Bridge condition modelling and prediction using dynamic Bayesian belief networks

The development of a condition-based deterioration modelling methodology at bridge group level using Bayesian belief network (BBN) is presented in this paper. BBN is an efficient tool to handle complex interdependencies within elements of engineering systems, by means of conditional probabilities specified on a fixed model structure. The advantages and limitations of the BBN for such applications are reviewed by analysing a sample group of masonry bridges on the UK railway infrastructure network. The proposed methodology is then extended to develop a time dependent deterioration model using a dynamic Bayesian network. The condition of elements within the selected sample of bridges and a set of conditional probabilities for static and time dependent variables, based on inspection experience, are used as input to the models to yield, in probabilistic terms, overall condition-based deterioration profiles for bridge groups. Sensitivity towards various input parameters, as well as underlying assumptions, on the point-in-time performance and the deterioration profile of the group are investigated. Together with results from ‘what if’ scenarios, the potential of the developed methodology is demonstrated in relation to the specification of structural health monitoring requirements and the prioritisation of maintenance intervention activities.     

Three-dimensional imaging of human stem cells using soft X-ray tomography

Three-dimensional imaging of human stem cells using transmission soft X-ray tomography (SXT) is presented for the first time. Major organelle types—nuclei, nucleoli, mitochondria, lysosomes and vesicles—were discriminated at approximately 50 nm spatial resolution without the use of contrast agents, on the basis of measured linear X-ray absorption coefficients and comparison of the size and shape of structures to transmission electron microscopy (TEM) images. In addition, SXT was used to visualize the distribution of a cell surface protein using gold-labelled antibody staining. We present the strengths of SXT, which include excellent spatial resolution (intermediate between that of TEM and light microscopy), the lack of the requirement for fixative or contrast agent that might perturb cellular morphology or produce imaging artefacts, and the ability to produce three-dimensional images of cells without microtome sectioning. Possible applications to studying the differentiation of human stem cells are discussed.     

Analytical and Numerical Modeling of Soft Soil Stabilized by Prefabricated Vertical Drains Incorporating Vacuum Preloading

This paper describes the analytical formulation of a modified consolidation theory incorporating vacuum pressure, and numerical modeling of soft clay stabilized by prefabricated vertical drains, with a linearly distributed (trapezoidal) vacuum pressure for both axisymmetric and plane strain conditions. The effects of the magnitude and distribution of vacuum pressure on soft clay consolidation are examined through average time-dependent excess pore pressure and consolidation settlement analyses. The plane strain analysis was executed by transforming the actual vertical drains into a system of equivalent parallel drain walls by adjusting the coefficient of permeability of the soil and the applied vacuum pressure. The converted parameters are incorporated in the finite element code ABAQUS, employing the modified Cam-clay theory. Numerical analysis is conducted to study the performance of a full-scale test embankment constructed on soft Bangkok clay. The performance of this selected embankment is predicted on the basis of four different vacuum pressure distributions. The predictions are compared with the available field data. The assumption of distributing the vacuum pressure as a constant over the soil surface and varying it linearly along the drains seems justified in relation to the field data.     

From oogonia to mature oocytes: inactivation of the maternal centrosome in humans

