Reducing Water Seepage with Anionic Polyacrylamide: Application Methods and Turbidity Effects

Researchers used column and flume experiments with uniform silica sand with and without suspended solids to examine the extent and longevity of hydraulic conductivity reduction (HCR) resulting from three anionic polyacrylamide (PAM) application methods, liquid injection, slurry surface, and granular surface application. Low turbidity (0.4 NTU) liquid injection column tests showed 20–65% HCR, likely caused by an extensional viscosity mechanism. HCR increased as turbidity increased, especially at low PAM concentrations. High turbidity (100 NTU) liquid injection column tests showed 66–77% HCR, likely through surficial PAM–clay aggregate filtration. Column and adsorption tests strongly suggest PAM–sand adsorption does not cause substantial HCR. In low turbidity flume tests, PAM slurry application to the sand surface produced up to 100% HCR, likely through a viscosity mechanism, and granular application to the media surface produced up to 100% HCR, likely through fixed surficial polymer gel formation. Hydraulic conductivity returned to control-adjusted pretreatment levels following PAM application in all tests except granular surface application at high mass loads or with high turbidity.     

Impacts of global environmental change on future health and health care in tropical countries

The most common strategy in the development of HIV-1 protease inhibitors has been the design of high affinity transition state analogs that effectively compete with natural substrates for the active site. A second approach has been the development of compounds that inactivate the protease by destabilizing its quaternary or tertiary structure. A successful optimization of these strategies requires an accurate knowledge of the energetics of structural stabilization and binding, and the identification of those regions in the protease molecule that are critical to stability and function. Here the energetics of stabilization of the HIV-1 protease has been measured for the first time by high sensitivity differential scanning calorimetry. These studies have permitted the evaluation of the different components of the Gibbs energy of stabilization (the enthalpy, entropy and heat capacity changes). The stability of the protease is pH-dependent and due to its dimeric nature is also concentration-dependent. At pH 3.4 the Gibbs energy of stabilization is close to 10 kcal/mol at 25 degreesC, consistent with a dissociation constant of 5x10(-8) M. The stability of the protease increases at higher pH values. At pH 5, the Gibbs energy of stabilization is 14.5 kcal/mol at 25 degreesC, consistent with a dissociation constant of 2.3x10(-11) M. The pH dependence of the Gibbs energy of stabilization indicates that between pH 3.4 and pH 5 an average of 3-4 ionizable groups per dimer become protonated upon unfolding. A structure-based thermodynamic analysis of the protease molecule indicates that most of the Gibbs energy of stabilization is provided by the dimerization interface and that the isolated subunits are intrinsically unstable. The Gibbs energy, however, is not uniformly distributed along the dimerization interface. The dimer interface is characterized by the presence of clusters of residues (hot spots) that contribute significantly and other regions that contribute very little to subunit association. At the dimerization interface, residues located at the carboxy and amino termini contribute close to 75% of the total Gibbs energy (Cys95, Thr96, Leu97, Asn98 and Phe99 and Pro1, Ile3, Leu5). Residues Thr26, Gly27 and Asp29 located at the base of the active site are also important, and to a lesser extent Gly49, Ile50, Gly51 located at the tip of the flap region. The structure-based thermodynamic analysis also predicts the existence of regions of the protease with only marginal stability and a high propensity to undergo independent local unfolding. In particular, the flap region occupies a very shallow energy minimum and its conformation can easily be affected by relatively small perturbations. This property of the protease can be related to the ability of some mutations to elicit resistance towards certain inhibitors.     

Security in the wild: user strategies for managing security as an everyday,practical problem

Ubiquitous and mobile technologies create new challenges for system security. Effective security solutions depend not only on the mathematical and technical properties of those solutions, but also on people’s ability to understand them and use them as part of their work. As a step towards solving this problem, we have been examining how people experience security as a facet of their daily life, and how they routinely answer the question, “is this system secure enough for what I want to do?” We present a number of findings concerning the scope of security, attitudes towards security, and the social and organizational contexts within which security concerns arise, and point towards emerging technical solutions.     

Semiautomated depositor for infrared microspectrometry

The measurement of minute samples is desirable in many areas of research and analysis, including biological, environmental, and forensic sciences. The use of manual solution direct deposition, combined with surface evaporation, is a very useful and convenient method for the transfer of many analytes to a spectroscopic sampling window. For Fourier transform infrared (FT-IR) spectrometric analysis, high quality attenuated total reflection (ATR) and transmission analyses are possible with suitable solution deposits. If the sample area is very small, placement of the analyte deposit on the active area of the sampling accessory must be done carefully. To achieve this, a novel direct deposition system has been developed. This system, a series of valves attached to a glass nebulizer, generates deposits that are reproducible, and placement of the deposits is precise. The valves allow the sample to be loaded, helium airflow to expel the sample, and the nebulizer to be cleaned after deposition. To help contain the sample to a small area once deposition has taken place, a vacuum line is attached to the nebulizer. This simple semiautomated deposition system allows for higher sensitivity and run-to-run reproducibility for minute sample analysis.     

Local anesthetic treatment significantly attenuates acute pain responding but does not prevent the neonatal injury-induced reduction in adult spinal behavioral plasticity.

