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61.
Chemical characterization of 21 species of marine macroalgae common in Norwegian waters: benefits of and limitations to their potential use in food and feed
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Irene Biancarosa Ikram Belghit Christian G Bruckner Nina S Liland Rune Waagbø Heidi Amlund Erik‐Jan Lock 《Journal of the science of food and agriculture》2018,98(5):2035-2042
BACKGROUND
In the past few years, much effort has been invested into developing a new blue economy based on harvesting, cultivating and processing marine macroalgae in Norway. Macroalgae have high potential for a wide range of applications, e.g. as source of pharmaceuticals, production of biofuels or as food and feed. However, data on the chemical composition of macroalgae from Norwegian waters are scant. This study was designed to characterize the chemical composition of 21 algal species. Both macro‐ and micronutrients were analysed. Concentrations of heavy metals and the metalloid arsenic in the algae were also quantified.RESULTS
The results confirm that marine macroalgae contain nutrients which are relevant for both human and animal nutrition, the concentrations whereof are highly dependent on species. Although heavy metals and arsenic were detected in the algae studied, concentrations were mostly below maximum allowed levels set by food and feed legislation in the EU.CONCLUSION
This study provides chemical data on a wide range of algal species covering the three taxonomic groups (brown, red and green algae) and discusses both benefits of and potential limitations to their use for food and feed purposes. © 2017 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. 相似文献62.
Antonio Raffo Irene Baiamonte Nicoletta Nardo Flavio Paoletti 《European Food Research and Technology》2007,225(3-4):395-405
Physical (weight, firmness) and compositional (sugars, organic acids, ascorbic acid, phenolic compounds and carotenoids) changes
of red sweet peppers (Capsicum annuum L.) were monitored during 21 days of cold storage (at 7.5 °C); fruits were stored without packaging, packaged in low density
polyethylene bags, or after hot water dipping (53 °C for 4 min) and packaging. Packaging prevented water loss, and preserved
the firmness of the fresh product. Sugars (fructose and glucose) content was practically constant throughout the whole storage
time, for all treatments. A moderate accumulation of citric acid was observed during storage, but no marked effects of packaging
and hot water dipping on citric and malic acid content. Ascorbic acid content slightly increased in unpackaged and packaged
fruits, but not in treated+packaged peppers. Hydroxycinnamics total content seemed not to be affected by cold storage, packaging
or hot water treatment, whereas glycosylated flavonoids showed somewhat lowered levels during storage, particularly in the
case of unpackaged and packaged+treated fruits. Regarding carotenoids content, the effect of the considered storage conditions
seemed to be much smaller than that due to ripening stage. Provitamin A content showed an increasing trend in unpackaged and
packaged fruits; packaged+treated peppers were characterised by a lower retention of provitamin A and a higher level of capsanthin
and cucurbitaxanthin A with respect to not treated fruits. On the whole, packaging and hot water treatment did not produce
noticeable adverse effects on the majority of the examined compositional quality parameters. 相似文献
63.
Unai Iriarte‐Velasco Irene Sierra Emilio Atilano Cepeda Raquel Bravo Jose Luis Ayastuy 《Coloration Technology》2015,131(4):322-332
Bone is an inorganic template containing organic material inside which can be converted into hydroxyapatite‐rich material by pyrolysis. Nowadays, there is a growing research interest in the use of hydroxyapatite, the chemical formula of which is Ca10(PO4)6(OH)2. In the present work, pork bone, an abundant biomass source and food waste, has been converted into structured porous hydroxyapatite by a three‐step process including precharring under mild conditions, chemical activation, and thermal activation. The investigated activating agents were NaOH, KOH, K2CO3, H2SO4, and H3PO4. A thorough investigation of the influence of different activating protocols on the chemical and textural properties of the produced material was carried out by nitrogen adsorption–desorption at 77 K, potentiometric titrations, Fourier transform infrared, and X‐ray diffraction techniques. Chemical activation with NaOH, K2CO3, and H2SO4 increased the specific surface area up to 53%. H3PO4 reduced both surface area and pore volume, and KOH showed little influence on the pore structure. The produced materials were evaluated by methylene blue adsorption tests and showed significant improvement as a result of chemical activation. As a main effect, acid treatment increased methylene blue adsorption kinetics, probably owing to an increase in micropororosity, whereas alkali activation enhanced the adsorption capacity of the resultant biochar. 相似文献
64.
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66.
