pH titration of native and unfolded beta-trypsin: evaluation of the delta delta G0 titration and the carboxyl pK values

The stabilizing free energy of beta-trypsin was determined by hydrogen ion titration. In the pH range from 3.0 to 7.0, the change in free energy difference for the stabilization of the native protein relative to the unfolded one (delta delta G0 titration) was 9.51 +/- 0.06 kcal/mol. An isoelectric point of 10.0 was determined, allowing us to calculate the Tanford and Kirkwood electrostatic factor w. This factor presented a nonlinear behavior and indicated more than one type of titratable carboxyl groups in beta-trypsin. In fact, one class of carboxyl group with a pK = 3.91 +/- 0.01 and another one with a pK = 4.63 +/- 0.03 were also found by hydrogen ion titration of the protein in the folded state.     

Speed reduction on through roads in Nordrhein-Westfalen.

The paper describes a program of experiments for speed reduction on 28 through roads in Nordrhein-Westfalen. In the process, different measures of construction, design, and traffic control were examined to determine their usefulness to reduce speed and to improve road safety. The need for systematic evaluation of a catalogue of measures partly resulted in unsatisfactory design conceptions for the through roads. The evaluation of the effects of the program has been carried out by use of before-and-after-studies of large extent. The speed measurements show that a distinct speed reduction can be obtained only from selected physical improvements. Measures based on optical effects and traffic signs were found to have very little effect on vehicle speeds. Furthermore, in light of the relatively small number of accidents that occur on the selected facilities, it was not possible to establish the beneficial impact of each specific control measure with statistical certainty. However, the results of the experiment have had an influence on the current definition of new design standards for main urban streets in Germany.     

Zinc-specific food frequency questionnaire.

PURPOSE: A new dietary zinc assessment tool (ZAT) was evaluated to determine its usefulness in estimating zinc intakes among college students. METHODS: A food frequency questionnaire specifically designed for calculating average zinc intakes was administered to university students who had also completed three-day food records. Zinc intakes from the two instruments were compared. RESULTS: Among 171 participants (38 male, 133 female) aged 20.2 +/- 1.6 years (mean +/- standard deviation), the two dietary assessment methods were positively correlated (r=0.33, p<0.001). The ZAT correctly identified 76% of the women who were obtaining less than the National Academy of Sciences Recommended Dietary Allowance for zinc (8 mg/day for females, 11 mg/day for males). CONCLUSIONS: This convenient tool may assist in the identification of problematic dietary patterns at an early stage. Further design modifications and expanded studies are warranted.     

The occurrence of goitrogenic substances in milk. 1. Release of goitrin in the milk of cows fed on rapeseed extract cakes

A total of six cows, divided into 3 groups, were fed various amounts of rape cake containing 6 g of goitrin/kg over a period of 7 days. The cows were milked twice a day and the goitrin content of the heated milk samples were determined by a HPLC-method within 2 h. When rape cake was fed at 0.39, 1.9 and 3.9% resp. of the total feed this resulted in medium goitrin values of 37, 163 and 707 micrograms/l milk. These values correspond to a transfer of about 0.1% of the original progoitrin content in the feed. 12 h after the last rape feeding the amount of goitrin in the milk was below the detection limit of 7 ppb. The toxicological significance of these findings are dicussed.     

Bestimmung von Haselnußprotein (Corylin) mit Hilfe der Elektroimmundiffusion nach Laurell

European Food Research and Technology - Bei Bestimmungen des Proteingehalts von Haselnüssen in verschiedenen Handelsprodukten wie Nuß-Nougatcremes and Nußmus mit der...     

堤坝渗漏防治技术在社岗防护堤的应用

该文从设计、施工和运行管理等方面介绍了高喷板墙、减压井、充填灌浆以及开挖导渗等技术在飞来峡水利枢纽社岗防护堤中的应用情况,探讨了堤坝渗漏综合防治的技术措施和有关问题。