Effects of low- or medium-pressure ultraviolet lamp irradiation on Microcystis aeruginosa and Anabaena variabilis

A pure culture of Microcystis aeruginosa or Anabaena variabilis, the representatives of water blooming algae, was exposed to low-pressure (LP) or medium-pressure (MP) UV lamps. Irradiated pure culture suspension was subsequently incubated for 7d under white light fluorescent lamps. During incubation, profiles of the number of cells, DNA damage and photosynthetic activity were determined. When UV fluence was 600mJ/cm(2), M. aeruginosa cell numbers decreased throughout the 7-d incubation period, to produce 1.5log reduction (LP) or 1.2log reduction (MP) compared with control. The amount of DNA damage was 2.02x10(-4) ESS/base (LP) and 3.42x10(-4) ESS/base (MP) just after UV irradiation, which became 0.05x10(-4) ESS/base and 0.23x10(-4) ESS/base, respectively, after 3d incubation. However, cell number kept decreasing, even after DNA repair. Photosynthetic activity decreased by 1.5log within 1d (LP) or 3d (MP). Thus, reduction in photosynthetic activity could contribute to the reduction in M. aeruginosa cell numbers. A. variabilis cell numbers reduced by 2.3log (LP) or 2.2log (MP) during the 7-d incubation period; however, after DNA damage repair, cell number began to increase. The amount of DNA damage was 6.07x10(-4) ESS/base (LP) and 4.48x10(-4) ESS/base (MP) just after UV irradiation, which became 0.23x10(-4) ESS/base and 0.40x10(-4) ESS/base, respectively, after 3d incubation. No reduction was observed in photosynthetic activity/cell. Therefore, DNA damage is the main contributor of the reduction in cell number of A. variabilis.     

Application of microalgae hydrolysate as a fermentation medium for microbial production of 2-pyrone 4,6-dicarboxylic acid

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Microstructure and Softening of Laser-Welded 960 MPa Grade High Strength Steel Joints

The microstructural evolution of laser-welded 960 MPa grade high strength steel joints and its effect on softening behavior of heat affected zone (HAZ) were investigated in this paper. The results show that microstructure of HAZ and fusion zone (FZ) is composed of lath martensite and bainitic ferrite. The microstructure of mixed grained zone presents strip-like characteristics and small block martensite distributes along the grain boundary. The grain size near the fusion line is about 25 μm, and the grain size in the fine grain zone is less than 5 μm. Microhardness of HAZ and FZ is lower than base metal. The soft zone locates in transitional region between tempering zone and mixed grained zone due to the interaction of the martensite tempering and the recovery and recrystallization of the rolled microstructure. Microhardness of soft zone is 310 HV and drops 18% compared to base material. Welding heat input has a remarkable effect on the width of soft zone and microhardness. The tensile properties of weld joints are closely related to the softening behavior of HAZ.     

Review of laser welding monitoring

Laser welding, as a highly efficient processing technology, has been widely applied to manufacturing industry. This paper makes an overview on real time monitoring of laser welding. It begins with a detailed introduction to six typical sensors (photodiode, visual, spectrometer, acoustical sensor, pyrometer, plasma charge sensor) in laser welding detection. Then it makes a review on multi-sensor fusion technology in both laser welding monitoring and adaptive control. Last, subjects for future research concerning welding monitoring and control have been proposed. The paper concludes that the real-time monitoring of laser welding can provide a great amount of valid information about welding status to help effectively identify weld defects and realize adaptive control.     

Three-scale analysis of BaTiO<Subscript>3</Subscript> piezoelectric thin films fabrication process and its experimental validation

A three-scale analysis of crystal growth process is newly proposed based on the first-principles calculation and on the finite element analysis in order to generate a new biocompatible piezoelectric thin film. Crystal growth process of lead-free BaTiO₃ thin films was designed and experimentally generated on SrTiO₃(100), (110), (111), and MgO(100) substrates using the radio-frequency magnetron sputtering method. Crystal structures of BaTiO₃ were measured by X-ray diffraction (XRD) θ/2θ scan. We used Pt for the electrode and measured piezoelectric strain constants d ₃₃ using the ferroelectric measurement system. As a result, analytical crystal orientation fractions on SrTiO₃(110) and (111) substrates had good quantitative agreement with experimental ones, and ones on SrTiO₃(100) and MgO(100) substrates corresponded with these experimental crystal structures. Furthermore, analytically determined piezoelectric strain constants d ₃₃ qualitatively showed a good agreement with experimental ones. Especially, for SrTiO₃(100) and MgO(100) substrates, the differences of d ₃₃ depending on orientation fractions were analyzed by the three-scale simulation accurately. Consequently, it is confirmed that the three-scale analysis is a useful simulation tool to design new biocompatible piezoelectric thin films.     

Fundamental welding R&D results for manufacturing vacuum vessel of JT-60SA

The real vacuum vessel (VV) manufacturing of JT-60SA has started since November 2009 at Toshiba. Prior to starting manufacturing, fundamental welding R&Ds had been performed by three stages. In the first stage, primary tests for screening welding method were performed. In the second stage, the trial welding for 1 m-long straight and curved double shell samples were conducted. The dependences of welding quality and distortion on the welding conditions, such as arc voltage and current, setting accuracy, welding sequence, and the shape of grooves were studied. In addition, welding condition with low heat input was explored. In the last stage, fabrication sequence was confirmed and established by the trial manufacturing of the 20° upper half mock-up [1]. This paper presents the R&D results obtained in the first and second stages.     

Hydrogen incorporation in tungsten deposits growing by deuterium plasma sputtering

Tungsten deposits were produced by sputtering method using hydrogen isotope RF plasma, and the density and the incorporated components in the deposits were investigated. The density changed in the range from 14.2 g/cm³ to 6.1 g/cm³, and hydrogen isotope retention changed in the range from 0.25 to 0.05 as (H + D)/W by the difference of deposition conditions. Both the density and hydrogen isotope retention tended to decrease with an increase of pressure. Even though a deuterium gas was used for producing tungsten deposits, not only deuterium but also hydrogen, oxygen and water vapor were incorporated in the deposits. It is considered that the incorporation of these components originated in water vapor unintentionally existing in the vacuum chamber.     

Effect of DNA topoisomerase I inhibitor on the transduction efficiency of an deno-associated virus vector in human airway epithelial cells

We tested the effects of a DNA topoisomerase inhibitor (camptothecin; CPT) on the transduction efficiency of AAV vectors in cultured human airway epithelial cells. The cells were treated with CPT for 24 hours, then exposed to AAV-CMV-LacZ for 1 hour at different multiplicities of infection (moi). Transduction efficiency of AAV vectors was assessed using X-gal staining as the percentage of LacZ-expressing cells. The transduction efficiency was approximately 1.5 to 10 fold increased by treatment with CPT prior to AAV vector exposure. However, treatment with CPT after AAV vector infection did not enhance the transduction efficiency of the vectors. These results suggest that pre-treatment with CPT increases the transduction efficiency of AAV vectors, probably by nodulating cellular function.