Screening of supports for the immobilization of pectinmethylesterase from acerola (Malpighia glabra L)

A partially purified extract of pectinmethylesterase (PME) from acerola fruit was immobilized on various supports: glass, celite, chrysotile, agarose, concanavalin A Sepharose 4B, egg shell, polyacrylamide and gelatin. In addition, reticulation with glutaraldehyde was assessed, as well as the use of gelatin in the presence of celite, glass and silica. The highest immobilization yields were obtained when the pectinmethylesterase was immobilized in concanavalin A Sepharose 4B (81.7%) and in gelatin‐water (78.0%). Copyright © 2004 Society of Chemical Industry     

Effects of temperature, ammonium and glucose concentrations on yeast growth in a model wine system

In enology, alcoholic fermentation is a complex process involving several mechanisms. Slow and incomplete alcoholic fermentation is a chronic problem for the wine industry and factors leading to sluggish and stuck fermentations have been extensively studied and reviewed. The most studied cause of sluggish and stuck fermentation is the nitrogen content limitation. Nevertheless, other factors, such as temperature of fermentation and sugar concentration can affect the growth of yeasts. In this study we modelled the yeast growth‐cycle in wine model system as a function of temperature, sugar and ammonium concentrations; the individual effects and the interaction of these factors were analysed by means of a quadratic response surface methodology. Cell concentrations and weight loss were monitored in the whole wine fermentation process. The results of central composite design show that lower is the availability of nitrogen, higher is the cell growth rate; moreover, initial nitrogen concentration also influences survival time of Saccharomyces cerevisiae.     

Surface charge accumulation and decay on a spacer stressed with DC voltages at atmospheric conditions

Contents Surface charge accumulation and its decay at a GIS spacer are studied at atmospheric conditions employing a two step-calibration method. The results show that the accumulation saturates within 30 minutes; the dominant mechanism of surface charge accumulation is the drift of charged particles via air, while that of the decay is surface conduction. The characteristics are similar at both polarities.

---

Sättigungskonzentration und Abklingverhalten der Oberflächenladung an einem mit Gleichspannung beanspruchten Abstandshalter unter atmosphärischen Bedingungen

Übersicht Die Konzentration und das Abklingen von Oberflächenladungen an einem GIS Abstandshalter wurden unter atmosphärischen Bedingungen mit Hilfe eines zweistufigen Kalibrierungsverfahrens untersucht. Die Ergebnisse zeigen eine Sättigung der Ladungskonzentration innerhalb von 30 Minuten. Als Hauptursache der Ladungskonzentration ist die Drift geladener Teilchen in Luft anzusehen, während das Abklingen auf die Oberflächenleitung zurückzuführen ist. Ähnliche Charakteristiken ergeben sich mit beiden Polaritäten.

     

Smart help for operator performance

Our emphasis in this paper has been on the knowledge-based and cognitive issues pertinent for improved interactions between workers and manufacturing computer systems. We believe that these techniques are as necessary and applicable to manufacturing as to any other vocation, especially as the difference in collar color of shop people becomes less noticeable in their increasing role as knowledge workers.     

A systematic transition to flexible manufacturing

The transition from basic automation to flexible manufacturing is an expensive and tedious procedure. It requires meticulous planning and almost clairvoyant forecasting to insure that the initial flexibility obtained is sufficient to allow growth and expansion in the future. This paper will suggest a systematic and methodological approach to achieve optimal flexibility and describe the present results of its application to an ongoing system transformation.

Conclusions based on existing levels of completion are presented along with identification of critical and non critical flexibility requirements. The considerations and steps taken are summarized in a procedural format which may then be applied to a wide variety of system transformations.     

Intelligent structuring and exploration of digital music collections

In this paper we present a general approach to the automatic content-based organization and visualization of large digital music collections. The general methodology consists in extracting musically and perceptually relevant patterns (‘features’) from the given audio recordings (e.g., mp3 files), using topology-preserving data projection methods to map the entire music collection onto two-dimensional visualization planes (possibly in a hierarchical fashion), and using a new display metaphor (the ‘Islands of Music’) to display the inherent structure of the music collection to the user. It is shown how arbitrary meta-data can be integrated into the visualization process, and how similarity according to different viewpoints can be defined and exploited. The basic methodology is briefly described, three prototype systems are presented, and a general discussion of the practical application possibilities of such technologies is offered.     

Improving user security behaviour

Many organisations suspect that their internal security threat is more pressing than their external security threat. The internal threat is predominantly the result of poor user security behaviour. Yet, despite that, security awareness programmes often seem more likely to put users to sleep than to improve their behaviour. This article discusses the influences that affect a user's security behaviour and outlines how a well structured approach focused on improving behaviour could be an excellent way to take security slack out of an organisation and to achieve a high return for a modest, low-risk investment.     

Wasserstofffreie amorphe Kohlenstoffschichten ‐ abgeschieden durch plasmaaktivierte Hochratebedampfung

Hydrogen free amorphous carbon coatings ‐ deposited with plasma‐activated high‐rate evaporation By using the technology of the plasmaactivated electron beam evaporation we deposited hydrogen free amorphous carbon coatings with deposition rates of up to 100 μm/h. The results of the mechanical and tribological investigations show the potential of these layers for the use in case of wear resistance. Hardnesses of up to 16GPa were proved. The actual state of the characterisation of the coatings is described and the outlook onto possibilities of further optimisation is given.