Nonlinear analysis for seismic upgrade studies of existing essential facilities

The need to perform cost-effective seismic upgrades of essential facilities has made the use of nonlinear finite-element techniques an attractive option. These techniques allow for the analysis of full buildings with large, nonlinear deformations. For reinforced concrete structures, this includes concrete crushing and cracking, and steel reinforcement yielding. These nonlinear models can predict the response of buildings subjected to strong motion earthquakes with a greater degree of accuracy than simple linear or ‘quasi’ nonlinear models.     

Measurement of three independent components in impedance sensors using a single square wave

Two-wire impedance-based sensors involving electrolytes add the impedance of the electrodes to the electrical impedance of the medium to measure. An equivalent circuit for the measured impedance is a resistance in series with the parallel combination of another resistance and a capacitance. If the two electrodes are modelled by equal impedances, the equivalent circuit for the complete set up consists of three impedance components, which can be determined from three independent measurements. This paper describes a novel method to obtain those three components using a single square wave voltage (period 2T) instead of several sine waves and provides the equations to calculate their value from the three current intensity amplitudes measured at T/8, 3T/8 and 5T/8. Other measurement times would need different equations, but the same procedure applies. Anyway, the proposed method keeps the advantages of synchronous detection and relies on analytical solutions instead of the customary curve fitting procedures. Computer simulation and experimental results obtained by measuring the conductivity of known electrolyte samples validate the proposed method.     

A Color Vision-Based Lane Tracking System for Autonomous Driving on Unmarked Roads

This work describes a color Vision-based System intended to perform stable autonomous driving on unmarked roads. Accordingly, this implies the development of an accurate road surface detection system that ensures vehicle stability. Although this topic has already been documented in the technical literature by different research groups, the vast majority of the already existing Intelligent Transportation Systems are devoted to assisted driving of vehicles on marked extra urban roads and highways. The complete system was tested on the BABIECA prototype vehicle, which was autonomously driven for hundred of kilometers accomplishing different navigation missions on a private circuit that emulates an urban quarter. During the tests, the navigation system demonstrated its robustness with regard to shadows, road texture, and weather and changing illumination conditions.     

Packet Video Broadcasting with General-Purpose Operating Systems in an Ethernet

Video transmission with general-purpose PCs poses a number of requirements that radically differ from those of high-end dedicated video servers. We analyze the scenario of an Ethernet local area network in which a number of PCs are transmitting video streams, while other TCP/IP applications are also running concurrently. Our findings show that since the operating system clock resolution cannot cope with the transmission timing requirements the following holds: if the video transmission is performed with exact timing accuracy to maintain a constant rate then CPU load grows to 100%, thus blocking the PC for other user applications; on the other hand, if transmission is performed in a bursty manner, i.e. with sleep system calls, then CPU load decreases dramatically but the increased burstiness of the video stream has a negative impact on network performance (for example, capture effect in the Ethernet). Furthermore, the impact of video transmission over the rest of TCP/IP applications running on the same network depends heavily on the packet size. We provide an integrated analysis of operating system and network parameters to achieve video broadcasting while preserving timing requirements and minimizing the impact on other applications.     

Development and Characterization of a Factor V-Deficient CRISPR Cell Model for the Correction of Mutations

Factor V deficiency, an ultra-rare congenital coagulopathy, is characterized by bleeding episodes that may be more or less intense as a function of the levels of coagulation factor activity present in plasma. Fresh-frozen plasma, often used to treat patients with factor V deficiency, is a scarcely effective palliative therapy with no specificity to the disease. CRISPR/Cas9-mediated gene editing, following precise deletion by non-homologous end-joining, has proven to be highly effective for modeling on a HepG2 cell line a mutation similar to the one detected in the factor V-deficient patient analyzed in this study, thus simulating the pathological phenotype. Additional CRISPR/Cas9-driven non-homologous end-joining precision deletion steps allowed correction of 41% of the factor V gene mutated cells, giving rise to a newly developed functional protein. Taking into account the plasma concentrations corresponding to the different levels of severity of factor V deficiency, it may be argued that the correction achieved in this study could, in ideal conditions, be sufficient to turn a severe phenotype into a mild or asymptomatic one.     

