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111.
Allaoua Achouri Joyce Irene Boye Denis Belanger Tiphaine Chiron Varoujan A. Yaylayan Faustinus K. Yeboah 《Food research international (Ottawa, Ont.)》2010,43(5):1494-1504
This study describes an extraction process for the preparation of highly purified calcium precipitated glycinin (11S). Initial extraction of soy proteins using isoelectric precipitation at pH 6.8 followed by cryo-precipitation yielded 4.2% product (11S) recovery with 98% protein purity for the control extracted with NaOH. Addition of calcium chloride (CaCl2) doubled the extraction yield (9%) compared to the control and when two other salts were used (i.e., sodium (Na2SO4) and ammonium (NH4)2SO4 sulfate, average yields of 4.4% and 5.17%, respectively). Thermal and molecular stability under varying conditions (pH, salts, SDS as a protein structure perturbing agent), and effect of glycation on functional and structural properties were investigated. Size exclusion chromatography and electrophoresis confirmed the predominance of a major band with MW of ~342 kDa with 98.4% purity. Differential scanning calorimetry (DSC) yielded one endothermic transition peak at 95.5 °C. Denaturation temperatures were >100 °C for all salt concentrations studied. The pH had a dominant influence on the structural properties of glycinin. In the presence of NaCl and CaCl2 (0.2–1 M), the protein structure showed very little denaturation and no aggregation bands were observed even on heating to 95 °C. Lower SDS concentrations (0.5–1%) resulted in denaturation and aggregation, while at 2% SDS only denaturation was observed. Glycation did not alter the conformational structure of protein. Improvements in surface properties were observed with moderate degree of glycation (6–24 h). 相似文献
112.
Monica Lira-Cantu Amine ChafiqJeremy Faissat Irene Gonzalez-VallsYouhai Yu 《Solar Energy Materials & Solar Cells》2011,95(5):1362-1374
In this work, we study the effect of the transparent conducting oxide (TCO) and the polymer applied (MEH-PPV or P3HT) on the photovoltaic properties of TCO/TiO2/polymer/Ag bi-layer solar cells. The solar cells were analyzed under inert atmosphere conditions resembling an encapsulated or sealed device. We demonstrate that the substrate applied, ITO or FTO, modifies the crystalline structure of the TiO2: on an ITO substrate, TiO2 is present in its anatase phase, on an FTO, the rutile phase predominates. Devices fabricated on an FTO, where the rutile phase is present, show better stability under inert atmospheres than devices fabricated on an ITO, anatase phase. With respect to the polymer, devices based on MEH-PPV show higher Voc (as high as 1 V), while the application of P3HT results in lower Voc, but higher Jsc and longer device stability. These observations have been associated to (a), the crystalline structure of TiO2 and (b) to the form the polymer is bonded to the TiO2 surface. In-situ IPCE analyses of P3HT-based solar cells show a red shift on the peak corresponding to TiO2, which is not present on the MEH-PPV-based solar cells. The latter suggest that P3HT can be linked to the TiO2 though the S-end atom, which results in devices with lower Voc. All these observations are also valid for devices, where the bare TiO2 is replaced by an Nb-TiO2. The application of an Nb-TiO2 with rutile structure in these polymer/oxide solar cells is the reason for their higher stability under inert atmospheres. We conclude that the application of TiO2 in its rutile phase is beneficial for long-term stability devices. Moreover there is an interplay between low Voc and Jsc in devices applying P3HT, since power conversion efficiency can be partially canceled by their lower Voc in comparison with MEH-PPV. These findings are important for polymer/oxide solar cells, but also for organic solar cells, where a layer of semiconductor oxides are in direct contact with a polymer, like in an inverted or tandem organic solar cells. 相似文献
113.
Irene Buj-Corral Joan Vivancos-Calvet 《The International Journal of Advanced Manufacturing Technology》2013,68(9-12):2517-2523
In the present work, the mixing process of different components of abrasive stones of cubic boron nitride used for rough honing was studied. Stones are made by the sintering process of abrasive grains, a metallic bond, and a humectant that favors covering of each abrasive grain by the bond. Incorrect mixing of abrasive grains with the bond and humectant can result in stones with nonuniform abrasive grain distribution. As the abrasive stone wears out, grain distribution will vary and modify efficiency of the honing operation. Tin is the metal having the lowest melting point among metals in the bond. By means of a scanning electron microscope and energy-dispersive X-ray microanalysis, tin segregation was discarded, which could have led to abrasive grain segregation. Later, mixing tests of the different components of the stones were performed at different mixing times. Both homogeneity degree of the abrasive content of different samples in a mix and covering degree of abrasive grains by bond were determined through two new parameters introduced in the present study. It was noted that use of a chain to accelerate the mixing process excessively reduces covering degree of abrasive grains even at initial mixing times. The highest quantity of humectant is recommended in order to get better covering degree. Mixing time should be high enough to assure homogeneity degree of the mix but low enough to avoid excessive reduction of covering degree. 相似文献
114.
