Can Developments in Tissue Optical Clearing Aid Super-Resolution Microscopy Imaging?

The rapid development of super-resolution microscopy (SRM) techniques opens new avenues to examine cell and tissue details at a nanometer scale. Due to compatibility with specific labelling approaches, in vivo imaging and the relative ease of sample preparation, SRM appears to be a valuable alternative to laborious electron microscopy techniques. SRM, however, is not free from drawbacks, with the rapid quenching of the fluorescence signal, sensitivity to spherical aberrations and light scattering that typically limits imaging depth up to few micrometers being the most pronounced ones. Recently presented and robustly optimized sets of tissue optical clearing (TOC) techniques turn biological specimens transparent, which greatly increases the tissue thickness that is available for imaging without loss of resolution. Hence, SRM and TOC are naturally synergistic techniques, and a proper combination of these might promptly reveal the three-dimensional structure of entire organs with nanometer resolution. As such, an effort to introduce large-scale volumetric SRM has already started; in this review, we discuss TOC approaches that might be favorable during the preparation of SRM samples. Thus, special emphasis is put on TOC methods that enhance the preservation of fluorescence intensity, offer the homogenous distribution of molecular probes, and vastly decrease spherical aberrations. Finally, we review examples of studies in which both SRM and TOC were successfully applied to study biological systems.     

Effect of enzyme and plasma treatments of bark cloth from Ficus natalensis: morphology and thermal behavior

Bark cloth fabric has been in production in Uganda since the thirteenth century. In a move to preserve its cultural heritage, the United Nations Educational, Scientific and Cultural Organization (UNESCO) proclaimed in 2005 that Ugandan bark cloth is a “Masterpiece of the Oral and Intangible Heritage of Humanity.” Plant fibers require surface treatment before aimed at impurity reduction and for enhancement of fiber to matrix adhesion in composites. An exploratory investigation of enzymatic and plasma treatment of bark cloth is reported. The morphology of the fabric was investigated using scanning electron microscope. Thermal behavior of the fabric was studied using thermogravimetric analysis and differential scanning calorimetry. Fourier transform infrared spectroscopy and ultraviolet–visible (UV–vis) spectrophotometer were used to evaluate the surface functional groups. Enzyme-treated fabrics were cleaner and thermally stable compared to plasma and untreated fabrics.     

Comparison of characterization techniques in p-on-n HgCdTe LWIR photodiodes technology

In this paper standard techniques for characterization of HgCdTe liquid phase epitaxial layers (LPE) were presented. The performance of long wavelength p-on-n HgCdTe photodiodes fabricated by arsenic diffusion was described. The correlation between LPE HgCdTe material parameters and properties of the infrared photodiodes was demonstrated.     

Numerical simulations of the thermionic electron gun for electron-beam welding and micromachining

The paper presents numerical simulations of a hairpin thermionic electron gun, an electron source for an electron-beam welding machine. New algorithm for the simulation of emission limited by space charge has been developed and implemented in the program EOD, which enables us to simulate the optical system as whole. The results of the simulation were compared with the experimental measurements.     

Electrochromic devices employing methacrylate-based polymer electrolytes

Poly(ethyl methacrylate) (PEMA)- and poly(2-ethoxyethyl methacrylate) (PEOEMA)-based polymer gel electrolytes with entrapped solutions of lithium perchlorate in propylene carbonate (PC) were prepared by direct, UV-initiated polymerization. The electrolytes were studied using electrochemical methods and they exhibit good ionic conductivity (up to 0.7 mS cm⁻¹ at 20 °C) as well as electrochemical stability up to 2.5 V vs. Cd/Cd²⁺ (5.1 V vs. Li/Li⁺) on gold electrode. The electrolytes have thermal stability up to 125 °C. The electrolytes were successfully tested as ionic conductors in the electrochromic device FTO/WO₃/Li⁺-electrolyte/V₂O₅/FTO using coupled optoelectrochemical methods to discuss the relationship between the electrolyte composition and parameters such as change of transmittance, response time and stability. The transmittance change Δτ was found to be 30-45% at 634 nm.     

