgium in 1979. In 2002 he received the Masters degree in engineering (Computer Science) from the University of Ghent. In August 2002, he joined the Broadband Communications Networks Group. His PhD research includes the development of adaptive routing protocol techniques for mobile ad hoc networks. His main research interests are in ad hoc wireless communications and, more generally, in broadband wireless communications. Within the European MAGNET project, he is actively involved in the development of a network architecture and demonstrator for Personal Networks, with a prime focus on routing and connectivity.
Gerry Holderbeke was born in Zottegem, Belgium in 1982. He graduated in Informatics at the University of Ghent in 2004. In August 2004 he joined the Broadband Communications Networks Group where he is currently working as a project developer. His research currently includes the development of an emulator for mobile ad hoc networks. His main research interests are in ad hoc networks and broadband wireless communications and involve routing, addressing and more generally, communication within mobile ad hoc networks and infrastructured networks. Within the European MAGNET project, he is actively involved in the development of a network architecture for Personal Networks, with a prime focus on the implementation of the routing architecture.
Ingrid Moerman was born in Gent, Belgium in 1965. She received the degree in Electro-technical Engineering and the Ph.D degree from the Ghent University, Gent, Belgium in 1987 and 1992, respectively. Since 1987, she has been with the Interuniversity Micro-Electronics Centre (IMEC) at the Department of Information Technology (INTEC) of the Ghent University, where she conducted research in the field of optoelectronics. In 1997, she became a permanent member of the Research Staff at IMEC. Since 2000 she is part-time professor at the Ghent University. Since 2001 she has switched her research domain to broadband communication networks. She is currently involved in the research and education on broadband mobile & wireless communication networks and on multimedia over IP. The main research topics related to mobile & wireless communication networks are: wireless access to vehicles (high bandwidth & driving speed), adaptive QoS routing in wireless ad hoc networks, body area networks, protocol boosting on wireless links, design of fixed access/metro part, traffic engineering and QoS support in the wireless access network. Ingrid Moerman is author or co-author of more than 300 publications in the field of optoelectronics and communication networks.
Bart Dhoedt received a degree in Engineering from the Ghent University in 1990. In September 1990, he joined the Department of Information Technology of the Faculty of Applied Sciences, University of Ghent. His research, addressing the use of micro-optics to realize parallel free space optical interconnects, resulted in a PhD degree in 1995. After a 2 year post-doc in opto-electronics, he became professor at the Faculty of Applied Sciences, Department of Information Technology. Since then, he is responsible for several courses on algorithms, programming and software development. His research interests are software engineering and mobile & wireless communications. Bart Dhoedt is author or co-author of approximately 70 papers published in international journals or in the proceedings of international conferences. His current research addresses software technologies for communication networks, peer-to-peer networks, mobile networks and active networks.
Piet Demeester received the Masters degree in Electro-technical engineering and the Ph.D degree from the Ghent University, Gent, Belgium in 1984 and 1988, respectively. In 1992 he started a new research activity on broadband communication networks resulting in the IBCN-group (INTEC Broadband communications network research group). Since 1993 he became professor at the Ghent University where he is responsible for the research and education on communication networks. The research activities cover various communication networks (IP, ATM, SDH, WDM, access, active, mobile), including network planning, network and service management, telecom software, internetworking, network protocols for QoS support, etc. Piet Demeester is author of more than 300 publications in the area of network design, optimization and management. He is member of the editorial board of several international journals and has been member of several technical program committees (ECOC, OFC, DRCN, ICCCN, IZS, &). 相似文献
44.
According to the Environmental Protection Agency (EPA) and other public health agencies in the United States, radon may be the leading cause (along with passive smoking) of lung cancer deaths among nonsmokers. Radon is estimated to be the second leading cause of lung cancer death in smokers behind smoking-related lung cancer. EPA estimates that 7,000 to 30,000 lung cancer deaths each year are due to radon exposure. (It is implied that radon-related lung cancer deaths can be prevented by reducing radon levels below EPA's guideline levels.) Current EPA radon policy is based on a strategy of education, the transfer of testing and remediation technologies to the public and private sectors, and recently proposed radon-resistant construction standards for new homes. This paper models the effectiveness of current, proposed, and alternative policies for reducing radon risks in U.S. residential construction. The results of our analysis suggest that EPA's projections of 2,200 “lives saved annually” as a result of its current action level of 4 pCi/l will not be achieved with its current policy in the near future. Overall, the response of radon-related mortality to most policy options is delayed and flat due in pan to the large number of houses with low radon levels and the long latency period between radon exposure and the development of cancer. The modeling results suggest that more aggressive smoking reduction programs may yield greater benefits in overall lung cancer mortality (but not reduced radon exposure) than most radon-related policies. 相似文献
45.
Ingrid Löuh Thomas Frey Helmut A. Schaeffer 《Journal of the American Ceramic Society》1981,64(12):168-C
Influences on the oxidation state of glassmelts can be studied by using an electrochemical oxygen sensor which allows in-situ measurements at melting temperatures. The action of fining agents such as arsenic and sodium sulfate was investigated. The behavior of arsenic (i.e. release and uptake of oxygen) and the decomposition of sodium sulfate with and without carbon additions can be seen electrochemically. 相似文献
46.
