On heat transfer characteristics of real and simulant melt pool experiments

This paper presents results of analytical studies on natural convection heat transfer in scaled and/or simulant melt pool experiments related to the pressurized water reactor in-vessel melt retention issue. Specific reactor-scale effects of a large decay-heated core melt pool in the reactor pressure vessel lower plenum are first reviewed, and then the current analytical capability of describing the relevant physical processes in prototypical situations is examined. Experiments and experimental approaches are analyzed by focusing on their ability to represent prototypical situations. Calculations are performed to assess the significance of some selected effects, including variations in melt properties, pool geometry and heating conditions. In the present analysis, Rayleigh numbers are limited to 10¹², where uncertainties in turbulence modelling do not override other uncertainties. Calculations are performed to explore limitations of using side wall heating and direct electrical heating. The need for further experimental and analytical efforts is also discussed.     

5,6-Dichloro-1-Beta-D-ribofuranosylbenzimidazole inhibits initiation of nuclear heterogeneous RNA chains in HeLa cells

The nucleoside analog 5, 6-dichloro-1-beta-d-ribofuranosylbenzimidazole (DRB) at 75 to 150 micromolar concentrations inhibits the synthesis of nuclear heterogeneous RNA (hnRNA) in HeLa cells by 60 to 70 percent. The sedimentation profile of hnRNA labeled with (3H)uridine for 45 seconds after brief treatment (45, 90, or 180 seconds) with DRB showed a progressive decrease in the labeling of shorter hnRNA molecules relative to longer molecules. Prior exposure of the cells to actinomycin D, an inhibitor of RNA chain elongation, did not alter the sedimentation profile of hnRNA. These results suggest that DRB preferentially inhibits the initiation of hnRNA chains so that after exposure to DRB for a brief period the longer nascent chains still remain to be finished and thus incorporate a greater share of the pulse label. By progressively increasing the time of exposure to DRB, and measuring the rate of increase in the average size of the labeled, nascent RNA, it was estimated that the chains were growing at rates between 50 and 100 nucleotides per second.     

Lectin-like inhibition of immune complex receptor-mediated stimulation of neutrophils. Effects on cytosolic calcium release and superoxide production

We have tested the role of lectin-like interactions, with particular emphasis on CR3, in insoluble immune complex (IC)-mediated activation of human polymorphonuclear leukocytes (PMN). The ability of IC to trigger intracellular Ca2+ release and O2- production by normal PMN, saccharide-treated cells, and CR3-deficient PMN (leukocyte adhesion deficiency, LAD, patients) were tested. When indo-1-labeled normal PMN were stimulated with IC in Ca(2+)-free buffer, intracellular Ca2+ rose from approximately 100 nM to approximately 230 nM. However, when LAD PMN were tested, a small rise in intracellular Ca2+ was observed. Because previous studies have shown that certain saccharides inhibit CR3-Fc gamma RIII co-capping, we tested a panel of saccharides to determine their ability to influence IC-mediated intracellular Ca2+ release. When normal PMN were exposed to 0.15 M N-acetyl-D-glucosamine (NADG), D-mannose, or alpha-methyl-mannoside, the Ca2+ response to IC was significantly reduced. However, addition of 0.15 M glucose, raffinose, sucrose, galactose, fructose, or sorbitol did not significantly affect the Ca2+ response, suggesting that the response was specific for certain saccharides. To test the physiologic consequences of these Ca2+ signals, we have examined the ability of saccharides to influence O2- production by normal PMN and the ability of LAD PMN to produce O2- upon triggering by IC. Normal PMN stimulated with IC generated 4.3 +/- 1.7 nmol/10(6) cells/30 min of O2-. In contrast, O2- production was inhibited by 0 to 20% by glucose, galactose, sucrose, sorbitol, fructose, and raffinose and > or = 50% by NADG and mannose. LAD PMN, which display diminished Ca2+ signals, were found to produce O2- at 47 +/- 6% of control levels. NADG and mannose dose-response studies indicated that they cooperatively block O2-.     

Least-cost design of singly and doubly reinforced concrete beam using genetic algorithm optimized artificial neural network based on Levenberg–Marquardt and quasi-Newton backpropagation learning techniques

In this work, least-cost design of singly and doubly reinforced beams with uniformly distributed and concentrated load was done by incorporating actual self-weight of beam, parabolic stress block, moment–equilibrium and serviceability constraint besides other constraints. Also, this design expertise was incorporated into a genetically optimized artificial neural network based on steepest descent, Levenberg–Marquardt, and quasi-Newton backpropagation learning techniques. The initial solution for the optimization procedure was obtained using limit state design as per IS: 456-2000.     

High-level asymptotic variation of transistor base resistance and current gain

Using a finite-section model for bipolar transistors with current-dependent base resistance and gain, high-current asymptotic variations of base resistance, gain and effective emitter width are studied, and useful analytic formulas are presented.