Recent findings indicate that neonatal injury results in decreased spinal plasticity in adult subjects (E. E. Young, K. M. Baumbauer, A. E. Elliot, & R. L. Joynes, 2007). Previous research has shown that acute manipulations of pain processing (i.e., administration of formalin, carrageenan, capsaicin) result in a loss of spinal behavioral plasticity (A. R. Ferguson, E. D. Crown, & J. W. Grau, 2006). Moreover, neonatal injury results in a lasting reduction in adult spinally mediated plasticity resembling the deficit seen following acute manipulations in adults (E. E. Young et al., 2007). The present study was designed to determine whether the effects of neonatal injury could be prevented by lidocaine administration during the initial healing period. Subjects (injured or uninjured) received lidocaine or saline on 1 of 4 administration schedules (preinjury only, postinjury only, for 24 hr postsurgery, or for 72 hr postsurgery). Results demonstrated that lidocaine administration did not prevent the hypersensitivity and reduced spinal plasticity associated with neonatal injury. This suggests that (a) the mechanisms underlying neonatal injury are independent of peripheral input in the initial healing period and (b) lidocaine is ineffective at preventing long-term spinal plasticity changes following neonatal injury. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Fluoxetine, smoking, and history of major depression: A randomized controlled trial.

The study was a randomized placebo-controlled trial testing whether fluoxetine selectively enhances cessation for smokers with a history of depression. Euthymic smokers with (H+, n = 109) or without (H-, n = 138) a history of major depression received 60 mg fluoxetine or placebo plus group behavioral quit-smoking treatment for 12 weeks. Fluoxetine initially enhanced cessation for H+ smokers (p = .02) but subsequently impaired cessation regardless of depressive history. Six months after quit date, fluoxetine-treated participants were 3.3 times more likely to be smoking (p = .02). Further research is warranted to determine why high-dose fluoxetine produces continuing effects that oppose tobacco abstinence. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Treating another's actions as one's own: Children's memory of and learning from joint activity.

Children often overestimate their contribution to collaborative activities. Across 2 studies, the authors investigated whether this memory bias supports internalization of the actions of others in the context of joint exchanges. After taking turns with (high collaborative condition; Studies 1 and 2) or working independently of (low collaborative condition; Study 2) an adult experimenter to create a series of novel toys, children's agent memory and reconstruction ability were assessed. Children in the high collaborative condition but not the low collaborative condition systematically overclaimed the actions of their social partner, more frequently reporting having completed steps performed by the experimenter than vice versa. This "I did it" bias was related to learning performance: high collaborative children outperformed low collaborative children both during an immediate reconstruction task and 4 months later, and the strength of the bias predicted children's independent toy-building accuracy. It is argued that the "I did it" bias may emerge as part of a general process of learning from others and is supported by a common framework for representing self-actions and other actions. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Optical Frequency Metrology of an Iodine-Stabilized He-Ne Laser Using the Frequency Comb of a Quantum-Interference-Stabilized Mode-Locked Laser

We perform optical frequency metrology of an iodine-stabilized He-Ne laser using a mode-locked Ti:sapphire laser frequency comb that is stabilized using quantum interference of photocurrents in a semiconductor. Using this technique, we demonstrate carrier-envelope offset frequency fluctuations of less than 5 mHz using a 1 s gate time. With the resulting stable frequency comb, we measure the optical frequency of the iodine transition [¹²⁷I₂ R(127) 11-5 i component] to be 473 612 214 712.96 ± 0.66 kHz, well within the uncertainty of the CIPM recommended value. The stability of the quantum interference technique is high enough such that it does not limit the measurements.     

Perturbed quantization steganography

In this paper, we use the recently proposed wet paper codes and introduce a new approach to passive-warden steganography called perturbed quantization. In perturbed quantization, the sender hides data while processing the cover object with an information-reducing operation that involves quantization, such as lossy compression, downsampling, or A/D conversion. The unquantized values of the processed cover object are considered as side information to confine the embedding changes to those unquantized elements whose values are close to the middle of quantization intervals. This choice of the selection channel calls for wet paper codes as they enable communication with non-shared selection channel. Heuristic is presented that indicates that the proposed method provides better steganographic security than current JPEG steganographic methods. This claim is further supported by blind steganalysis of a specific case of perturbed quantization for recompressed JPEG images.     

The predictive utility of hypnotizability: The change in suggestibility produced by hypnosis.

Objective: The predictive utility of hypnotizability, conceptualized as the change in suggestibility produced by a hypnotic induction, was investigated in the suggested reduction of experimental pain. Method: One hundred and seventy-three participants were assessed for nonhypnotic imaginative suggestibility. Thereafter, participants experienced hypnotic and nonhypnotic imaginative analgesia suggestions, counterbalanced for order. Hypnotic suggestibility was then assessed. Results: Hypnotizability, operationalized as hypnotic suggestibility with imaginative suggestibility statistically controlled (Braffman & Kirsch, 1999), predicted intraindividual differences in responding to the hypnotic and imaginative analgesia suggestions. Higher hypnotizability was associated with relatively greater response to the hypnotic analgesia suggestion than to the imaginative analgesia suggestion. Conclusions: Operationalized in this way, hypnotizability may be a useful predictor of the effect of adding a hypnotic induction to a specific imaginative suggestion. (PsycINFO Database Record (c) 2010 APA, all rights reserved)