Survival of Listeria monocytogenes in a simulated dynamic gastrointestinal model during storage of inoculated bologna and salami slices in vacuum packages

Listeria monocytogenes counts were determined during storage (82 days, 4 degrees C) in vacuum packages of inoculated bologna and salami slices and after exposure to a simulated dynamic model of the stomach and small intestine. Variables controlled in the model included gastric emptying and gastrointestinal fluid secretion rates, gradual gastric acidification, and intestinal pH maintenance. L. monocytogenes populations increased on bologna and decreased on salami, reaching 8.7 and 1.4 log CFU/g, respectively, on day 82. Inactivation rates (IR) during gastric exposure of bologna and salami ranged from 0.079 (day 14) to 0.158 (day 57) log CFU/g/min and from 0.013 (day 42) to 0.051 (day 1) log CFU/g/min, respectively. On corresponding days, gastric IR for cells on salami were lower than on bologna, suggesting potential protective effects of the former product. However, it is also possible that the low initial L. monocytogenes levels reached with storage of salami (< or = 2.5 log CFU/g after day 27) may have resulted in slower reductions than in the high levels on bologna. Gradual decline of gastric pH allowed survival in the gastric compartment during the initial stages, which resulted in a large fraction of the cells being delivered into the intestinal compartment. Intestinal IR ranged from 0.003 to 0.048 (bologna) and from 0.002 to 0.056 (salami) log CFU/g/min throughout storage. Although findings indicated potential effects of salami against gastric killing of L. monocytogenes, any effects of the food matrix per se on the gastrointestinal survival of the pathogen were overwhelmed by the high and low contamination levels reached on bologna and salami, respectively, during storage.     

Assessment of quality of cod roes and relationship between quality and maturity stage

Cod roe landings are subject to great variability regarding their quality and subsequent price for the food industry. A scheme was developed for the classification of cod roe into four quality grades (A, B, C and D) based on appearance and texture attributes as described by cod roe traders. Evaluation of the maturity stage of 80 commercial cod ovaries through the frequency distribution of oocyte diameter and the percentage presence of empty follicular sacks revealed that cod ovaries with commercial value are at stage 4 (ripe or vitellogenic), stage 5 (spawning) and stage 6 (spent) of development. The designated maturity stages were found to be closely related to the described quality grades. This relationship implied that vitellogenic ovaries (stages 4a, 4b and 4c) were of A quality, early spawning ovaries (stage 5a) were most often classed as B quality, advanced and late spawning ovaries (stage 5b and 5c) were of C quality, whilst spent ovaries (stage 6) were of the lowest quality grade (D). Several physicochemical characteristics recorded (eg pH, membrane features) were also found to be related to the quality and the maturity stage of the ovaries. There is a significant relationship between roe quality and moisture content, with roes of higher quality having a lower moisture content. © 1999 Society of Chemical Industry     

Inkjet printing of photocatalytically active TiO2 thin films from water based precursor solutions

In this work, aqueous chemical solution deposition route suited for inkjet printing is used for the synthesis of photocatalytically active TiO₂ coatings. Environmentally friendly precursor solutions with electromagnetic ink-jet printing, allows cheap and simple processing of TiO₂ films on glass. The hydrolysis reaction of water sensitive titanium alkoxide (Ti-alkoxide) precursor is controlled by adding complexing agents as citric acid and triethanolamine prior to water addition, and aqueous stable solutions are achieved. The pH of the solutions is brought to neutral to guarantee flexible processing, avoid damage to substrates and equipment. Solution parameters are adapted to obtain optimal gelation conditions and good jettability. The influence of processing parameters on the phase formation and surface morphology is studied by thermogravimetric analysis and differential thermal analysis (TGA/DTA), X-ray diffraction (XRD), scanning electron microscopy (SEM) and atomic force microscopy (AFM). The photocatalytic activity of the films is evaluated by the degradation of methyl orange.     