The fine structure of human oogonia and growing oocytes has been reviewed in fetal and adult ovaries. Preovulatory maturation and the ultrastructure of stimulated oocytes from the germinal vesicle (GV) stage to metaphase II (MII) stage are also documented. Oogonia have large nuclei, scanty cytoplasm with complex mitochondria. During folliculogenesis, follicle cell processes establish desmosomes and deep gap junctions at the surface of growing oocytes, which are retracted during the final stages of maturation. The zona pellucida is secreted in secondary follicles. Growing oocytes have mitochondria, Golgi, rough endoplasmic reticulum (RER), ribosomes, lysosomes, and lipofuscin bodies, often associated with Balbiani bodies and have nuclei with reticulated nucleoli. Oocytes from antral follicles show numerous surface microvilli and cortical granules (CGs) separated from the oolemma by a band of microfilaments. The CGs are evidently secreted by Golgi membranes. The GV oocytes have peripheral Golgi complexes associated with a single layer of CGs close to the oolemma. They have many lysosomes, and nuclei with dense compact nucleoli. GV breakdown occurs by disorganization of the nuclear envelope and the oocyte enters a transient metaphase I followed by MII, when it is arrested and ovulated. Maturation of oocytes in vitro follows the same pattern of meiosis seen in preovulatory oocytes. The general organization of the human oocyte conforms to that of most other mammals but has some unique features. The MII oocyte has the basic cellular organelles such as mitochondria, smooth endoplasmic reticulum, microfilaments, and microtubules, while Golgi, RER, lysosomes, multivesicular, residual and lipofuscin bodies are very rare. It neither has yolk nor lipid inclusions. Its surface has few microvilli, and 1-3 layers of CGs, aligned beneath the oolemma. Special reference has been made to the reduction and inactivation of the maternal centrosome during oogenesis. The MII spindle, often oriented perpendicular to the oocyte surface, is barrel-shaped, anastral and lacks centrioles. Osmiophilic centrosomes are not demonstrable in human eggs, since the maternal centrosome is nonfunctional. However, oogonia and growing oocytes have typical centrioles, similar to those of somatic cells. The sperm centrosome activates the egg and organizes the sperm aster and mitotic spindles of the embryo, after fertilization.     

Screening and Evaluation of Polyhydroxybutyrate-Producing Strains from Indigenous Isolate Cupriavidus taiwanensis Strains

Polyhydroxyalkanoate (PHA) is a biodegradable material with many potential biomedical applications, including medical implants and drug delivery. This study developed a system for screening production strains in order to optimize PHA production in Cupriavidus taiwanensis 184, 185, 186, 187, 204, 208, 209 and Pseudomona oleovorans ATCC 29347. In this study, Sudan black B staining, Infrared (IR) and Gas Chromatography (GC) analysis indicated that the best strain for PHA synthesis is C. taiwanensis 184, which obtains polyhydroxybutyrate (PHB). Cultivation of C. taiwanensis 184 under a pH of 7.0, at 30 °C, and at an agitation rate of 200 rpm, obtained a PHB content of 10% and PHB production of 0.14 g/L. The carbon and nitrogen types selected for analysis of PHB production by C. taiwanensis 184 were gluconic acid and NH(4)Cl, respectively. Optimal carbon/nitrogen ratio for PHB production was also determined. This study demonstrated a PHB content of 58.81% and a PHB production of 2.44 g/L when the carbon/nitrogen ratio of 8/1 was selected for C. taiwanensis 184. A two-stage fermentation strategy significantly enhanced PHB content and PHB production. Under a two-stage fermentation strategy with nutrient-limited conditions, C. taiwanensis 184 obtained a PHB content of 72% and a PHB concentration of 7 g/L. Finally, experimental results confirmed that optimizing the growth medium and fermentation conditions for cultivating the indigenous C. taiwanensis 184 strain substantially elevated PHB content from 10% to 72% and PHB production from 0.14 g/L to 7 g/L, respectively.     

Synthesis of substituted poly (arylene vinylene) films by vapor deposition polymerization

Summary An efficient method for the synthesis of PPV based polymers, poly ( 2,5-dimethyl-1,4-phenylene vinylene) (DMe-PPV) and poly (2,5-dimethoxy-1,4-phenylene vinylene) (DOMe-PPV) were developed using 2,5-dimethyl-1,4-bis ( chloromethyl ) benzene and 2,5-dimethoxy 1,4-bis ( chloromethyl ) benzene via vapor phase pyrolysis and followed by vapor deposition polymerization. The structure of polymer films were confirmed by FT-IR, solid state NMR and elemental analysis. Thermal gravimetry analysis reveals that the precursor polymer films form a conjugated polymer after thermal conversion at 250°C. The optical and electrical properties of the polymer films were investigated by UV-Vis absorption and photoluminescence spectroscopies. Electroluminescent devices were fabricated using these polymers. Received: 18 May 2000/Revised version: 21 July 2000/Accepted: 28 July 2000     