Nicolette Pegels Isabel González Irene Martín María Rojas Teresa García Rosario Martín 《Food Control》2011,22(8):1189-1196
A TaqMan real-time polymerase chain reaction (PCR) method was developed for specific detection of bovine, ovine and caprine processed animal protein (PAP) in industrial feedstuffs. The method uses species-specific primers and probes targeting short mitochondrial D-loop sequences, and a positive amplification control based on 18S rRNA gene. The applicability of the real-time PCR protocol was assessed through analysis of 126 industrial feed samples that were manufactured to reproduce rendering processes of commercial feeds destined for farmed animals. The assay successfully classified samples as positive or negative according to the ruminant composition, enabling qualitative detection of banned material in feeds at levels as low as 0.1%. Although the method provides quantitative potential, results suggest that the real quantitative capability of the assay is limited by the existing variability in terms of composition and processing treatments of the feeds, which affect the amount and quality of amplifiable DNA. 相似文献
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117.
Walden Brent; McGue Matt; lacono William G.; Burt S. Alexandra; Elkins Irene 《Canadian Metallurgical Quarterly》2004,113(3):440
Although behavior genetic studies have suggested that early substance use is primarily environmentally mediated, no study has sought to identify the specific sources of environmental variance. Using data obtained from multiple informants, this study assessed the contributions of peer deviance and parent-child relationship problems to substance use in 14-year-old male and female twins (N = 1,403) drawn from the Minnesota Twin Family Study (MTFS). All three phenotypes were influenced primarily by shared environmental variance (average c2 = .51), as was the overlap among them. Moreover, peer deviance and parent-child relationship problems accounted for approximately 77% of the variance in early substance use. Findings also indicated that peer deviance, but not parent-child relationship problems, accounted uniquely for variance in early substance use. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
118.
Familial Dysautonomia (FD) is an autosomal recessive disease of unknown etiology, occurring primarily in Ashkenazi Jews. Patients
are neurologically impaired, with deficits primarily in autonomic and sensory functions. The biochemical and genetic defects
have remained elusive, precluding carrier detection and prenatal diagnosis. High-performance liquid chromatography data indicated
up to a threefold increase in the neutral glycosphingolipid globotriaosylceramide in Dysautonomic fibroblasts and lymphoblasts.
Total ganglioside values, measured by colorimetric, fluorometric or specific sodium borohydride incorporation, were decreased.
Affected fibroblasts exhibited a range of pleomorphic phenotypes, such that the usual swirl-like confluent growth pattern
of normal fibroblasts was distorted to varying degrees, suggesting abnormalities in the FD plasma membrane, possibly affecting
cell-cell contacts. The glycosphingolipid increase could not be accounted for on the basis of markedly decreased α-galactosidase
activity, as in Fabry's disease, where patients also display decreased autonomic function. 相似文献
119.
Kim Irene J.; Ge Xiaojia; Brody Gene H.; Conger Rand D.; Gibbons Frederick X.; Simons Ronald L. 《Canadian Metallurgical Quarterly》2003,17(4):571
The occurrence and co-occurrence of depressive symptoms and conduct problems were examined in the context of parenting behaviors in a community-based sample of 897 African American children and their primary caregivers using a multi-informant, longitudinal design. Parenting behaviors and clinical symptoms were assessed in 2 waves, when the children averaged 10.5 and 12.3 years of age. Parenting behaviors differed significantly according to a child's symptomatology; (a) that is, when a child exhibited no depressive or conduct problems, (b) depressive problems only, (c) conduct problems only, or (d) co-occurring depressive and conduct problems. When parenting behaviors were examined according to changes in children's symptom levels, children whose symptoms increased over time reported increases in hostility and harsh-inconsistent parenting and decreases in warmth and nurturant-involved parenting. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
120.
José A. Carrillo Irene M. Gamba Armando Majorana Chi-Wang Shu 《Journal of Computational Electronics》2003,2(2-4):375-380
We present preliminary results of a high order WENO scheme applied to deterministic computations for two dimensional formulation of the transients for the Boltzmann-Poisson system describing electron transport in semiconductor devices. The collisional term models optical-phonon interactions which become dominant under strong energetic conditions corresponding to nanoscale active regions under applied bias. We treat the Boltzmann Transport equation in a spherical coordinate system for the wave-vector space. The problem is three dimensional in the wave-vector space and two dimensional in the physical space, plus the time variable driving to steady states. The new formulation avoids the singularity due to the spherical coordinate system. 相似文献