全文获取类型
收费全文 | 112篇 |
免费 | 1篇 |
专业分类
化学工业 | 22篇 |
金属工艺 | 1篇 |
机械仪表 | 2篇 |
建筑科学 | 7篇 |
轻工业 | 15篇 |
无线电 | 7篇 |
一般工业技术 | 11篇 |
冶金工业 | 31篇 |
自动化技术 | 17篇 |
出版年
2024年 | 1篇 |
2023年 | 1篇 |
2022年 | 2篇 |
2021年 | 4篇 |
2020年 | 3篇 |
2019年 | 1篇 |
2018年 | 2篇 |
2017年 | 3篇 |
2014年 | 1篇 |
2013年 | 3篇 |
2012年 | 8篇 |
2011年 | 8篇 |
2010年 | 7篇 |
2009年 | 1篇 |
2008年 | 5篇 |
2007年 | 2篇 |
2006年 | 7篇 |
2005年 | 6篇 |
2004年 | 5篇 |
2003年 | 7篇 |
2002年 | 1篇 |
2001年 | 8篇 |
2000年 | 2篇 |
1998年 | 5篇 |
1997年 | 6篇 |
1996年 | 3篇 |
1995年 | 1篇 |
1994年 | 2篇 |
1993年 | 2篇 |
1992年 | 3篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1982年 | 1篇 |
排序方式: 共有113条查询结果,搜索用时 281 毫秒
31.
Kivim?ki Mika; Vahtera Jussi; Elovainio Marko; Helenius Hans; Singh-Manoux Archana; Pentti Jaana 《Canadian Metallurgical Quarterly》2005,24(4):413
The authors prospectively examined changes in health after a major life event (death or onset of severe illness in family) among 5,007 employees (mean age=44.8 years) whose optimism and pessimism levels were assessed in 1997 and major life events in 2000. Health was indicated by sickness absence days during a period covering 36 months prior to the event and 18 months after the event. Increase in sick days after the event was smaller and returned to the preevent level more quickly among highly optimistic individuals than among their counterparts with low optimism. Parallel changes were not observed in relation to pessimism. These findings suggest that optimism may reduce the risk of health problems and may be related to a faster recovery after a major life event. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
32.
New labels and assay techniques are needed to improve the sensitivity and quantitativeness of point-of-care immunotesting while sustaining the rapidity and ease of use of the assays. We synthesized a novel, intrinsically fluorescent nonadentate europium chelate with two chromophores and hydrophilic alpha-galactose side groups. The chelate is highly fluorescent, soluble in water, and provides effective shielding of Eu from water. The performance of the nonadentate chelate was compared with a heptadentate chelate in a dry reagent immunoassay for human chorionic gonadotropin (hCG). After 15-min incubation and washing, time-resolved fluorescence was measured directly from a wet or dried well surface. Contrary to the heptadentate label, the effect of aqueous quenching on the nonadentate label was found to be insignificant, with calculated analytical detection limits (background + 3 SD) of 0.9 and 0.7 IU/L hCG for wet and dry measurements, respectively, and a linear range up to 5000 IU/L. The CVs for the new label were <8% at the cutoff of 25 IU/L and above in both whole blood and plasma. The novel nonadentate label facilitates short turnaround times and simple instrumentation due to the absence of all signal development steps, at the same time retaining an excellent immunoassay performance. 相似文献
33.
Tarvainen MP Hiltunen JK Ranta-aho PO Karjalainen PA 《IEEE transactions on bio-medical engineering》2004,51(3):516-524
An adaptive spectrum estimation method for nonstationary electroencephalogram by means of time-varying autoregressive moving average modeling is presented. The time-varying parameter estimation problem is solved by Kalman filtering along with a fixed-interval smoothing procedure. Kalman filter is an optimal filter in the mean square sense and it is a generalization of other adaptive filters such as recursive least squares or least mean square. Furthermore, by using the smoother the unavoidable tracking lag of adaptive filters can be avoided. Due to the properties of Kalman filter and benefits of the smoothing the time-frequency resolution of the presented Kalman smoother spectra is extremely high. The presented approach is applied to estimation of event-related synchronization/desynchronization (ERS/ERD) dynamics of occipital alpha rhythm measured from three healthy subjects. With the Kalman smoother approach detailed spectral information can be extracted from single ERS/ERD samples. 相似文献
34.
Jaana Schneider Marianne Pultar Johannes Oesterreicher Madhusudhan Reddy Bobbili Severin Mühleder Eleni Priglinger Heinz Redl Andreas Spittler Johannes Grillari Wolfgang Holnthoner 《International journal of molecular sciences》2021,22(8)
Coculture systems employing adipose tissue-derived mesenchymal stromal/stem cells (ASC) and endothelial cells (EC) represent a widely used technique to model vascularization. Within this system, cell–cell communication is crucial for the achievement of functional vascular network formation. Extracellular vesicles (EVs) have recently emerged as key players in cell communication by transferring bioactive molecules between cells. In this study we aimed to address the role of EVs in ASC/EC cocultures by discriminating between cells, which have received functional EV cargo from cells that have not. Therefore, we employed the Cre-loxP system, which is based on donor cells expressing the Cre recombinase, whose mRNA was previously shown to be packaged into EVs and reporter cells containing a construct of floxed dsRed upstream of the eGFP coding sequence. The evaluation of Cre induced color switch in the reporter system via EVs indicated that there is no EV-mediated RNA transmission either between EC themselves or EC and ASC. However, since Cre mRNA was not found present in EVs, it remains unclear if Cre mRNA is generally not packaged into EVs or if EVs are not taken up by the utilized cell types. Our data indicate that this technique may not be applicable to evaluate EV-mediated cell-to-cell communication in an in vitro setting using EC and ASC. Further investigations will require a functional system showing efficient and specific loading of Cre mRNA or protein into EVs. 相似文献
35.
