Quantification of heart fatty acid binding protein as a biomarker for drug-induced cardiac and musculoskeletal necroses

Heart fatty acid binding protein (Fabp3) is a cytosolic protein expressed primarily in heart, and to a lesser extent in skeletal muscle, brain, and kidney. During myocardial injury, the Fabp3 level in serum is elevated rapidly, making it an ideal early marker for myocardial infarction. In this study, an MS‐based selected reaction monitoring method (LC‐SRM) was developed for quantifying Fabp3 in rat serum. Fabp3 was enriched first through an immobilized antibody, and the protein was digested on beads directly. A marker peptide of Fabp3 was quantified using LC‐SRM with a stable isotope‐labeled peptide standard. For six quality control samples with Fabp3 ranging from 0.256 to 25 ng, the average recovery following the procedure was about 73%, and the precision (%CV) between replicates was less than 7%. The Fabp3 concentrations in rat serum peaked 1 h after isoproterenol treatment, and returned to baseline levels 24 h after the dose. Elevated Fabp3 levels were also detected in rats administered with a PPAR α/δ agonist, which has shown to cause skeletal muscle necrosis. Fabp3 can be used as a biomarker for both cardiac and skeletal necroses. The cross‐validation of the LC‐SRM method with an existing ELISA method is described.     

Cholesterol is critical to the integrity of neuronal porosome/fusion pore

Secretion is one of the most fundamental cellular processes. Porosomes have been demonstrated as the universal secretory machinery in cells. Earlier studies determine the presence of a number of proteins in porosomes, among them the N- and P/Q-type calcium channels, actin, syntaxin-1, synaptotagmin-1, vimentin, the N-ethylmaleimide-sensitive factor (NSF), the chloride channel CLC-3, and the alpha subunit of the heterotrimeric GTP-binding protein G(o). Studies demonstrate that t-SNAREs localize at the base of porosomes, and directly interact with calcium channels. In the present study, we demonstrate that Syntaxin-1 co-localizes with cholesterol in solubilized synaptosomal membrane preparations. Depletion of cholesterol, results in the dissociation of both Syntaxin-1 and N-type calcium channel from neuronal porosomes. Thus, cholesterol participates as an integral component of the neuronal porosome complex, and is required for its stability.     

Beyond Knowledge Engineering

Knowledge engineering stems from E. A. Figenbaum＇s proposal in 1977, but it will enter a new decade with the new challenges. This paper first summarizes three knowledge engineering experiments we have undertaken to show possibility of separating knowledge development from intelligent software development. We call it the ICAX mode of intelligent application software generation. The key of this mode is to generate knowledge base, which is the source of intelligence of ICAX software, independently and parallel to intelligent software development. That gives birth to a new and more general concept ＂knowware＂. Knowware is a commercialized knowledge module with documentation and intellectual property, which is computer operable, but free of any built-in control mechanism, meeting some industrial standards and embeddable in software/hardware. The process of development, application and management of knowware is called knowware engineering. Two different knowware life cycle models are discussed： the furnace model and the crystallization model. Knowledge middleware is a class of software functioning in all aspects of knowware life cycle models. Finally, this paper also presents some examples of building knowware in the domain of information system engineering.     

（RbCl）108离子簇均相成核的分子动力学研究

分子动力学模拟测得熔融（RbCl)108离子簇在300K至600K温度时凝固的成核速率大于10^35m^-3s^-1,用均相成核的经典理论加以分析,估算得氯化铷离子簇的固液界面自由能σalT^1.4,用Grunasy提出的扩散界面理论加以分析,估算得扩展界面厚度约为0．195nm,σalT^0.97,2个理论在实验温度范围内没有明显判别,且均能较好地分子动力学模型的结果,但预测的较高温度下的成核速率有比较显著区别,因此进一步地研究将有助于鉴别它们。     

佩戴式虚拟现实技术

佩戴式虚拟现实系统可以让用户带着计算机进入真实世界 ,所以它对诸如医生、军人、消防队员等有着非常多的应用。本文介绍了佩戴式虚拟现实系统的概念、应用和产品 ,重点阐述了佩戴式虚拟现实系统的构成和关键技术     

虚拟企业支撑平台系统的设计

虚拟企业 (或曰动态企业联盟 )是目前公认的增强企业竞争力、对多变的全球市场进行快速反应的有效途径 ,受到普遍关注 .目前对虚拟企业技术的研究大多在模型以及支撑技术等方面 ,面向中小企业的虚拟企业构建支撑平台还没有得到应有的重视 ,然而这却是影响虚拟企业技术走向实用的关键 .本文在研究了基于 INTERNET/WEB虚拟企业模型基础上 ,深入研究了虚拟企业生命周期中各阶段的工作内容和特点 ,提出了基于 WEB的虚拟企业全生命周期支撑平台的系统概念和体系结构 ;同时详细介绍了我们开发的面向制造企业的虚拟企业生命周期的支撑平台系统 VESS.VESS将为我国制造企业快速构建自己的虚拟企业提供系统支撑 .     

基于图论的化学分子数据挖掘研究

近年来,人们提出了很多频繁图模式挖掘的算法。首先分析了贪婪搜索策略,然后对各种不同的图数据挖掘的方法进行比较。受购物篮分析的影响,基于ILP方法引起了人们的重视。如何修改各种不同的图数据挖掘方法以适用化学分子数据的挖掘是人们研究的热点问题。因为化学分子不仅是标准的图结构,而且它有典型的频繁环和链结构,还有一些频繁出现的代表原子类型的结点,所以在这个领域有一些特殊问题需要考虑。     

基于LIBPCAP的网络流量实时采集与信息萃取*

论述了网络流量实时采集与信息萃取的基本原理与实现方法。重点阐述在Linux环境下基于LIBPCAP包捕获机制,并在QT33平台上成功实现了基于GUI界面的网络流量实时采集与信息萃取及C++类的封装。     

On the convergence rate of ordinal comparisons of random variables

The asymptotic exponential convergence rate of ordinal comparisons follows from well-known results in large deviations theory, where the critical condition is the existence of a finite moment generating function. In this note, we show that this is both a necessary and sufficient condition, and also show how one can recover the exponential convergence rate in cases where the moment generating function is not finite. In particular, by working with appropriately truncated versions of the original random variables, the exponential convergence rate can be recovered     

Curie transition and piezoelectricity of the blends of a ferroelectric VDF/TrFE copolymer and PMMA

The Curie transition, even though the conformational change at the Curie transition primarily arises from intermolecular interaction, is highly dependent on the crystallization conditions. A slower cooling rate from the melt during paraelectric crystallization lowers T_c, increases the portion of F_β at the expense of F_α reduction, and produces a more unstable ferroelectric phase. T is rarely dependent upon the amount of PMMA, but T is increased with the PMMA content. PMMA has a favorable action in forming a more stable ferroelectric phase in the P(VDF/TrFE)/PMMA blend and elevating the Curie transition point because of the all-trans sequence conformation of PMMA and a specific intermolecular interaction with P(VDF/TrFE) in the melt state. However, PMMA reduces the total amount of the crystalline phase, the electric response, and the piezoelectricity. © 1993 John Wiley & Sons, Inc.