What are users’ intentions towards real money trading in massively multiplayer online games?

This study investigates user behaviour in massively multiplayer online games from the perspective of their intentions to engage in real money trading. Players who engage in real money trading purchase resources instead of spending time to acquire them in the game. This behaviour influences not just their own gaming experience, but those of other players as well as the operator??s revenues. We present an online survey which targets the players of World of Warcraft. Players?? relationships with real money trading are investigated using insights from behavioural economics. We propose a model which includes a set of behavioural determinants grounded in empirical research on online games. The study??s findings indicate that a player??s social status and the disinhibiting effects of online play are positive influences on players?? intentions to engage in real money trading, while perceived fairness, anticipated regret and uncertainty about the seller??s behaviour are negative influences. Interestingly, neither the perceived enjoyment nor the potential punishments influence intentions.     

Expression and stabilization of galactose oxidase in Escherichia coli by directed evolution

We have used directed evolution methods to express a fungalenzyme, galactose oxidase (GOase), in functional form in Escherichiacoli. The evolved enzymes retain the activity and substratespecificity of the native fungal oxidase, but are more thermostable,are expressed at a much higher level (up to 10.8 mg/l of purifiedGOase), and have reduced negative charge compared to wild type,all properties which are expected to facilitate applicationsand further evolution of the enzyme. Spectroscopic characterizationof the recombinant enzymes reveals a tyrosyl radical of comparablestability to the native GOase from Fusarium.     

Wind characteristics in the North and Baltic Seas from the QuikSCAT satellite

The QuikSCAT mission provided valuable daily information on global ocean wind speed and direction from July 1999 until November 2009 for various applications including numerical weather prediction, ocean and atmospheric modelling. One new and important application for wind vector satellite data is offshore wind energy, where accurate and frequent measurements are required for siting and operating modern wind farms. The greatest advantage of satellite observations rests in their extended spatial coverage. This paper presents analyses of the 10 year data set from QuikSCAT, for the overview of the wind characteristics observed in the North and Baltic Seas, where most of Europe's offshore wind farms operate and more will be constructed. Significant issues in data availability are identified, directly related to the flagging schemes. In situ observations from three locations in the North Sea are used for comparisons. Mean biases (in situ minus satellite) are close to zero for wind speed and ‐2.7° for wind direction with a standard deviation of 1.2 m s ^{? 1} and 15°, respectively. The impact of using QuikSCAT and in situ measurements extrapolated to 10 m for wind power density estimations is assessed, accounting for possible influences of rain‐contaminated retrievals, the sample size, the atmospheric stability effects and either fitting the Weibull distribution or obtaining the estimates from the time series of wind speed observations.Copyright © 2012 John Wiley & Sons, Ltd.     

Revealing Nanomechanical Domains and Their Transient Behavior in Mixed-Halide Perovskite Films

Halide perovskites are a versatile class of semiconductors employed for high performance emerging optoelectronic devices, including flexoelectric systems, yet the influence of their ionic nature on their mechanical behavior is still to be understood. Here, a combination of atomic-force, optical, and compositional X-ray microscopy techniques is employed to shed light on the mechanical properties of halide perovskite films at the nanoscale. Mechanical domains within and between morphological grains, enclosed by mechanical boundaries of higher Young's Modulus (YM) than the bulk parent material, are revealed. These mechanical boundaries are associated with the presence of bromide-rich clusters as visualized by nano-X-ray fluorescence mapping. Stiffer regions are specifically selectively modified upon light soaking the sample, resulting in an overall homogenization of the mechanical properties toward the bulk YM. This behavior is attributed to light-induced ion migration processes that homogenize the local chemical distribution, which is accompanied by photobrightening of the photoluminescence within the same region. This work highlights critical links between mechanical, chemical, and optoelectronic characteristics in this family of perovskites, and demonstrates the potential of combinational imaging studies to understand and design halide perovskite films for emerging applications such as photoflexoelectricity.