Formalizing real-time scheduling using priority-based supervisory control of discrete-event systems

In this note, we formalize real-time task scheduling by applying an extension of supervisory control theory (SCT) of discrete-event systems to real-time models. The set of all possible timed traces of the system is specified by a discrete timed automaton where each transition is associated with an event occurrence or the passage of one unit of time. We introduce priorities to SCT, and apply them to the setting of discrete timed automata in order to develop a formal and unified framework for task scheduling on a single CPU.     

Ontogenesis of textile face mask using cotton fabric by treating with red seaweeds extract for cosmetotextile applications

This study investigated the effective utilization of phenolic compounds present in red seaweeds such as Kappaphycus alvarezii and Acanthophora spicifera used for making textile face masks for anti-aging applications. To study the presence of functional and antioxidant compounds in the red seaweeds was assessed using Fourier transform infrared spectroscopy, and 2-2-diphenyl-1-picrylhydrazyl free radical scavenging methods. The effect of red seaweed treated fabrics interaction with fibroblast populated collagen (FPC) layers was assessed using topographical analysis of field emission scanning electron microscope and the effect of red seaweed treated textile fabrics on comfort properties such as air permeability, water permeability, and wicking properties were studied. The measurement and analysis of FPC layer interaction with textile fabrics were assessed using contraction studies. The experimental result specifies that maximum antioxidant activity of 77 ± 0.17% inhibition achieved in the textile face mask. The air permeability, wickability and water permeable properties were slightly reduced for both red seaweed treated textile face mask fabrics compared with the untreated face mask.     

Spray dried solid dispersions of piroxicam in carboxymethyl sago cellulose using aqueous solvents: A simple,novel and green approach to produce enteric microparticles with enhanced dissolution

Piroxicam-CMSC solid dispersions were produced by spray drying from aqueous solvents. Depending on the drug-polymer ratio, loading and entrapment efficiency of CMSC microparticles were 6.8–46.75 and 40.79–60.35% w/w, respectively. Scanning electron microscopy revealed non-spherical geometry and agglomeration of the spray-dried particles. The average size of the particles ranged from 7 to 170?µm. Infrared spectroscopy, differential scanning calorimetry, and X-ray diffraction confirmed intact crystalline piroxicam in the microparticles. In gastric pH, microparticles and native piroxicam have shown less than 25% and more than 45% of drug dissolution, respectively in 2?h. In contrast, at pH 7.4, microparticles have shown 80% of drug dissolution; whereas native piroxicam achieved only 30% of dissolution by 30?min. The spray-dried CMSC particles are efficient in restricting drug release in gastric pH and enhance drug dissolution in intestinal pH. The method is eco-friendly as it uses aqueous solvents and non-toxic materials.     

Carboxymethyl sago pulp/carboxymethyl sago starch hydrogel: Effect of polymer mixing ratio and study of controlled drug release

Carboxymethyl sag o pulp (CMSP)/carboxymethyl sago starch (CMSS) hydrogel was synthesized by electron beam irradiation. In the series of hydrogels prepared, 40%/20% CMSP/CMSS hydrogel had the highest gel fraction. The swelling capacity of CMSP/CMSS hydrogel was found to be highest in distilled water, followed by pH 11, pH 7.4, and pH 1.2. Scanning Electron Microscope photographs revealed that the drug‐loaded hydrogel had a smoother surface than unloaded hydrogel. Fourier Transform Infrared and Differential Scanning Calorimetry analysis showed the absence of interaction between the hydrogels and the drug. All drug‐loaded hydrogels had drug encapsulation efficiency between 63% and 69%. CMSP/CMSS hydrogel swelled and allowed the release of drug at pH 7.4. These properties qualify the hydrogel as a potential candidate for controlled drug release at the ocular and colonic regions. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016 , 133, 43652.