Technical Validation and Clinical Implications of Ultrasensitive PCR Approaches for EGFR-Thr790Met Mutation Detection in Pretreatment FFPE Samples and in Liquid Biopsies from Non-Small Cell Lung Cancer Patients

In pretreatment tumor samples of EGFR-mutated non-small cell lung cancer (NSCLC) patients, EGFR-Thr790Met mutation has been detected in a variable prevalence by different ultrasensitive assays with controversial prognostic value. Furthermore, its detection in liquid biopsy (LB) samples remains challenging, being hampered by the shortage of circulating tumor DNA (ctDNA). Here, we describe the technical validation and clinical implications of a real-time PCR with peptide nucleic acid (PNA-Clamp) and digital droplet PCR (ddPCR) for EGFR-Thr790Met detection in diagnosis FFPE samples and in LB. Limit of blank (LOB) and limit of detection (LOD) were established by analyzing negative and low variant allele frequency (VAF) FFPE and LB specimens. In a cohort of 78 FFPE samples, both techniques showed an overall agreement (OA) of 94.20%. EGFR-Thr790Met was detected in 26.47% of cases and was associated with better progression-free survival (PFS) (16.83 ± 7.76 vs. 11.47 ± 1.83 months; p = 0.047). In LB, ddPCR was implemented in routine diagnostics under UNE-EN ISO 15189:2013 accreditation, increasing the detection rate of 32.43% by conventional methods up to 45.95%. During follow-up, ddPCR detected EGFR-Thr790Met up to 7 months before radiological progression. Extensively validated ultrasensitive assays might decipher the utility of pretreatment EGFR-Thr790Met and improve its detection rate in LB studies, even anticipating radiological progression.     

Special issue on distributed computing and artificial intelligence

4:1! Google’s artificial intelligence (AI) program, AlphaGo, has won Go Master Lee Sedol in a best-of-five competition held in Korean March 9?15, 2016. Seen by many as a landmark moment for AI, the outcome did not come as a surprise, considering the excellent combination of 1920 CPUs with sophisticated AI algorithms, including neural networks and Monte Carlo tree search (Gibney, 2016; Silver et al., 2016). Indeed, research on distributed computing and artificial intelligence (DCAI) has matured during the last decade and many effective applications are now deployed, performing an increasingly important role in modern computer science, including the two most hyped technologies: Internet of Things and Big Data. Indeed, it is fair to say that the application of artificial intelligence in distributed environments is becoming an essential element of high added value and economic potential.     

Dynamic modeling of a dual active bridge DC to DC converter with average current control and load‐current feed‐forward

Bidirectional power flow is needed in many power conversion systems like energy storage systems, regeneration systems, power converters for improvement of the power quality and some DC‐DC applications where bidirectional high power conversion and galvanic isolation are required. The dual active bridge (DAB) is an isolated, high voltage ratio DC‐DC converter suitable for high power density and high power applications, being a key interface between renewable energy sources and energy storage devices. This paper is focused on the modeling and control design of a DC‐DC system with battery storage based on a DAB converter with average current mode control of the output current and output voltage control. The dynamic response of the output voltage to load steps is improved by means of an additional load‐current feed‐forward control loop. An analytical study of the load‐current feed‐forward is presented and validated by means of both simulations and experimental results. Copyright © 2014 John Wiley & Sons, Ltd.     

BETaaS: A Platform for Development and Execution of Machine-to-Machine Applications in the Internet of Things

Wireless Personal Communications - The integration of everyday objects into the Internet represents the foundation of the forthcoming Internet of Things (IoT). Smart objects will be the building...     

Critical Review on Fatty Acid-Based Food and Nutraceuticals as Supporting Therapy in Cancer

Fatty acids have an important place in both biological and nutritional contexts and, from a clinical point of view, they have known consequences for diseases’ onset and development, including cancer. The use of fatty acid-based food and nutraceuticals to support cancer therapy is a multidisciplinary subject, involving molecular and clinical research. Knowledge regarding polyunsaturated fatty acids essentiality/oxidizability and the role of lipogenesis-desaturase pathways for cell growth, as well as oxidative reactivity in cancer cells, are discussed, since they can drive the choice of fatty acids using their multiple roles to support antitumoral drug activity. The central role of membrane fatty acid composition is highlighted for the application of membrane lipid therapy. As fatty acids are also known as biomarkers of cancer onset and progression, the personalization of the fatty acid-based therapy is also possible, taking into account other important factors such as formulation, bioavailability and the distribution of the supplementation. A holistic approach emerges combining nutra- and pharma-strategies in an appropriate manner, to develop further knowledge and applications in cancer therapy.