D. Fantozzi V. Matikainen M. Uusitalo H. Koivuluoto P. Vuoristo 《Journal of Thermal Spray Technology》2018,27(1-2):220-231
Highly corrosion- and wear-resistant thermally sprayed chromium carbide (Cr3C2)-based cermet coatings are nowadays a potential highly durable solution to allow traditional fluidized bed combustors (FBC) to be operated with ecological waste and biomass fuels. However, the heat input of thermal spray causes carbide dissolution in the metal binder. This results in the formation of carbon saturated metastable phases, which can affect the behavior of the materials during exposure. This study analyses the effect of carbide dissolution in the metal matrix of Cr3C2-50NiCrMoNb coatings and its effect on chlorine-induced high-temperature corrosion. Four coatings were thermally sprayed with HVAF and HVOF techniques in order to obtain microstructures with increasing amount of carbide dissolution in the metal matrix. The coatings were heat-treated in an inert argon atmosphere to induce secondary carbide precipitation. As-sprayed and heat-treated self-standing coatings were covered with KCl, and their corrosion resistance was investigated with thermogravimetric analysis (TGA) and ordinary high-temperature corrosion test at 550 °C for 4 and 72 h, respectively. High carbon dissolution in the metal matrix appeared to be detrimental against chlorine-induced high-temperature corrosion. The microstructural changes induced by the heat treatment hindered the corrosion onset in the coatings. 相似文献
36.
Jaana Pinnick Andrew Riddick Robert McLaverty Garry Baker 《New Review of Information Networking》2018,23(1-2):99-110
The British Geological Survey (BGS) is responsible for managing a major geoscience data archive on behalf of the UK Nuclear Decommissioning Authority (NDA). Much of this geological data was captured during the 1990s and early 2000s using now obsolete software and data formats. This data asset remains an important resource for the NDA and the wider scientific community. The NDA wishes to ensure the data remain accessible and usable for many decades into the future. BGS has been working closely with Radioactive Waste Management (RWM), a wholly owned subsidiary of the NDA, on a program of data management and digital continuity measures to ensure the long-term usability of the data. This article describes some of the challenges and outlines the approaches we have taken to address these issues. 相似文献
37.
38.
The SEB1/SBH1 and the SSO genes encode components of the protein secretory machinery functioning at the opposite ends, ER translocation and exocytosis, respectively, of the secretory pathway in Saccharomyces cerevisiae. Overexpression of these genes can rescue temperature-sensitive (ts) growth defect of many sec mutants impaired in protein secretion. Furthermore, their overexpression in wild-type yeast enhances production of secreted proteins in S. cerevisiae, which suggests that they may be rate-limiting factors in this process. Here we report isolation of Kluyveromyces lactis homologues of these genes. KlSSO1 and KlSEB1 were isolated as clones capable of rescuing growth of ts sso2-1 and seb1Delta seb2Delta sem1Delta strains, respectively, at the restrictive temperature. The encoded Kluyveromyces proteins are up to 70% identical with the S. cerevisiae homologues at the amino acid level and can functionally replace them. Interestingly, KlSSO1 and KlSEB1 show similar enhancing effect on production of a secreted protein as the SSO and SEB1 genes of S. cerevisiae when overexpressed. In accordance with the high homology level of the secretory pathway proteins in different yeast species, the polyclonal antibodies raised against S. cerevisiae Seb1p, Sso2p and Sec4p can detect homologous proteins in cell lysates of K. lactis and Pichia pastoris, the latter also in Candida utilis. The GenBank Accession Nos are AF307983 (K. lactis SSO1) and AF318314 (K. lactis SEB1). 相似文献
39.
T Ikonen M Uusitalo E Taskinen A Korpela US Salminen RE Morris AL Harjula 《Canadian Metallurgical Quarterly》1998,17(10):945-953
The subcellular localization of the phosphatidylglycerol/phosphatidylinositol transfer protein (PG/PI-TP) of Aspergillus oryzae was investigated using Western blot analysis of the cell protein extracts, a cellular membrane fractionation technique, and transmission electron microscopy. The PG/PI-TP, as detected by Western blot analysis with a specific immune serum, was found to be mainly cytoplasmic and partly associated with intracellular membranes. A fractionation experiment was conducted after homogenization of the filamentous fungus mycelium. The endoplasmic reticulum, Golgi-like vesicles, and the plasma membrane were separated by isopycnic ultracentrifugation on a sucrose gradient, and our data revealed that the immunodetected PG/PI-TP was only associated with the Golgi-like apparatus. All these results were documented by electron microscopy and indicate here for the first time that there exists a specific phospholipid transfer protein in a filamentous fungus that is localized in the cytoplasm and associated with Golgi-like vesicles. 相似文献
40.
T Ikonen AR Sovij?rvi E Taskinen M Uusitalo L Kivisaari P Aarnio AL Harjula 《Canadian Metallurgical Quarterly》1997,29(1):12-19
153 regional lymph nodes obtained during surgical operations because of cancer of various cites from 50 cancer patients were studied. Failure of local immunity is explained by the following factors: 1) depletion of T-cell population with a decrease of activated lymphocyte-killers; 2) high activity of B-cell population able to block T-cell cytotoxic effect; 3) deficiency of T-suppressors and T-helpers; 4) a decrease of macrophages-monocytes of phagocytic capacity; 5) high activity of dendritic reticular cells stimulating transformation of B-lymphocytes and low activity of interdigitating reticulocytes determining T-cell immunity stimulation. 相似文献