Bio-cellular processes modeling on silicon substrate: receptor–ligand binding and Michaelis Menten reaction

Reducing transmit power is the most straightforward way towards more energy-efficient communications, but it results in lower SNRs at the receiver which can add a performance and/or complexity cost. At low SNRs, synchronization and channel estimation errors erode much of the gains achieved through powerful turbo and LDPC codes. Further expanding the turbo concept through an iterative receiver—which brings synchronization and equalization modules inside the loop—can help, but this solution is prohibitively complex and it is not clear what can and what cannot be a part of the iterative structure. This paper fills two important gaps in this field: (1) as compared to previous research which either focuses on a subset of the problem assuming perfect remaining parameters or is computationally too complex, we propose a proper partitioning of algorithm blocks in the iterative receiver for manageable delay and complexity, and (2) to the best of our knowledge, this is the first physical demonstration of an iterative receiver based on experimental radio hardware. We have found that for such a receiver to work, (1) iterative timing synchronization is impractical, iterative carrier synchronization can be avoided by using our proposed approach, while iterative channel estimation is essential, and (2) the SNR gains claimed in previous publications are validated in indoor channels. Finally, we propose a heuristic algorithm for simplifying the carrier phase synchronization in an iterative receiver such that computations of the log likelihood ratios of the parity bits can be avoided to strike a tradeoff between complexity and performance.     

Pacemaker interference by magnetic fields at power line frequencies

Human exposure to external 50/60-Hz electric and magnetic fields induces electric fields within the body. These induced fields can cause interference with implanted pacemakers. In the case of exposure to magnetic fields, the pacemaker leads are subject to induced electromotive forces, with current return paths being provided by the conducting body tissues. Modern computing resources used in conjunction with millimeter-scale human body conductivity models make numerical modeling a viable technique for examining any such interference. In this paper, an existing well-verified scalar-potential finite-difference frequency-domain code is modified to handle thin conducting wires embedded in the body. The effects of each wire can be included numerically by a simple modification to the existing code. Results are computed for two pacemaker lead insertion paths, terminating at either atrial or ventricular electrodes in the heart. Computations are performed for three orthogonal 60-Hz magnetic field orientations. Comparison with simplified estimates from Faraday's law applied directly to extracorporeal loops representing unipolar leads underscores problems associated with this simplified approach. Numerically estimated electromagnetic interference (EMI) levels under the worst case scenarios are about 40 microT for atrial electrodes, and 140 microT for ventricular electrodes. These methods could also be applied to studying EMI with other implanted devices such as cardiac defibrillators.     

Characteristics of the heterologously expressed human lanosterol 14α‐demethylase (other names: P45014DM,CYP51, P45051) and inhibition of the purified human and Candida albicans CYP51 with azole antifungal agents

Human and Candida albicans CYP51 were purified to homogeneity after GAL10‐based heterologous expression in yeast in order to resolve the basis for the selective inhibition of the fungal enzyme over the human orthologue by the azole drugs ketoconazole and itraconazole, used in the treatment of systemic fungal infection. The purified proteins have similar spectral characteristics, both giving a maximum at 448 nm in reduced carbon monoxide difference spectra. Substrate affinity constants of 20·8 and 29·4 μM and V_max of 0·15 and 0·47 nmol/min/nmol were observed for C. albicans and human enzymes, respectively, in reconstituted enzymatic assays, using an intermediate of the demethylation reaction [32‐³H]‐3β‐hydroxylanost‐7‐en‐32‐ol as the substrate. Both enzymes gave similar type II spectra on titration with drugs, but a reduced affinity was observed for human CYP51 using the ability of carbon monoxide to displace the drug as a ligand and by calculation of IC₅₀. However, although the results indicate higher affinity of the drugs for their target CYP51 in the major fungal pathogen C. albicans, when compared directly to CYP51 from humans, the difference was less than 10‐fold. This difference is an order of magnitude lower than previously reported data based on measurements using unpurified human CYP51 enzyme preparations. Consequently, increased azole doses to combat resistant candidaemia may well inhibit endogenous human CYP51 and the potential consequences are discussed. Copyright © 1999 John Wiley & Sons, Ltd.     

Immunocytochemical study of alpha 1 and beta 2/3 subunits of GABAA receptors in freehand isolated vestibular Deiters' neurons

Vestibular Deiters' neurons have been isolated from bovine brain by the Hydén's freehand dissection technique and challenged with monoclonal antibodies directed toward the alpha 1 and beta 2/3 subunits of the GABAA receptors. Subsequent challenge with fluorescent secondary antibodies and confocal microscopy allowed the study of the cellular distribution of such subunits. In Deiters' neurons the beta 2/3 subunit displayed a clear presence all along the cell body profile and the initial parts of the dendrites. The alpha 1 subunit was found highly present all over the cell interior except the nuclear profiles. The strong presence inside the cells possibly masked its presence on the plasma membrane. However, in part of the cells studied a distinct presence on the plasma membrane was evident. This subunit was visualized also all along the long dendrites of these neurons. The approach we describe here, involving freehand isolated mature neurons from adult animals, may allow a better characterization of the tridimensional distribution of different types of neuronal GABAA receptors in the respect of the approach with brain slices.     

