Interfacial hybridization kinetics of oligonucleotides immobilized onto fused silica surfaces

Fused silica optical fibers have been used in an intrinsic mode optical configuration as biosensors for fluorescence based detection of hybridization of nucleic acids. In this work, the kinetics of hybridization of single-stranded oligonucleotides that were covalently immobilized were studied. The probe DNA was dT₂₀, and the target was Fluorescein-labeled non-complementary (dT₂₀) or complementary (dA₂₀) oligonucleotide. Chronofluorimetric monitoring of the adsorption and hybridization processes was used to investigate oligonucleotide films of different density, in different salt concentrations, at temperatures of 25 and 40 °C, with the concentration of the target DNA being 0.005–0.1 μM. Mathematical models based on first- and second-order Langmuir adsorption have been examined to describe both the adsorption and the hybridization processes. Experimental data were processed using the models, and the hybridization kinetics were calculated. Hybridization kinetics on these optical fiber DNA sensors was found to be up to three orders faster than results presented for a number of other experiments using different immobilization chemistries.     

关于变压器二次额定电压的计算

推导了变压器二次负载电压的计算公式,建议由此公式代替以往原理书中惯用的近似公式。利用此公式绘制和分析了不同功率因数额定负载时的相量轨迹图。     

一种高精度的CMOS带隙基准电压源

设计了一种采用0.25 μm CMOS工艺的高精度带隙基准电压源.该电路结构新颖,性能优异,其温度系数可达3×10-6/℃,电源抑制比可达75 dB.还增加了提高电源抑制比电路、启动电路和省功耗电路,以保证电路工作点正常、性能优良,并使电路的静态功耗较小.     

基于USB接口的射频IC卡系统

介绍了一种带有USB接口的射频IC卡系统,阐述了该系统的工作原理.经过方案对比,选用USBN9603作为USB接口控制芯片,构成USB接口电路,可以使设计简单,尤其适合于产品的改型设计.给出了USB设备的软件设计方法,并给出了单片机与上位管理机通信的程序流程.由于采用USB接口,可以克服以往IC卡系统不支持热插拔和不能灵活与外设连接的缺点.     

中兴宽带线路测试技术及功能介绍

宽带线路测试是中兴通讯ZXDSL综合宽带运维系统的核心功能，在DSL业务提供过程中主要应用在开通前的预测试、线路测试、开通后的例测试以及故障时的在线测试。对其测试技术和功能作了介绍。     

Optimization of support positions to maximize the fundamental frequency of structures

In this paper, the position optimization of simple supports is implemented to maximize the fundamental frequency of a beam or plate structure. Both elastic and rigid supports are taken into account. First, the frequency sensitivity with respect to the movement of a simple support is derived using the discrete method. By means of the shape functions of the finite element method, closed‐form sensitivity formulations are developed straightforwardly. Then, a heuristic approach, called evolutionary shift method, is presented for optimizing support positions with a fixed grid mesh scheme. Based on the design sensitivity analysis, the support with the highest efficiency is shifted in priority along the elementary edges with the interval (step) of the elementary size. To facilitate the convergence of the process, the interpolation technique is employed to evaluate the solution more accurately. Finally, three numerical examples are presented to demonstrate the validity of the sensitivity analysis and the effectiveness of the optimization method. Copyright © 2004 John Wiley & Sons, Ltd.     

济钢中厚板厂的技术改造与发展

结合济钢中厚板厂二期和三期工程、开发管线钢项目和大板坯连铸高效改造及炉外精炼改造，介绍了该厂的技术改造项目，对今后的发展进行了阐述．     

汽车代用燃料的应用和发展

评述了汽车代用燃料的现状和未来，压缩天然气和液化石油气将率先大量使用，其次是醇燃料，合成燃料和二甲醚将主要用于某些混合动力车，燃料电池因为能源效率高是最有吸引力的车用能源，将于2010-2015年开始在汽车上实际使用，我国石油工业在提升汽油和柴油质量的同时，应与汽车工业联手开发代用燃料，特别要注意发展燃料电池技术。