Diana Valeria Rossetti Ilaria Inserra Alessia Nestic Federica Vincenzoni Federica Iavarone Irene Messana Massimo Castagnola Luca Massimi Gianpiero Tamburrini Massimo Caldarelli Claudia Desiderio 《International journal of molecular sciences》2022,23(6)
The present investigation aimed to explore the intact proteome of tissues of pediatric brain tumors of different WHO grades and localizations, including medulloblastoma, pilocytic astrocytoma, and glioblastoma, in comparison with the available data on ependymoma, to contribute to the understanding of the molecular mechanisms underlying the onset and progression of these pathologies. Tissues have been homogenized in acidic water–acetonitrile solutions containing proteases inhibitors and analyzed by LC–high resolution MS for proteomic characterization and label-free relative quantitation. Tandem MS spectra have been analyzed by either manual inspection or software elaboration, followed by experimental/theoretical MS fragmentation data comparison by bioinformatic tools. Statistically significant differences in protein/peptide levels between the different tumor histotypes have been evaluated by ANOVA test and Tukey’s post-hoc test, considering a p-value > 0.05 as significant. Together with intact protein and peptide chains, in the range of molecular mass of 1.3–22.8 kDa, several naturally occurring fragments from major proteins, peptides, and proteoforms have been also identified, some exhibiting proper biological activities. Protein and peptide sequencing allowed for the identification of different post-translational modifications, with acetylations, oxidations, citrullinations, deamidations, and C-terminal truncations being the most frequently characterized. C-terminal truncations, lacking from two to four amino acid residues, particularly characterizing the β-thymosin peptides and ubiquitin, showed a different modulation in the diverse tumors studied. With respect to the other tumors, medulloblastoma, the most frequent malignant brain tumor of the pediatric age, was characterized by higher levels of thymosin β4 and β10 peptides, the latter and its des-IS form particularly marking this histotype. The distribution pattern of the C-terminal truncated forms was also different in glioblastoma, particularly underlying gender differences, according to the definition of male and female glioblastoma as biologically distinct diseases. Glioblastoma was also distinguished for the peculiar identification of the truncated form of the α-hemoglobin chain, lacking the C-terminal arginine, and exhibiting oxygen-binding and vasoconstrictive properties different from the intact form. The proteomic characterization of the undigested proteome, following the top-down approach, was challenging to originally investigate the post-translational events that differently characterize pediatric brain tumors. This study provides a contribution to elucidate the molecular profiles of the solid tumors most frequently affecting the pediatric age, and which are characterized by different grades of aggressiveness and localization. 相似文献
67.
Development and characterization of bionanocomposites based on poly(3‐hydroxybutyrate) and cellulose nanocrystals for packaging applications
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Irene T Seoane Elena Fortunati Debora Puglia Viviana P Cyras Liliana B Manfredi 《Polymer International》2016,65(9):1046-1053
Poly(3‐hydroxybutyrate) (PHB)‐based bionanocomposites were prepared using various percentages of cellulose nanocrystals (CNCs) by a solution casting method. CNCs were prepared from microcrystalline cellulose using sulfuric acid hydrolysis. The influence of CNCs on PHB properties was evaluated using differential scanning calorimetry, Fourier transform infrared spectroscopy, X‐ray diffraction, thermogravimetry and tensile testing. Vapor permeation and light transmission of the materials were also measured. Differential scanning calorimetric tests demonstrated that CNCs were effective PHB nucleation agents. Tensile strength and Young's modulus of PHB increased with increasing CNC concentration. Moreover, the PHB/CNC bionanocomposites exhibited reduced water vapor permeation compared to neat PHB and had better UV barrier properties than commodity polymers such as polypropylene. It was found that nanocomposites with 6 wt% of CNCs had the optimum balance among thermal, mechanical and barrier properties. © 2016 Society of Chemical Industry 相似文献
68.
Wolter Marc Tedjo-Palczynski Irene Hentschel Bernd Kuhlen Torsten 《Computer Graphics and Applications, IEEE》2009,29(6):54-64
Scientific-visualization tools can make time-varying simulations easier to understand. The growing efficiency of today's high-performance computers enables simulation of physical phenomena with a high temporal resolution. Consequently, visualization systems require efficient navigation in the temporal dimension. This 3D user interface employs direct-manipulation metaphors for temporal navigation in scientific visualizations. By interacting with objects using their 3D trajectory, users can navigate in time by specifying spatial inputs. This article is part of a special issue on 3D user interfaces. 相似文献
69.
Dr. Irene Amata Mariano Maffei Dr. Ana Igea Dr. Marina Gay Dr. Marta Vilaseca Dr. Angel R. Nebreda Prof. Dr. Miquel Pons 《Chembiochem : a European journal of chemical biology》2013,14(14):1820-1827
Intrinsically disordered regions (IDRs) are preferred sites for post‐translational modifications essential for regulating protein function. The enhanced local mobility of IDRs facilitates their observation by NMR spectroscopy in vivo. Phosphorylation events can occur at multiple sites and respond dynamically to changes in kinase–phosphatase networks. Here we used real‐time NMR spectroscopy to study the effect of kinases and phosphatases present in Xenopus oocytes and egg extracts on the phosphorylation state of the “unique domain” of c‐Src. We followed the phosphorylation of S17 in oocytes, and of S17, S69, and S75 in egg extracts by NMR spectroscopy, MS, and western blotting. Addition of specific kinase inhibitors showed that S75 and S69 are phosphorylated by CDKs (cyclin‐dependent kinases) differently from Cdk1. Moreover, although PKA (cAMP‐dependent protein kinase) can phosphorylate S17 in vitro, this was not the major S17 kinase in egg extracts. Changes in PKA activity affected the phosphorylation levels of CDK‐dependent sites, thus suggesting indirect effects of kinase–phosphatase networks. This study provides a proof‐of‐concept of the use of real‐time in vivo NMR spectroscopy to characterize kinase/phosphatase effects on intrinsically disordered regulatory domains. 相似文献
70.
Tapé is a popular delicacy in Indonesia that is prepared by fermenting starch-rich material. Yeast plays an important role in the making of tapé especially in the aroma and taste of the product. In this study, yeasts were isolated from tapé and subjected to diversity analysis using the restriction fragment length polymorphism (RFLP) technique on 5.8S rRNA encoding gene and the internal transcribed spacer (ITS). A total of 21 yeast isolates were obtained from 3 different types of Indonesian tapé (cassava tapé, white-, and black-glutinous rice tapé). The identified yeast species from cassava tapé were Pichia jadinii, Candida glabrata, and Clavispora lusitaniae. P. fabianii, Rhodotorula mucilaginosa, and Ogataea polymorpha were obtained from white glutinous rice tapé, while Saccharomycopsis fibuligera and Wickerhamomyces anomalus were obtained from black glutinous rice tapé. 相似文献