Time and Energy Performance of Parallel Systems with Hierarchical Memory

In this paper we analyze the impact of memory hierarchies on time-energy trade-off in parallel computations. Contemporary computing systems have deep memory hierarchies with significantly different speeds and power consumptions. This results in nonlinear phenomena in the processing time and energy usage emerging when the size of the computation is growing. In this paper the nonlinear dependence of the time and energy on the size of the solved problem is formalized and verified using measurements in practical computer systems. Then it is applied to formulate a problem of minimum time and minimum energy scheduling parallel processing of divisible loads. Divisible load theory is a scheduling and performance model of data-parallel applications. Mathematical programming is exploited to solve the scheduling problem. A trade-off between energy and schedule length is analyzed and again nonlinear relationships between these two criteria are observed. Further performance analysis reveals that energy consumption and schedule length are ruled by a complex interplay between the costs and speeds of on-core and out-of-core computations, communication delays, and activating new machines.     

MA‐NOTMP: A Triazacyclononane Trimethylphosphinate Based Bifunctional Chelator for Gallium Radiolabelling of Biomolecules

In the past few years, gallium‐68 has demonstrated significant potential as a radioisotope for positron emission tomography (PET), and the optimization of chelators for gallium coordination is a major goal in the development of radiopharmaceuticals. Methylaminotriazacyclononane trimethylphosphinate (MA‐NOTMP), a new C‐functionalized triazacyclononane derivative with phosphinate pendant arms, presents excellent coordination properties for ⁶⁸Ga (low ligand concentration, labelling at low pH even at room temperature). A “ready‐to‐be‐grafted” bifunctional chelating agent (p‐NCS‐Bz‐MA‐NOTMP) was prepared to allow ⁶⁸Ga labelling of sensitive biological vectors. Conjugation to a bombesin_(7–14) derivative was performed, and preliminary in vitro experiments demonstrated the potential of MA‐NOTMP in the development of radiopharmaceuticals. This new chelator is therefore of major interest for labelling sensitive biomolecules, and further in vivo experiments will soon be performed.     

3GPP LTE‐Assisted Wi‐Fi‐Direct: Trial Implementation of Live D2D Technology

This paper is a first‐hand summary on our comprehensive live trial of cellular‐assisted device‐to‐device (D2D) communications currently being ratified by the standards community for next‐generation mobile broadband networks. In our test implementation, we employ a full‐featured 3GPP LTE network deployment and augment it with all necessary support to provide real‐time D2D connectivity over emerging Wi‐Fi‐Direct (WFD) technology. As a result, our LTE‐assisted WFD D2D system enjoys the required flexibility while meeting the existing standards in every feasible detail. Further, this paper provides an account on the extensive measurement campaign conducted with our implementation. The resulting real‐world measurements from this campaign quantify the numerical effects of D2D functionality on the resultant system performance. Consequently, they shed light on the general applicability of LTE‐assisted WFD solutions and associated operational ranges.     

Effect of plasma treatment of polymeric tape carriers on wetting behaviour of aqueous ceramic tape casting slurry

Due to environmental and health aspects, aqueous ceramic slurries are preferred to traditional organic solvent systems in tape casting. An important obstacle associated with the high surface energy of water is poor wetting of aqueous ceramic slurries on polymeric tape carriers. Therefore, we measured the contact angles of an aqueous epoxy-based ceramic slurry on polyethylene terephtalate (PET), polypropylene (PP), polymethyl methacrylate (PMMA), and aluminium-coated polyethylene terephtalate (PET-Al) films and investigated approaches to improving their wetting. We evaluated the effect of plasma treatment of the tape carrier surface on the wetting behaviour and compared it with the effect of adding non-ionic amphiphilic surfactants to the ceramic slurry. The treatment of the tape carrier surface by low-temperature plasma substantially improved the wetting behaviour of aqueous ceramic slurry. The lowest contact angle of 31° was obtained on the PET film. Although the addition of non-ionic surfactants improved both the wetting behaviour of the slurry and the detachment of the polymeric carrier from the ceramic tape even better than the plasma treatment of the carrier surface did, the plasma-treated carriers still present a useful alternative to the surfactants. In the case of the plasma-treated PET carrier the surfactants could be fully eliminated and potential drawbacks related to the use of surfactants could be prevented.