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31.
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Jens Hauslage Medea Abbrecht Lars Hanke Ruth Hemmersbach Claudia Koch Wolfgang Hanke Florian P. M. Kohn 《Microgravity science and technology》2016,28(6):633-638
All life on earth has been established under conditions of stable gravity of 1g. Nevertheless, in numerous experiments the direct gravity dependence of biological processes has been shown on all levels of organization, from single molecules to humans. To study the effects especially of microgravity on biological systems, a variety of platforms are available, from drop towers to the ISS. Due to the costs of these platforms and their limited availability, as an alternative, numerous simulators have been developed for so called “simulated” microgravity. A classical systems is a clinostat, basically rotating a sample around one axis, and by integration of the gravity vector for 360° arguing that thus the effects of gravity are depleted. Indeed, a variety of studies has shown that taking out the direction of gravity from a biological system often results in consequences similar to the exposure of the system to real microgravity. Nevertheless, the opposite has been shown, too, and as a consequence the relevance of clinostats in microgravity research is still under discussion. To get some more insight into this problem we have constructed a small fluorescence clinostat and have studied the effects of clinorotation on the cytosolic calcium concentration of neuroglioma cells. The results have been compared to experiments with identical cells in real microgravity, utilizing parabolic flight missions. Our results show that in case of a cell suspension used in a small florescence clinostat within a tube diameter of 2mm, the effects of clinorotation are comparable to those under real microgravity, both showing a significant increase in intracellular calcium concentration. 相似文献
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Jens Laube Valentin Baric Samir Salameh Lutz Mädler Lucio Colombi Ciacchi 《Granular Matter》2018,20(2):28
We develop a novel coarse-grained contact model for Discrete Element Method simulations of \(\hbox {TiO}_2\) nanoparticle films subjected to mechanical stress. All model elements and parameters are derived in a self-consistent and physically sound way from all-atom Molecular Dynamics simulations of interacting particles and surfaces. In particular, the nature of atomic-scale friction and dissipation effects is taken into account by explicit modelling of the surface features and water adsorbate layers that strongly mediate the particle-particle interactions. The quantitative accuracy of the coarse-grained model is validated against all-atom simulations of \(\hbox {TiO}_2\) nanoparticle agglomerates under tensile stress. Moreover, its predictive power is demonstrated with calculations of force-displacement curves of entire nanoparticle films probed with force spectroscopy. The simulation results are compared with Atomic Force Microscopy and Transmission Electron Microscopy experiments. 相似文献
35.
Oxidative Stress Imaging: Visualizing Oxidative Cellular Stress Induced by Nanoparticles in the Subcytotoxic Range Using Fluorescence Lifetime Imaging (Small 23/2018) 下载免费PDF全文
36.
Raimund Kirner Jens Knoop Adrian Prantl Markus Schordan Albrecht Kadlec 《Software and Systems Modeling》2011,10(3):411-437
Worst-case execution time (WCET) analysis is concerned with computing a precise-as-possible bound for the maximum time the execution of a program can
take. This information is indispensable for developing safety-critical real-time systems, e. g., in the avionics and automotive
fields. Starting with the initial works of Chen, Mok, Puschner, Shaw, and others in the mid and late 1980s, WCET analysis
turned into a well-established and vibrant field of research and development in academia and industry. The increasing number
and diversity of hardware and software platforms and the ongoing rapid technological advancement became drivers for the development
of a wide array of distinct methods and tools for WCET analysis. The precision, generality, and efficiency of these methods
and tools depend much on the expressiveness and usability of the annotation languages that are used to describe feasible and infeasible program paths. In this article we survey the annotation languages which
we consider formative for the field. By investigating and comparing their individual strengths and limitations with respect
to a set of pivotal criteria, we provide a coherent overview of the state of the art. Identifying open issues, we encourage
further research. This way, our approach is orthogonal and complementary to a recent approach of Wilhelm et al. who provide
a thorough survey of WCET analysis methods and tools that have been developed and used in academia and industry. 相似文献
37.
A modified design approach for compact ultra‐wideband microstrip filters with cascaded/folded stepped‐impedance resonators is described. The key feature of the proposed method is to facilitate stronger coupling between stepped‐impedance resonators and, at the same time, eliminate the requirement of extremely small gaps in coupled‐line sections, as found in traditional designs. Simulations and measurements demonstrate that the filters designed with this technique exhibit good reflection, insertion‐loss, and group‐delay performance within the 3.1–10.6 GHz band. © 2009 Wiley Periodicals, Inc. Int J RF and Microwave CAE 2010. 相似文献
38.
Liquid recirculation in microfluidic channels by the interplay of capillary and centrifugal forces 总被引:1,自引:1,他引:0
Jose L. Garcia-Cordero Lourdes Basabe-Desmonts Jens Ducrée Antonio J. Ricco 《Microfluidics and nanofluidics》2010,9(4-5):695-703
We demonstrate a technique to recirculate liquids in a microfluidic channel by alternating predominance of centrifugal and capillary forces to rapidly bring the entire volume of a liquid sample to within one diffusion length, δ, of the surface, even for sample volumes hundreds of times the product of δ and the geometric device area. This is accomplished by repetitive, random sampling of an on-disc sample reservoir to form a thin fluid layer of thickness δ in a microchannel, maintaining contact for the diffusion time, then rapidly exchanging the fluid layer for a fresh aliquot by disc rotation and stoppage. With this technique, liquid volumes of microlitres to millilitres can be handled in many sizes of microfluidic channels, provided the channel wall with greatest surface area is hydrophilic. We present a theoretical model describing the balance of centrifugal and capillary forces in the device and validate the model experimentally. 相似文献
39.
The steady-state simplified P
N
approximation to the radiative transfer equation has been successfully applied to many problems involving radiation. Recently,
time-dependent simplified P
N
equations have been derived by an asymptotic analysis similar to the asymptotic derivation of the steady-state SP
N
equations (Frank et al. in J. Comput. Phys. 226:2289–2305, 2007). In this paper, we present computational results for the time-dependent SP
N
equations in two dimensions, obtained by using an adaptive finite element approach. Several numerical comparisons with other
existing models are shown. 相似文献
40.