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71.
72.
Yu. G. Teterev 《Atomic Energy》1989,67(4):760-763
Translated from Atomnaya Énergiya, Vol. 67, No. 4, pp. 271–274, October, 1989. 相似文献
73.
A. N. Gudkov V. M. Zhivun A. V. Zvonarev V. V. Kovalenko A. B. Koldobskii Yu. F. Koleganov S. V. Krivasheev V. B. Pavlovich N. S. Piven' E. V. Semenova 《Atomic Energy》1989,66(2):115-118
Translated from Atomnaya Énergiya, Vol. 66, No. 2, pp. 100–103, February, 1989. 相似文献
74.
Translated from Atomnaya Énergiya, Vol. 67, No. 2, pp. 111–113, August, 1989. 相似文献
75.
PCR-based procedures in detection and DNA-fingerprinting of Salmonella from samples of animal origin
A. del Cerro S. M. Soto E. Landeras M. A. Gonzlez-Hevia J. A. Guijarro M. C. Mendoza 《Food microbiology》2002,19(6):567
The usefulness of selected PCR-protocols for the detection of Salmonella in 117 samples of animal origin (17 raw minced meat, 27 raw chicken meat, 8 raw sausages, and 25 egg samples, as well as 18 poultry faecal, and caecal swabs samples) and DNA-fingerprinting typing is shown. To establish an accurate PCR-procedure for Salmonella detection the following parameters were evaluated: two pre-PCR concentration procedures, centrifugation and immunomagnetic separation (IMS) using Dynabeads anti- Salmonella; the specificity and sensitivity of 10 sets of primers; and different conditions of the amplification reaction. In light of the results obtained from the use of PCR-based procedures alone or in combination with conventional methods, the following findings can be underlined: First, IMS is more efficient than centrifugation in the recovery of Salmonella. Second, the selected IMS/PCR-detection protocol is less time-consuming (45 h) than the IMS/culture procedure (90 h), and a good concordance between them was found when the Kappa coefficient was calculated (0·87). Third, PCR-ribotyping technique showed a very low discrimination power, being able to differentiate only three profiles. Fourth, RAPD technique using specific primers supports previous works in which it was proposed as a simple and useful tool for discriminating isolates between and within serotypes. Fifth, The efficiency, rapidity, and flexibility of the PCR-protocols applied were high, and they can be performed using two PCR-programs and the same basic equipment. 相似文献
76.
V. Yu. Tertychnyi-Dauri 《Automation and Remote Control》2002,63(1):76-89
The properties of the solutions of optimal parametric filtration equations and an adaptive variant of the problem with regard for time-drift of the unknown parameters of the system are studied. Along with the Kalman interpretation of the optimal adaptive nonlinear filter, the Bayes approach to applying the respective a posteriori densities is also studied. 相似文献
77.
78.
79.
80.
R. Sankarasubramanian C. S. Jog T. A. Abinandanan 《Metallurgical and Materials Transactions A》2002,33(4):1083-1090
We examine the symmetry-breaking transitions in equilibrium shapes of coherent precipitates in two-dimensional (2-D) systems
under a plane-strain condition with the principal misfit strain components ε*
xx
and ε*
yy
. For systems with cubic elastic moduli, we first show all the shape transitions associated with different values of t=ε*
yy
/ε*
xx
. We also characterize each of these transitions, by studying its dependence on elastic anisotropy and inhomogeneity. For
systems with dilatational misfit (t=1) and those with pure shear misfit (t=−1), the transition is from an equiaxed shape to an elongated shape, resulting in a break in rotational symmetry. For systems
with nondilatational misfit (−1<t<1; t ≠ 0), the transition involves a break in mirror symmetries normal to the x- and y-axes. The transition is continuous in all cases, except when 0<t<1. For systems which allow an invariant line (−1≤t<0), the critical size increases with an increase in the particle stiffness. However, for systems which do not allow an invariant
line (0<t≤1), the critical size first decreases, reaches a minimum, and then starts increasing with increasing particle stiffness;
moreover, the transition is also forbidden when the particle stiffness is greater than a critical value. 相似文献