Selenium biotransformations in an insect ecosystem: effects of insects on phytoremediation 总被引:2,自引:0,他引:2
Vickerman DB Trumble JT George GN Pickering II Nichol H 《Environmental science & technology》2004,38(13):3581-3586
Phytoremediation of selenium-contaminated soils may be influenced by higher trophic levels including insects. We examined how selenium affects the behavior, survival, and development of the wasp parasitoid Cotesia marginiventris, parasitizing its natural host, the beet armyworm Spodoptera exigua, feeding on alfalfa, Medicago sativa, irrigated with water containing selenate. X-ray absorption spectroscopy was used to quantify the selenium chemical forms in each trophic level. Alfalfa partially transformed selenate to organoselenium. S. exigua contained only organoselenium, both directly absorbed from M. sativa and transformed from selenate. C. marginiventris cocoons collected shortly after larval emergence contained only organoselenium derived from the host. The surprising finding of trimethylselenonium-like species in adult parasitoids and the cocoons from which they emerged suggests that adults and pharates can detoxify excess selenium through methylation and volatilization. Adult parasitoids do not discriminate against selenium-containing alfalfa, even though alfalfa generates selenium volatiles. Parasitoids raised on selenium-fed larvae emerged later and pupae weighed less than their selenium-free counterparts. We conclude therefore that C. marginiventris can be used to control S. exigua damage to M. sativa being used to remove selenium from soils. Moreover, the presence of such insects may improve phytoremediation by increasing biotransformation of inorganic selenium and release of volatile selenium species. 相似文献
47.
Davidson W McGibbon GA White PW Yoakim C Hopkins JL Guse I Hambly DM Frego L Ogilvie WW Lavallée P Archambault J 《Analytical chemistry》2004,76(7):2095-2102
An indandione-containing class of inhibitors abrogates DNA replication of human papillomavirus (HPV) types 6 and 11 by binding reversibly to the transactivation domain (TAD) of the viral E2 protein and inhibiting its interaction with the viral E1 helicase. To locate the binding site of this class of protein-protein interaction inhibitors, a benzophenone derivative was used to generate an irreversibly labeled E2-TAD polypeptide. The single site of covalent modification of the E2-TAD was identified by proteolytic digestions using trypsin, LysC, and V8 proteases and characterization of the resulting peptides by LC-MS procedures. Through this methodology, the benzophenone attachment point was located at the terminal methyl of residue Met101. Evidence further pinpointed the site of photoaffinity attachment to the terminal carbon atom, which is significant in providing a definitive example of the ability to locate photoinduced cross-linking to a polypeptide with atomic resolution using solely mass spectrometric detection. The location of the inhibitor binding site vis-à-vis the Glu39 and Glu100 residues sensitive to mutation for HPV 11 E2-TAD is discussed in relation to the crystal structure of the E2-TAD from the related HPV type 16. 相似文献
48.
49.
An electrochemical enzyme-linked immobilized DNA-hybridization assay for the detection of Cryptosporidium parvum in water has been developed. The target molecule was a 121-nucleotide sequence from the C. parvum heat shock protein 70 (hsp70 mRNA from U71181 gene). This analyte offers the possibility of distinguishing dead from live oocysts. The assay involves covalent attachment of a primary DNA probe via its 5'-amine-terminus to self-assembled monolayers of mercaptoundecanoic acid to a gold surface. The primary DNA probe was used to capture the target (sequence 1039-1082 of U71181 gene for the mRNA), by hybridization to a 20-base complementary sequence on the target (at sequence 1063-1082). A secondary DNA probe labeled with alkaline phosphatase (AP) was then hybridized to base sequence 1039-1062 on the target. p-Aminophenol, which is enzymatically generated by the immobilized AP from p-aminophenyl phosphate (PAPP), is detected using electrochemistry. The peak current of cyclic voltammograms from a PAPP solution, in which gold-coated silicon wafer modified with the complete assembly of the assay components was incubated, is linear with concentration of the target (5-50 microg/mL, where P1 and P2-AP concentrations are 50 microg/mL). A detection limit of 2 microg/mL (or 146 nM) of the DNA target was obtained. Cross-reactivity tests showed high selectivity for heat-shocked C. parvum. No signal was obtained for either the synthetic DNA for hsp70 of Campylobacter lari, Escherichia coli, Giardia lamblia, Salmonella typhimurium, and Listeria monocytogenes or for the products of heat-shocked whole organisms of E. coli, G. lamblia, Staphylococcus aureus, and Cryptosporidium muris. 相似文献
50.
The Hydrogeochemistry of Arsenic in
the Clara Mine,Germany 总被引:1,自引:1,他引:0
Yinian?Zhu Broder?J.?MerkelEmail author Ingrid?Stober Kurt?Bucher 《Mine Water and the Environment》2003,22(3):110-117
Abstract.
The oxidative dissolution of primary arsenic-bearing
sulfide minerals in barite-fluorite veins is a potential source
of arsenic in the Clara Mine. Geological structures, especially
the mineral veins, provide potential pathways for the water. The
highest arsenic concentrations are found in ground water within
the eastern part of the mine. Arsenic and major ions are
positively correlated and provide evidence that arsenic is
likely derived locally from the water-vein/water-rock
interaction. Geochemical modeling with PHREEQC shows all the
arsenate mineral phases to be significantly undersaturated,
although secondary arsenate minerals are common in the oxidized
part of the deposit. The mine waters plot near the boundary of
Fe(OH)3 and
Fe2+ in the pH-Eh diagram for the
As-Fe-S-H2O-system. Arsenic occurs as the
5-valent species
and
. Statistical analysis
illustrates a strong association between As, Fe, pH, and
HCO3. Oxidation of the primary As-bearing
minerals, such as pyrite and arsenopyrite, and the subsequent
behavior of the oxyanion, arsenate, generally controls the
distribution and speciation of arsenic. The low concentrations
of dissolved As is due to co-precipitation and adsorption of
arsenate by Fe, Al, and Mn (oxy)hydroxides. 相似文献