Application potential of bone marrow mesenchymal stem cell (BMSCs) based tissue-engineering for spinal cord defect repair in rat fetuses with spina bifida aperta

Spina bifida aperta are complex congenital malformations resulting from failure of fusion in the spinal neural tube during embryogenesis. Despite surgical repair of the defect, most patients who survive with spina bifida aperta have a multiple system handicap due to neuron deficiency of the defective spinal cord. Tissue engineering has emerged as a novel treatment for replacement of lost tissue. This study evaluated the prenatal surgical approach of transplanting a chitosan–gelatin scaffold seeded with bone marrow mesenchymal stem cells (BMSCs) in the healing the defective spinal cord of rat fetuses with retinoic acid induced spina bifida aperta. Scaffold characterisation revealed the porous structure, organic and amorphous content. This biomaterial promoted the adhesion, spreading and in vitro viability of the BMSCs. After transplantation of the scaffold combined with BMSCs, the defective region of spinal cord in rat fetuses with spina bifida aperta at E20 decreased obviously under stereomicroscopy, and the skin defect almost closed in many fetuses. The transplanted BMSCs in chitosan–gelatin scaffold survived, grew and expressed markers of neural stem cells and neurons in the defective spinal cord. In addition, the biomaterial presented high biocompatibility and slow biodegradation in vivo. In conclusion, prenatal transplantation of the scaffold combined with BMSCs could treat spinal cord defect in fetuses with spina bifida aperta by the regeneration of neurons and repairmen of defective region.     

Methylene blue photocatalytic degradation under visible irradiation of Al doped ZnO powders by hydrothermal synthesis sensitized with octa-iso-pentyloxyphthalocyanine lead

AZO powders were sensitized through chemisorption method by octa-iso-pentyloxy phthalocyanine lead and characterized by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. The results showed that after sensitization process AZO photo physical properties improved greatly in visible regions. Photocatalytic degradation of methylene blue was studied under visible irradiation in aqueous solution and the pseudo first order model was used to obtain kinetic information of the photocatalytic degradation. The results indicated that photocatalytic activities of PbPc(iso-PeO)₈-AZO were better than of AZO powders.     

基于Fluent的圆柱齿轮流量计的密封性仿真分析

通过对圆柱齿轮流量计进行二维简化建模,用Fluent仿真软件的ICEM CFD模块进行网格划分,利用Fluent仿真软件对圆柱齿轮流量计进行了计算和密封性的仿真分析,得出了其速度矢量分布图,并且给出了各泄漏量的计算公式和每种泄漏所占泄漏总量的百分比,即为以后的整个流量计的设计提供理论依据。,提高流量计的寿命具有重要意义。同时还省去了大量的人工计算过程,极大地加快了计算速度。     

Detwinning through migration of twin boundaries in nanotwinned Cu films under in situ ion irradiation

The mechanism of radiation-induced detwinning is different from that of deformation detwinning as the former is dominated by supersaturated radiation-induced defects while the latter is usually triggered by global stress. In situ Kr ion irradiation was performed to study the detwinning mechanism of nanotwinned Cu films with various twin thicknesses. Two types of incoherent twin boundaries (ITBs), so-called fixed ITBs and free ITBs, are characterized based on their structural features, and the difference in their migration behavior is investigated. It is observed that detwinning during radiation is attributed to the frequent migration of free ITBs, while the migration of fixed ITBs is absent. Statistics shows that the migration distance of free ITBs is thickness and dose dependent. Potential migration mechanisms are discussed.     

A Two‐Stage Annealing Strategy for Crystallization Control of CH3NH3PbI3 Films toward Highly Reproducible Perovskite Solar Cells

The solvent‐engineering method is widely used to fabricate top‐performing perovskite solar cells, which, however, usually exhibit inferior reproducibility. Herein, a two‐stage annealing (TSA) strategy is demonstrated for processing of perovskite films, namely, annealing the intermediate phase at 60 °C for the first stage then at 100 °C for the second stage. Compared to conventional direct annealing temperature (DHA) at 100 °C, using this strategy, MAPbI₃ films become more controllable, leading to superior film uniformity and device reproducibility with the champion device efficiency reaching 19.8%. More specifically, the coefficient of variation of efficiency for 49 cells is reduced to 5.9%, compared to 9.8% for that using DHA. The TSA process is carefully studied using Fourier transform infrared spectroscopy, X‐ray diffraction, and UV–vis absorption spectroscopy. It is found that in comparison with DHA the formation of hydrogen bonding and crystallization of perovskite are much slower and can be better controlled when using TSA. The improvements in film uniformity and device reproducibility are attributed to: 1) controllable MAPbI₃ crystal growth stemming from the progressive formation of hydrogen bonding between methylammonium and halide; 2) suppression of intermediate phase film dewetting, which is believed to be due to its decreased mobility at the initial low‐temperature annealing stage.