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151.
PCR-based procedures in detection and DNA-fingerprinting of Salmonella from samples of animal origin
A. del Cerro S. M. Soto E. Landeras M. A. Gonzlez-Hevia J. A. Guijarro M. C. Mendoza 《Food microbiology》2002,19(6):567
The usefulness of selected PCR-protocols for the detection of Salmonella in 117 samples of animal origin (17 raw minced meat, 27 raw chicken meat, 8 raw sausages, and 25 egg samples, as well as 18 poultry faecal, and caecal swabs samples) and DNA-fingerprinting typing is shown. To establish an accurate PCR-procedure for Salmonella detection the following parameters were evaluated: two pre-PCR concentration procedures, centrifugation and immunomagnetic separation (IMS) using Dynabeads anti- Salmonella; the specificity and sensitivity of 10 sets of primers; and different conditions of the amplification reaction. In light of the results obtained from the use of PCR-based procedures alone or in combination with conventional methods, the following findings can be underlined: First, IMS is more efficient than centrifugation in the recovery of Salmonella. Second, the selected IMS/PCR-detection protocol is less time-consuming (45 h) than the IMS/culture procedure (90 h), and a good concordance between them was found when the Kappa coefficient was calculated (0·87). Third, PCR-ribotyping technique showed a very low discrimination power, being able to differentiate only three profiles. Fourth, RAPD technique using specific primers supports previous works in which it was proposed as a simple and useful tool for discriminating isolates between and within serotypes. Fifth, The efficiency, rapidity, and flexibility of the PCR-protocols applied were high, and they can be performed using two PCR-programs and the same basic equipment. 相似文献
152.
153.
The failure of the conductor of the 460 kV overhead transmission line located along the crossing of the Paraná River was investigated. As a result of this failure, a blackout took place in the southern states of Brazil in January of 2002, reaching approximately 67 million inhabitants. Investigation of the external aluminium layer of the ACSR conductor near the fracture showed typical static deformation marks and dynamic fretting wear tangential marks, both associated with the presence of Al2O3 debris. Additionally, encrusted silicon particles were observed on the external surface of the strands. The internal Al layer showed elliptical deformation marks also associated with Al2O3 debris. Both Al2O3 and Si particles are efficient abrasive material, which associated with slight relative motion of metallic surfaces (clamp/strand and strand/strand) can promote fretting wear. Two types of fracture surface of the Al strands were identified: 45° and quasi-normal surfaces, the former being the predominant type. Delamination and particle detachment were the mechanisms of superficial degradation observed on the Al strands, indicating that the rupture of the strands occurred under a gross slip fretting regime induced by sub-conductor oscillation. Inspection of the internal surface of as-cast Al–10%Si spacer clamps revealed different stages of intense circumferential wear caused by the preferential cracking and particle detachment of the eutectic constituent of the as-cast microstructure. This intense wear reduces the clamping contact pressure, which allows higher displacement amplitude and leads to the critical fretting regime. Finally, a few suggestions are discussed to minimise the occurrence of future failures. 相似文献
154.
The initial steps of the development of prestressed concrete containment (PCC) for nuclear power plant (NPP) with pressurized water reactors (PWR) in the former USSR are analyzed. The constructive and technological decisions, accepted for primary PCC of Novovoronez NPP, such as the positioning of reinforcement elements and seaths in cylindrical wall and dome of the containment, the anchorage of reinforcement element ends, the technological aspects of concrete works, system and technology of a high level of biaxial pressing on a thin-wall structure at large wrapping angles of power reinforcing strands and etc. are observed. Experience won through the construction and operation of the primary PCC served as a basis for development of a new generation of improved unified PCC (IUPCC) for serial NPP, equipped with PWR of capacity of 1000 MW. The IUPCC is actually a cylinder 45 m in diameter and 54-m high covered with a gently sloping spherical dome. Thickness of cylinder wall is 1200 mm and that of dome wall is 1100 mm. The principle novelty of this PCC is the type and positioning of reinforcement strands. The paper describes strand arrangement and their anchorage in IUPCC. In the vertical part of PCC, strands are arranged on a helical-loop scheme and both strand ends are firmly anchored at the ring girder. Each strand is bended at the bottom of the containment. In the dome, strands are grouped on the orthogonal-loop scheme with the anchorage on one side and with bend of loop on the opposite side of the ring girder. To prevent the leakage of gases and to ensure tightness of the IUPCC an inner metal 8-mm liner with special anti-corrosion coating is designed. Monitoring and checking the stress and strain state of IUPCC is possible during the building, testing as well as operating periods. If any defects or decreased prestress of concrete are detected it is possible to tighten or replace the strands. It is noted that the more than 20 IUPCCs are in-service in Russia, Ukraine, and Bulgaria where NPP with PWR of capacity of 1000 MW were constructed. 相似文献
155.
156.
This work is aimed at developing a predictive capability for the quantitative assessment of crack growth under fatigue loadings. The crack growth rate relation, , may involve all three stress intensity factors k1-k3 such that the direction of crack growth may not be known in advance and must be predicted from a preassumed criterion. In principle, both the stress amplitude and the mean stress level should be included in the original expression for .The strain energy density factor range, ΔS, is found to be a convenient parameter for predicting fatigue crack growth and can be applied expediently to examine the combined influence of crack geometry, complex loadings and material properties. Assumed is the accumulation of energy, , stored in an element ahead of the crack which triggers subcritical crack growth upon reaching a number of loading cycle, say ΔN. The proposed relationship includes both the stress amplitude and mean stress effects. 相似文献
157.
158.
159.
R. Sankarasubramanian C. S. Jog T. A. Abinandanan 《Metallurgical and Materials Transactions A》2002,33(4):1083-1090
We examine the symmetry-breaking transitions in equilibrium shapes of coherent precipitates in two-dimensional (2-D) systems
under a plane-strain condition with the principal misfit strain components ε*
xx
and ε*
yy
. For systems with cubic elastic moduli, we first show all the shape transitions associated with different values of t=ε*
yy
/ε*
xx
. We also characterize each of these transitions, by studying its dependence on elastic anisotropy and inhomogeneity. For
systems with dilatational misfit (t=1) and those with pure shear misfit (t=−1), the transition is from an equiaxed shape to an elongated shape, resulting in a break in rotational symmetry. For systems
with nondilatational misfit (−1<t<1; t ≠ 0), the transition involves a break in mirror symmetries normal to the x- and y-axes. The transition is continuous in all cases, except when 0<t<1. For systems which allow an invariant line (−1≤t<0), the critical size increases with an increase in the particle stiffness. However, for systems which do not allow an invariant
line (0<t≤1), the critical size first decreases, reaches a minimum, and then starts increasing with increasing particle stiffness;
moreover, the transition is also forbidden when the particle stiffness is greater than a critical value. 相似文献
160.