The carbohydrate and aromatic amino acid metabolism of several species related to the human colon was investigated into more detail. Therefore, in vitro fermentations were performed, with different carbohydrate sources, during which several aromatic amino acids were added to the fermentation medium. Shifts in end-product formation in response to the available nutrients were observed for all strains tested. The major part of amino acid degradation occurred after depletion of the carbohydrates. Moreover, it was shown that Bifidobacterium strains are capable of degrading aromatic amino acids in the absence of carbohydrates. The excretion of certain intermediates of the aromatic amino acid metabolism was observed for a strain of Clostridium clostridioforme, after which they were metabolized again during a later stage of fermentation. This implies that cross-feeding on degradation products of aromatic amino acids, albeit within the same species, can occur in the human colon. 相似文献
The present study describes the effects of glycerol, relative humidity (RH) and the minor components of corn flour on mechanical and thermal properties of native flour, defatted flour, and starch‐based materials. The kinetic of retrogradation for these different materials were dissimilar. For all samples, strain at break shows a maximum value as a function of RH followed by a decrease, explained by the appearance of water and glycerol clusters. Starch controls the mechanical properties of corn flour‐based material and their variations with temperature and humidity. Lipid and protein have negative effect on mechanical properties of thermoplastic flour toward starch but they did not have a plasticizing effect on the physicochemical behavior of the matrix. Tgs decreased as a result of water content increase. 相似文献
The literature is reviewed on the formation and the structure of β-lactoglobulin and whey protein isolate (WPI) aggregates in aqueous solution induced by heating. The focus is on the effects of the pH and added salt. The use of β-lactoglobulin and WPI aggregates in cold-set gels, foams and emulsions, encapsulation, and films and coatings is also reviewed. 相似文献
Hazelnuts have been shown to contain different allergenic proteins. Amongst these, major allergens Cor a 1 and Cor a 8 and the 2S albumin Cor a 14 were selected as targets to comparatively validate three Real-Time PCR protocols. We investigated both on the choice of the amplification target, and on the matrix effect on different sample foods. Applying statistics on the validation parameters obtained from the three protocols, we showed a significant difference in Ct values. This could turn critical when a high sensitivity method is required for the detection of hazelnut traces, confirming how fundamental the choice of the template during primer design phase is. Concluding, statistical approach represents a useful tool for the identification of the best performing primer pairs in Real-Time PCR. Cor a 8 gene permitted the identification of hazelnut based ingredients in complex foods, providing a significantly higher sensitivity in the PCR amplification, when compared to Cor a 1 and Cor a 14. 相似文献
The effect of Co, Pd and Pt ultrathin films on the kinetics of the formation of Ni-silicide by reactive diffusion is investigated. 50 nm Ni/1 nm X/ 50 nm Ni (X?=?Co, Pd, Pt) deposited on Si(100) substrates are studied using in-situ and ex-situ measurements by X-ray diffraction (XRD). The presence of Co, Pd or Pt thin films in between the Ni layers delays the formation of the metal rich phase compared to the pure Ni/Si system and thus these films act as diffusion barriers. A simultaneous silicide formation (δ-Ni2Si and NiSi phases) different from the classic sequential formation is found during the consumption of the top Ni layer for which Ni has to diffuse through the barrier. A model for the simultaneous growth in the presence of a barrier is developed, and simulation of the kinetics measured by XRD is used to determine the permeability of the different barriers. Atom probe tomography (APT) of the Ni/Pd/Ni system shows that the Pd layer is located between the Ni top layer and δ-Ni2Si during the silicide growth, in accordance with a silicide formation controlled by Ni diffusion through the Pd layer. The effect of the barrier on the silicide formation and properties is discussed.
This paper characterizes stress levels via a self‐similarity analysis of the electrodermal activity (EDA) collected in a real‐world driving context. To characterize the EDA richness over scales, the fractional Brownian motion (FBM) process and its corresponding exponent H, estimated via a wavelet‐based approach, are used. Specifically, an automatic scale range selection is proposed in order to detect the linearity in a log scale diagram. The procedure is applied to the EDA signals, from the open database drivedb, originally captured on the foot and the hand of the drivers during a real‐world driving experiment, designed to evoke different levels of arousal and stress. The estimated Hurst exponent H offers a distinction in stress levels when driving in highway versus city, with a reference to restful state of minimal stress level. Specifically, the estimated H values tend to decrease when the driving environmental complexity increases. In addition, the estimated H values on the foot EDA signals allow a better characterization of the driving task than that of hand EDA. The self‐similarity analysis was applied to various physiological signals in literature but not to the EDA so far, a signal which was found to correlate most with human affect. The proposed analysis could be useful in real‐time monitoring of stress levels in urban driving spaces, among other applications. 相似文献