Evaluation of signalling loads in a cognitive network management architecture

Future networks will need to accommodate a significantly augmented user demand, mainly stemming from the wireless and mobile domains. In general, the emerging radio landscape will comprise multiple, collaborating radio access networks (RANs) able to operate a plethora of diverse radio access technologies (RATs), variant types of mobile terminals (MTs), with the ability to choose among various supported RANs/RATs and, in addition, both devices and networks with dynamic spectrum access capabilities that allow the sharing and/or optimization of spectrum usage among different systems. The above will stress network operators for developing mechanisms to confront the challenges and to leverage the opportunities posed by such a versatile radio environment. In particular, the situation calls for adaptive and flexible management paradigms that are able to dynamically manage network elements and terminals, thus ensuring the great availability and efficient usage of spectrum and other radio resources. Framed within the above, this paper considers a cognitive network management architecture, which is destined for optimized management of future wireless networks operating in versatile radio environments, and presents a performance evaluation methodology, which was set up for measuring the signalling loads that the operation of the architecture will bring to the managed network. The methodology is analytically described, and useful results with respect to the signalling load produced for management signalling purposes in an indicative scenario are presented and analysed. Copyright © 2011 John Wiley & Sons, Ltd.     

Integration of InGaP/GaAs/Ge triple‐junction solar cells on deeply patterned silicon substrates

We report preliminary results on InGaP/InGaAs/Ge photovoltaic cells for concentrated terrestrial applications, monolithically integrated on engineered Si(001) substrates. Cells deposited on planar Ge/Si(001) epilayers, grown by plasma‐enhanced chemical vapor deposition, provide good efficiency and spectral response, despite the small thickness of the Ge epilayers and a threading dislocation density as large as 10⁷/cm². The presence of microcracks generated by the thermal misfit is compensated by a dense collection grid that avoids insulated areas. In order to avoid the excessive shadowing introduced by the use of a dense grid, the crack density needs to be lowered. Here, we show that deep patterning of the Si substrate in blocks can be an option, provided that a continuous Ge layer is formed at the top, and it is suitably planarized before the metalorganic chemical vapor deposition. The crack density is effectively decreased, despite that the efficiency is also lowered with respect to unpatterned devices. The reasons of this efficiency reduction are discussed, and a strategy for improvement is proposed and explored. Full morphological analysis of the coalesced Ge blocks is reported, and the final devices are tested under concentrated AM1.5D spectrum. Copyright © 2016 John Wiley & Sons, Ltd.     

An electronically tunable microstrip bandpass filter using thin-film Barium-Strontium-Titanate (BST) varactors

A tunable third-order combline bandpass filter using thin-film barium-strontium-titanate varactors and fabricated on a sapphire substrate is reported. Application of 0-200-V bias varied the center frequency of the filter from 2.44 to 2.88 GHz (16% tuning) while achieving a 1-dB bandwidth of 400 MHz. The insertion loss varied from 5.1 dB at zero bias to 3.3 dB at full bias, while the return loss exceeded 13 dB over the range. The third-order intercept of the filter was found to be 41 dBm.     

Network and single user performance evaluation of a mobile data system over flat fading transmission channels

In this paper the contrasting effects of transmission impairments and capture on both the network and single user performance of a slotted Aloha system are investigated in a mobile radio environment, accounting for frequency non-selective random propagation phenomena, and employing the packet error probability in order to define packet losses and capture. With this study we demonstrate that it is possible to generalize in a real propagation context a method previously proposed in literature for evaluating the network behavior in terms of steady-state throughput, backlog and stability in conventional transmission conditions, i.e., when all the transmission channels were error-free and the collisions caused the loss of all the packets involved. Moreover, we indicate under which specific constraints on the terminal mobility we can apply this method to analytically predict the single user performance, which we show being an important design parameter. Through the numerical results reported we quantitatively point out that in the absence of coding capture increases system stability and moderately improves the overall system throughput and backlog. We also outline the trade-off between an increased capture gain obtained by means of coding and the corresponding system cost in terms of complexity and bandwidth occupancy. Furthermore, we demonstrate the unfairness which affects the single user performance and the consequent need for countermeasures in order not to discriminate among users differently located within the network; yet, this last solution is detrimental as regards the positive capture effects.This work was supported by M.U.R.S.T. and C.N.R. (Italy).     

Chloride and Indium‐Chloride‐Complex Inorganic Ligands for Efficient Stabilization of Nanocrystals in Solution and Doping of Nanocrystal Solids

Here, the surface functionalization of CdSe and CdSe/CdS core/shell nanocrystals (NCs) with compact chloride and indium‐chloride‐complex ligands is reported. The ligands provide not only short interparticle distances but additionally control doping and passivation of surface trap states, leading to enhanced electronic coupling in NC‐based arrays. The solids based on these NCs show an excellent electronic transport behavior after heat treatment at the relatively low temperature of 190 °C. Indeed, the indium‐chlorido‐capped 4.5 nm CdSe NC based thin‐film field‐effect transistor reaches a saturation mobility of μ = 4.1 cm² (V s)^?1 accompanied by a low hysteresis, while retaining the typical features of strongly quantum confined semiconductor NCs. The capping with chloride ions preserves the high photoluminescence quantum yield ( ≈ 66%) of CdSe/CdS core/shell NCs even when the CdS shell is relatively thin (six monolayers). The simplicity of the chemical incorporation of chlorine and indium species via solution ligand exchange, the efficient electronic passivation of the NC surface, as well as their high stability as dispersions make these materials especially attractive for wide‐area solution‐processable fabrication of NC‐based devices.